Agrobacterium-mediated transformation of five inbred maize lines with the Brittle 2 gene

Thuy Ninh NGUYEN; Thi Luong TRAN; Duc Thanh NGUYEN

Makale detayları

Agrobacterium-mediated transformation of five inbred maize lines with the Brittle 2 gene

Yazarlar:

Thuy Ninh NGUYEN | Thi Luong TRAN | Duc Thanh NGUYEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 4, pp.755-761

Belge Tipi:

Makale

Yayın Tarihi:

7/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811605

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

The Brittle 2 (Bt2) gene encodes a small subunit of ADP-glucose pyrophosphorylase (AGPase) enzyme, one of the enzymes that play an important role in endosperm starch biosynthesis. We carried out a study to transfer the Bt2 gene into several inbred maize lines and regenerate transgenic maize plants possessing the transgene, which can be used to further study the role of the Bt2 gene in starch biosynthesis. Agrobacterium tumefaciens strains C58 and EHA105 harboring the transformation vector pCambia2300/Ubi-Bt2-Nos were used to transfer the Bt2 gene into five inbred maize lines, H14, H26, H240, H95, and N18. Transformed calli were selected on selection medium containing 100 mg/L of kanamycin. The stable integration of the Bt2 gene into transgenic plants was confirmed by Southern hybridizations. We recorded the different transformation frequencies in the experiments with A. Tumefaciens strains C58 and EHA105. Transgenic plants were regenerated from transformed calli of all five studied maize lines. Transformation frequencies varied from 1.09% to 2.33%, depending on maize genotypes. The results obtained show that our transformation system is effective for numerous maize genotypes. H95 and H240 are good lines for gene transfer in maize.

Makale detayları

Agrobacterium-mediated transformation of five inbred maize lines with the Brittle 2 gene

Yazarlar: Thuy Ninh NGUYEN | Thi Luong TRAN | Duc Thanh NGUYEN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2016 Vol.40 Issue 4, pp.755-761

Belge Tipi: Makale

Yayın Tarihi: 7/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000811605

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Thuy Ninh NGUYEN | Thi Luong TRAN | Duc Thanh NGUYEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 4, pp.755-761

Belge Tipi:

Makale

Yayın Tarihi:

7/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811605

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152