Evaluation of GeneXpert vanA/vanB assay for the detection of vancomycin-resistant enterococci in patients newly admitted to intensive care units

Server YAĞCI; Çiğdem Ataman HATİPOĞLU; Şerife ALTUN; Cemal BULUT

Makale detayları

Evaluation of GeneXpert vanA/vanB assay for the detection of vancomycin-resistant enterococci in patients newly admitted to intensive care units

Yazarlar:

Server YAĞCI | Çiğdem Ataman HATİPOĞLU | Şerife ALTUN | Cemal BULUT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 6, pp.1008-1012

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753522

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

The automated Cepheid GeneXpert system provides rapid PCR results and can be used for the identification of VRE. We aimed to evaluate the use of the Cepheid Xpert vanA/vanB real-time PCR assay for the detection of VRE from rectal swabs of patients newly admitted to intensive care units in a hospital setting.  Materials and methods: Rectal swab samples of patients newly admitted to 6 intensive care units from March 2011 to February 2012 were obtained. The specimens were analyzed by the GeneXpert system. The results were reported for both vanA and vanB as negative or positive.   Results: Comparing the number of inpatients, most of the samples were delivered from the neurosurgery (48.3%), pediatrics (33.3%), and neonatology (20.6%) intensive care units. The positive samples according to GeneXpert vanA/vanB method were 33 (7.3%) among 454 rectal samples. Of these positive samples 22 (4.9%) were vanA, 10 (2.2%) were vanB, and 1 sample (0.2%) was vanA and vanB-harboring, by PCR.  Conclusion: As a rapid, easy to use, and labor-saving method GeneXpert vanA/vanB can detect VRE-positive patients, particularly in risk groups, as soon as they are admitted to hospital so that infection control policies can be applied immediately.

 

The automated Cepheid GeneXpert system provides rapid PCR results and can be used for the identification of VRE. We aimed to evaluate the use of the Cepheid Xpert vanA/vanB real-time PCR assay for the detection of VRE from rectal swabs of patients newly admitted to intensive care units in a hospital setting.  Materials and methods: Rectal swab samples of patients newly admitted to 6 intensive care units from March 2011 to February 2012 were obtained. The specimens were analyzed by the GeneXpert system. The results were reported for both vanA and vanB as negative or positive.   Results: Comparing the number of inpatients, most of the samples were delivered from the neurosurgery (48.3%), pediatrics (33.3%), and neonatology (20.6%) intensive care units. The positive samples according to GeneXpert vanA/vanB method were 33 (7.3%) among 454 rectal samples. Of these positive samples 22 (4.9%) were vanA, 10 (2.2%) were vanB, and 1 sample (0.2%) was vanA and vanB-harboring, by PCR.  Conclusion: As a rapid, easy to use, and labor-saving method GeneXpert vanA/vanB can detect VRE-positive patients, particularly in risk groups, as soon as they are admitted to hospital so that infection control policies can be applied immediately.

Makale detayları

Evaluation of GeneXpert vanA/vanB assay for the detection of vancomycin-resistant enterococci in patients newly admitted to intensive care units

Yazarlar:

Server YAĞCI | Çiğdem Ataman HATİPOĞLU | Şerife ALTUN | Cemal BULUT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 6, pp.1008-1012

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753522

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Vancomycin-resistant enterococci

GeneXpert vanA/vanB assay

intensive care unit

Sayı

Makale detayları

Evaluation of GeneXpert vanA/vanB assay for the detection of vancomycin-resistant enterococci in patients newly admitted to intensive care units

Yazarlar: Server YAĞCI | Çiğdem Ataman HATİPOĞLU | Şerife ALTUN | Cemal BULUT

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2013 Vol.43 Issue 6, pp.1008-1012

Belge Tipi: Makale

Yayın Tarihi: 5/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000753522

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Vancomycin-resistant enterococci

GeneXpert vanA/vanB assay

intensive care unit

Sayı

Yazarlar:

Server YAĞCI | Çiğdem Ataman HATİPOĞLU | Şerife ALTUN | Cemal BULUT

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2013 Vol.43 Issue 6, pp.1008-1012

Belge Tipi:

Makale

Yayın Tarihi:

5/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000753522

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144