CELL INCORPORATION STUDY OF [99mTc]Tc-NAB-PACLITAXEL ON HUMAN COLORECTAL ADENOCARCINOMA CELL LINE

Meliha Ekinci; Derya İlem-özdemir

Makale detayları

CELL INCORPORATION STUDY OF [99mTc]Tc-NAB-PACLITAXEL ON HUMAN COLORECTAL ADENOCARCINOMA CELL LINE

Yazarlar:

Meliha Ekinci | Derya İlem-özdemir

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 1, pp.133-140

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001281535

pISSN:

1015-3918

Anahtar kelimeler:

Özet:

 Objective: The development of new radiopharmaceuticals capable of specifically imaging cancer is an important research area. For this reason, the aim of this study is to investigate cell binding of novel developed nanoradiopharmaceutical 99mTcTc-nab-PTX on HT-29 cell line human colorectal adenocarcinoma cells. Material and Method: In this study, nab-PTX was labeled with 99mTcTc, and labeling efficiency and in vitro stability were investigated by radioactive thin layer chromatography RTLC. Then, cell incorporation of 99mTcTc-nab-PTX was performed using HT-29 cell line. Result and Discussion: According to obtained results, nab-PTX was labeled with high radiochemical purity 99. The 99mTcTc-nab-PTX was found to be stable in saline for 6-h, and in cell medium up to 2-h. It was determined that 99mTcTc-nab-PTX had a greater cell binding activity on HT-29 cells than RH-99mTcNaTcO4 at 30 min. The results were found to be promising for future in vivo studies with 99mTcTc-nab-PTX.

 Objective: The development of new radiopharmaceuticals capable of specifically imaging cancer is an important research area. For this reason, the aim of this study is to investigate cell binding of novel developed nanoradiopharmaceutical 99mTcTc-nab-PTX on HT-29 cell line human colorectal adenocarcinoma cells. Material and Method: In this study, nab-PTX was labeled with 99mTcTc, and labeling efficiency and in vitro stability were investigated by radioactive thin layer chromatography RTLC. Then, cell incorporation of 99mTcTc-nab-PTX was performed using HT-29 cell line. Result and Discussion: According to obtained results, nab-PTX was labeled with high radiochemical purity 99. The 99mTcTc-nab-PTX was found to be stable in saline for 6-h, and in cell medium up to 2-h. It was determined that 99mTcTc-nab-PTX had a greater cell binding activity on HT-29 cells than RH-99mTcNaTcO4 at 30 min. The results were found to be promising for future in vivo studies with 99mTcTc-nab-PTX.

 Objective: The development of new radiopharmaceuticals capable of specifically imaging cancer is an important research area. For this reason, the aim of this study is to investigate cell binding of novel developed nanoradiopharmaceutical 99mTcTc-nab-PTX on HT-29 cell line human colorectal adenocarcinoma cells. Material and Method: In this study, nab-PTX was labeled with 99mTcTc, and labeling efficiency and in vitro stability were investigated by radioactive thin layer chromatography RTLC. Then, cell incorporation of 99mTcTc-nab-PTX was performed using HT-29 cell line. Result and Discussion: According to obtained results, nab-PTX was labeled with high radiochemical purity 99. The 99mTcTc-nab-PTX was found to be stable in saline for 6-h, and in cell medium up to 2-h. It was determined that 99mTcTc-nab-PTX had a greater cell binding activity on HT-29 cells than RH-99mTcNaTcO4 at 30 min. The results were found to be promising for future in vivo studies with 99mTcTc-nab-PTX.

 Amaç: Spesifik olarak kanseri görüntüleyebilen yeni radyofarmasötiklerin geliştirilmesi önemli bir araştırma alanıdır. Bu nedenle bu çalışmanın amacı, yeni geliştirilen nanoradyofarmasötiğin 99mTcTc-nab-PTX HT-29 hücre hattına insan kolorektal adenokarsinom hücreleri hücre bağlanmasını araştırmaktır. Materyal ve Metot: Bu çalışmada, nab-PTX 99mTcTc ile işaretlenmiş ve işaretleme etkinliği ve in vitro stabilitesi radyoaktif ince tabaka kromatografisi RTLC ile araştırılmıştır. Daha sonra, 99mTcTc-nab-PTXin hücre bağlanması, HT-29 hücre hattı kullanılarak gerçekleştirilmiştir. Sonuç ve Tartışma: Elde edilen sonuçlara göre, nab-PTX yüksek radyokimyasal saflıkla 99 radyoişaretlenmiştir. 99mTcTc-nab-PTXin oda sıcaklığında 6 saate kadar ve hücre ortamında 2 saate kadar stabil olduğu bulunmuştur. 99mTcTc-nab-PTXin HT-29 hücreleri üzerinde 30. dakikada RH-99mTcNaTcO4ten daha yüksek bir hücre bağlama aktivitesine sahip olduğu belirlenmiştir. Sonuçların 99mTcTc-nab-PTX ile gelecek in vivo çalışmalar için umut verici olduğu bulunmuştur.

Makale detayları

CELL INCORPORATION STUDY OF [99mTc]Tc-NAB-PACLITAXEL ON HUMAN COLORECTAL ADENOCARCINOMA CELL LINE

Yazarlar:

Meliha Ekinci | Derya İlem-özdemir

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 1, pp.133-140

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001281535

pISSN:

1015-3918

HT29 cell line

nab-paclitaxel

radiolabeling

technetium-99m

HT29 hücre hattı

nab-paklitaksel

radyoişaretleme

teknesyum-99m

Sayı

Makale detayları

Yazarlar: Meliha Ekinci | Derya İlem-özdemir

Yayıncı: Ankara University

Ana Başlıklar: Eczacılık ve İlaç Bilimleri

Dergi: Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 1, pp.133-140

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001281535

pISSN: 1015-3918

Sayı

Yazarlar:

Meliha Ekinci | Derya İlem-özdemir

Yayıncı:

Ankara University

Ana Başlıklar:

Eczacılık ve İlaç Bilimleri

Dergi:

Ankara Üniversitesi Eczacılık Fakültesi Dergisi Vol.47 Issue 1, pp.133-140

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001281535

pISSN:

1015-3918