Av ian  Salmonella enteritidis  serovar Enteritidis with  ClpP  deletion is attenuated and  highly immunogenic in chickens

Xuhua RAN; Zhiyuan YANG; Guangxi SHAO; Xiaobo WEN; Siguo LIU; Hongbo NI

Makale detayları

Av ian Salmonella enteritidis serovar Enteritidis with ClpP deletion is attenuated and highly immunogenic in chickens

Yazarlar:

Xuhua RAN | Zhiyuan YANG | Guangxi SHAO | Xiaobo WEN | Siguo LIU | Hongbo NI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.269-276

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000764579

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Özet:

 

Salmonella enterica subsp.
 enterica
 serovars are leading causative agents of gastroenteritis in humans and numerous animal 
species worldwide. The 
ClpP
 from 
Salmonella enterica
 is responsible for regulation under various stress conditions. We were interested in 
the effect of 
ClpP
 deletion mutation on attenuation of 
Salmonella enterica
 subsp.
 enterica
 serovar Enteritidis (SE) in chickens. Therefore, 
SM-6/Δ
ClpP
,  deletion  of  the  
ClpP
  gene  from  a  wild-type  SE-6  (SM-6)  strain,  was  generated  using  the  phage  &lamda;  Red  homologous  
recombination. Compared with SM-6, SM-6/&DeltaClpP
 represented a retarded growth rate and lower concentration in LB broth with and 
without  5%  NaCl  or  lower  pH  values,  indicating  that  it  is  sensitive  to  conventional  disinfectants.  SM-6/Δ
ClpP
  represented  reduced  
invasion into macrophages in vitro and an approximate 400-fold attenuation based on cell invasion assay and median lethal dose (LD
50
) 
in  specific  pathogen-free  (SPF)  chickens.  Furthermore,  SM-6/Δ
ClpP
  showed  reduced  colonization  in  the  liver  and  spleen  and  faster  
clearance compared with SM-6, indicating a short discharge. The survival rate of 7-day-old SPF chickens immunized with SM-6/Δ
ClpP 
against homologous challenge and antibody kinetics indicated that SM-6/ΔClpP
 provided efficient protection from lethal challenge and 
induced robust antibody immune responses, demonstrating the potential of live, attenuated SE
as a
vaccine candidate.

Makale detayları

Av ian Salmonella enteritidis serovar Enteritidis with ClpP deletion is attenuated and highly immunogenic in chickens

Yazarlar: Xuhua RAN | Zhiyuan YANG | Guangxi SHAO | Xiaobo WEN | Siguo LIU | Hongbo NI

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.269-276

Belge Tipi: Makale

Yayın Tarihi: 8/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000764579

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Sayı

Yazarlar:

Xuhua RAN | Zhiyuan YANG | Guangxi SHAO | Xiaobo WEN | Siguo LIU | Hongbo NI

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2018 Vol.42 Issue 4, pp.269-276

Belge Tipi:

Makale

Yayın Tarihi:

8/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000764579

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128