Occurrence of enterocin genes, virulence factors, and antibiotic resistance in 3 bacteriocin-producer Enterococcus faecium strains isolated from Turkish tulum cheese

Banu Özden TUNCER; Zeliha AY; Yasin TUNCER

doi:https://doi.org/10.3906/biy-1209-26

Makale detayları

Occurrence of enterocin genes, virulence factors, and antibiotic resistance in 3 bacteriocin-producer Enterococcus faecium strains isolated from Turkish tulum cheese

Yazarlar:

Banu Özden TUNCER | Zeliha AY | Yasin TUNCER

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.443-449

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000812399

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1209-26

Anahtar kelimeler:

Özet:

 

In this study, the occurrence of enterocin genes, virulence factors, and antibiotic resistance in the bacteriocin-producer Enterococcus faecium EYT17, EYT31, and EYT39 strains was investigated. Polymerase chain reaction (PCR) studies showed that all of the E. faecium strains carried the enterocin A and B structural genes (entA and entB), while E. faecium EYT17 and EYT31 strains also carried the enterocin P structural gene (entP). None of the strains exhibited b-haemolysis or gelatinase activity. PCR analysis revealed that EYT17, EYT31, and EYT39 strains were clear of potential virulence determinants, except for ccf and efaAfm. The ccf gene was found in all of them. The efaAfm gene was only found in the EYT17 strain. All of the strains were found sensitive to ampicillin, chloramphenicol, gentamicin, norfloxacin, penicillin, streptomycin, tetracycline, sulphamethoxazole/trimethoprim, and vancomycin by disc diffusion method. E. faecium strains only exhibited intermediary resistance to erythromycin (15 µg). The results of this study showed that the multiple enterocin-producer E. faecium EYT17, EYT31, and EYT39 strains are safe and these strains may be used for food preservation.

 

In this study, the occurrence of enterocin genes, virulence factors, and antibiotic resistance in the bacteriocin-producer Enterococcus faecium EYT17, EYT31, and EYT39 strains was investigated. Polymerase chain reaction (PCR) studies showed that all of the E. faecium strains carried the enterocin A and B structural genes (entA and entB), while E. faecium EYT17 and EYT31 strains also carried the enterocin P structural gene (entP). None of the strains exhibited b-haemolysis or gelatinase activity. PCR analysis revealed that EYT17, EYT31, and EYT39 strains were clear of potential virulence determinants, except for ccf and efaAfm. The ccf gene was found in all of them. The efaAfm gene was only found in the EYT17 strain. All of the strains were found sensitive to ampicillin, chloramphenicol, gentamicin, norfloxacin, penicillin, streptomycin, tetracycline, sulphamethoxazole/trimethoprim, and vancomycin by disc diffusion method. E. faecium strains only exhibited intermediary resistance to erythromycin (15 µg). The results of this study showed that the multiple enterocin-producer E. faecium EYT17, EYT31, and EYT39 strains are safe and these strains may be used for food preservation.

Makale detayları

Occurrence of enterocin genes, virulence factors, and antibiotic resistance in 3 bacteriocin-producer Enterococcus faecium strains isolated from Turkish tulum cheese

Yazarlar:

Banu Özden TUNCER | Zeliha AY | Yasin TUNCER

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.443-449

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000812399

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1209-26

Key words: Enterococcus faecium

enterocin

virulence factors

antibiotic resistance

food safety

Sayı

Makale detayları

Occurrence of enterocin genes, virulence factors, and antibiotic resistance in 3 bacteriocin-producer Enterococcus faecium strains isolated from Turkish tulum cheese

Yazarlar: Banu Özden TUNCER | Zeliha AY | Yasin TUNCER

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.443-449

Belge Tipi: Makale

Yayın Tarihi: 3/2013

Belge Dili: İngilizce

Document ID (Org No.): 2000812399

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

DOI: https://doi.org/10.3906/biy-1209-26

Key words: Enterococcus faecium

enterocin

virulence factors

antibiotic resistance

food safety

Sayı

Yazarlar:

Banu Özden TUNCER | Zeliha AY | Yasin TUNCER

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2013 Vol.37 Issue 4, pp.443-449

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

İngilizce

Document ID (Org No.):

2000812399

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

DOI:

https://doi.org/10.3906/biy-1209-26