Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Bahar USLU; Burcu BİLTEKİN; Seçnur DENİR; Ayhan BİLİR; Suna  Özbaş TURAN; Jülide AKBUĞA; Serap ARBAK

Makale detayları

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Yazarlar:

Bahar USLU | Burcu BİLTEKİN | Seçnur DENİR | Ayhan BİLİR | Suna Özbaş TURAN | Jülide AKBUĞA | Serap ARBAK

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2015 Vol.32 Issue 1, pp.69-78

Belge Tipi:

Makale

Yayın Tarihi:

12/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000759938

Sıklık:

İki Aylık

pISSN:

2146-3123

Özet:

 

Background: Chitosan, a linear polysaccharide, has
 been recently used in biomedical applications. In vitro
 studies have demonstrated its effect on cellular growth
 and its stimulatory action on cellular layer formation.
 Aims: The present study aims to compare the proliferative
 effects of chitosan in two forms, membranous
 and solution forms, on Swiss 3T3 mouse embryonic
 fibroblasts.
 Study Design: In vitro study.
 Methods: Three experimental groups were formed:
 cells were cultured in a normal medium without
 chitosan (Control Group); cells were cultured either
 in a medium containing 2.0% chitosan in membranous
 form (Membrane Group) or chitosan solution
 at a concentration of 2.0% (Solution Group).
 Two different methods were used in the experiments:
 cells cultured on the medium containing
 chitosan in solution or membranous forms
 (method 1); and chitosan solution or membranous
 forms were added into the medium containing previously
 cultured cells (method 2).
 Results: Scanning electron microscopic investigations
 of the experimental groups revealed cells with welldefined
 cellular projections, intact cellular membranes
 and tight intercellular junctions. They were especially
 prominent in the membrane group of method 1 and in
 the membrane and solution groups of method 2. Mouse
 monoclonal anti-collagen 1 primary antibody was used 
 to indicate collagen synthesis. Prominent collagen synthesis
 was detected in the membrane groups on the 10th
 day of culture for both methods. Bromodeoxyuridine
 (BrdU) and MTT assays were performed in order to
 assess cellular proliferation and viability, respectively.
 BrdU labelling tests indicated a higher proliferation
 index in the membrane group of method 1 on the 5th
 and 10th days. For the second method, the membranous
 form on the 10th day and solution form on the 5th
 day were the most effective groups in terms of cellular
 proliferation. MTT results reflected a high cellular viability
 in method 1 on the 5th day of treatment with the
 membranous form, whereas cellular viability was highest
 in the solution form of method 2 on the 5th day.
 Conclusion: The membranous form of chitosan induced
 a significant proliferative effect and increased
 the ratio of cell-to-cell junctions of Swiss 3T3 mouse
 embryonic fibroblasts. Conveniently, the solution form
 also resulted in enhanced cell proliferation and viability
 compared to the control group. As the solution form
 is easy to prepare and apply to cells compared to the
 membrane form, the application of Chitosan directly to
 media appears to be a convenient alternative for tissue
 engineering approaches.

Makale detayları

Differences between Solution and Membrane Forms of Chitosan on the In Vitro Activity of Fibroblasts

Yazarlar: Bahar USLU | Burcu BİLTEKİN | Seçnur DENİR | Ayhan BİLİR | Suna Özbaş TURAN | Jülide AKBUĞA | Serap ARBAK

Yayıncı: Trakya University

Dergi: Balkan Medical Journal 2015 Vol.32 Issue 1, pp.69-78

Belge Tipi: Makale

Yayın Tarihi: 12/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000759938

Sıklık: İki Aylık

pISSN: 2146-3123

Sayı

Yazarlar:

Bahar USLU | Burcu BİLTEKİN | Seçnur DENİR | Ayhan BİLİR | Suna Özbaş TURAN | Jülide AKBUĞA | Serap ARBAK

Yayıncı:

Trakya University

Dergi:

Balkan Medical Journal 2015 Vol.32 Issue 1, pp.69-78

Belge Tipi:

Makale

Yayın Tarihi:

12/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000759938

Sıklık:

İki Aylık

pISSN:

2146-3123