Investigation of the Effects of Rutin in Spraque Dawley Rats with Biochemical Parameters in Colistin-Induced Lung Injury

Özge Kandemir; Sefa Küçükler

Makale detayları

Investigation of the Effects of Rutin in Spraque Dawley Rats with Biochemical Parameters in Colistin-Induced Lung Injury

Yazarlar:

Özge Kandemir | Sefa Küçükler

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Metabolizması | Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.140-147

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328016

pISSN:

2149-6366

Anahtar kelimeler:

Özet:

 Colistin COL, frequently used for Gram-negative bacteria, may cause pulmonary toxicity in a dose-dependent manner. Flavanoid-type antioxidants have started to be used frequently against toxicity caused by different chemical agents. Rutin RUT is one of the flavanoid-type antioxidants. The present study aimed to investigate the effects of RUT in rats with COL-induced lung injury using biochemical parameters. In the experiment, 35 Spraqe Dawley rats were divided into five groups n7: Control, RUT, COL, COLRUT50, and COLRUT100. It was determined that COL increased lung tissue MDA values, decreased SOD, CAT, GPx activities, and GSH values, and triggered oxidative stress. COL administration increased NF-kB, TNF-α, IL-1β, MPO, and COX-2 levels, decreased mTOR levels, increased Beclin-1 levels and accelerated autophagy, increased Caspase-3 activity, and induced apoptosis. It was determined that RUT administration suppressed oxidative stress, inflammation, autophagy, and apoptosis by reversely regulating all these markers and reducing cell damage. The findings showed that the RUT application would be useful in COL-induced lung injury.

 Colistin COL, frequently used for Gram-negative bacteria, may cause pulmonary toxicity in a dose-dependent manner. Flavanoid-type antioxidants have started to be used frequently against toxicity caused by different chemical agents. Rutin RUT is one of the flavanoid-type antioxidants. The present study aimed to investigate the effects of RUT in rats with COL-induced lung injury using biochemical parameters. In the experiment, 35 Spraqe Dawley rats were divided into five groups n7: Control, RUT, COL, COLRUT50, and COLRUT100. It was determined that COL increased lung tissue MDA values, decreased SOD, CAT, GPx activities, and GSH values, and triggered oxidative stress. COL administration increased NF-kB, TNF-α, IL-1β, MPO, and COX-2 levels, decreased mTOR levels, increased Beclin-1 levels and accelerated autophagy, increased Caspase-3 activity, and induced apoptosis. It was determined that RUT administration suppressed oxidative stress, inflammation, autophagy, and apoptosis by reversely regulating all these markers and reducing cell damage. The findings showed that the RUT application would be useful in COL-induced lung injury.

 Colistin COL, frequently used for Gram-negative bacteria, may cause pulmonary toxicity in a dose-dependent manner. Flavanoid-type antioxidants have started to be used frequently against toxicity caused by different chemical agents. Rutin RUT is one of the flavanoid-type antioxidants. The present study aimed to investigate the effects of RUT in rats with COL-induced lung injury using biochemical parameters. In the experiment, 35 Spraqe Dawley rats were divided into five groups n7: Control, RUT, COL, COLRUT50, and COLRUT100. It was determined that COL increased lung tissue MDA values, decreased SOD, CAT, GPx activities, and GSH values, and triggered oxidative stress. COL administration increased NF-kB, TNF-α, IL-1β, MPO, and COX-2 levels, decreased mTOR levels, increased Beclin-1 levels and accelerated autophagy, increased Caspase-3 activity, and induced apoptosis. It was determined that RUT administration suppressed oxidative stress, inflammation, autophagy, and apoptosis by reversely regulating all these markers and reducing cell damage. The findings showed that the RUT application would be useful in COL-induced lung injury.

 Gram negatif bakteriler için sıklıkla kullanılan kolistin COL doza bağımlı olarak akciğer toksisitesinede neden olabilmektedir. Flavanoid türü antioksidanlar farklı kimyasal ajanların neden olduğu toksikasyonlara karşı günümüzde oldukça sık kullanılmaya başlamıştır. Rutin RUT flavanoid türü antioksidanlardan biridir. Sunulan çalışmada COL ile akciğer hasarı geliştirilen ratlarda RUTin etkilerinin biyokimyasal parametreler ile araştırılması amaçlanmıştır. Deneyde 35 adet Spraqe Dawley rat kontrol, RUT, COL, COLRUT 50 ve COLRUT100 olmak üzere 5 gruba n7 ayrıldı. COL in akciğer dokusu MDA değerlerini artırıp, SOD, CAT, GPx aktiviteleri ile GSH değerlerini azalttığı ve oksidatif stresi tetiklediği tespit edildi. COLuygulamasının, inflamasyon belirteçlerinden NF-kB, TNF-α, IL-1β, MPO ve COX-2 seviyelerini artırdığı, mTOR düzeylerinin azalıp ve Beclin-1 seviyelerini yükselterek otofajiyi hızlandırdığı, Caspase-3 aktivitesini artırarak apoptozisi indüklediği saptandı. RUT uygulamasının tüm bu belirteçleri tersine regüle ederek oksidatif stres, inflamasyon, otofaji ve apoptosizi baskıladığı ve hücredeki hasarı azalttığı belirlendi. Elde edilen bulgular COL kaynaklı akciğer hasarında RUT uygulamasının yararlı olacağını gösterdi.

Makale detayları

Investigation of the Effects of Rutin in Spraque Dawley Rats with Biochemical Parameters in Colistin-Induced Lung Injury

Yazarlar:

Özge Kandemir | Sefa Küçükler

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Metabolizması | Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.140-147

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328016

pISSN:

2149-6366

Lung injury

Colistin

Oxidative stress

RutinAkciğer hasarı

Kolistin

Oksidatif stres

Rutin

Sayı

Makale detayları

Yazarlar: Özge Kandemir | Sefa Küçükler

Yayıncı: Bingol University

Ana Başlıklar: Hücre Metabolizması | Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi: Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.140-147

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001328016

pISSN: 2149-6366

Sayı

Yazarlar:

Özge Kandemir | Sefa Küçükler

Yayıncı:

Bingol University

Ana Başlıklar:

Hücre Metabolizması | Biyokimya ve Hücre Biyolojisi (Diğer)

Dergi:

Türk Doğa ve Fen Dergisi Vol.13 Issue 3, pp.140-147

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001328016

pISSN:

2149-6366