Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane  with the Assistance of polydopamine

Aykut Arif Topçu

Makale detayları

Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine

Yazarlar:

Aykut Arif Topçu

Yayıncı:

Hitit University

Dergi:

Hittite Journal of Science and Engineering Vol.10 Issue 1, pp.77-82

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287094

pISSN:

2148-4171

Anahtar kelimeler:

Özet:

 The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the polyhydroxyethyl methacrylate pHEMA cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy FTIR, scanning electron microscope SEM and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution. The maximum adsorption capacity Qmax of lysozyme was found as 108.9 mgg and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin BSA. Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.

 The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the polyhydroxyethyl methacrylate pHEMA cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy FTIR, scanning electron microscope SEM and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution. The maximum adsorption capacity Qmax of lysozyme was found as 108.9 mgg and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin BSA. Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.

 The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the polyhydroxyethyl methacrylate pHEMA cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy FTIR, scanning electron microscope SEM and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution. The maximum adsorption capacity Qmax of lysozyme was found as 108.9 mgg and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin BSA. Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.

 The surface imprinted technique was utilized in creating the synthetic lysozyme receptors on the polyhydroxyethyl methacrylate pHEMA cryogel membrane with the assistance of polydopamine under alkaline conditions. Following the design of the artificial receptors, Fourier-transform infrared spectroscopy FTIR, scanning electron microscope SEM and the swelling test were used for the characterization studies and the lysozyme adsorption capability of the adsorbent was evaluated in the aqueous solution. The maximum adsorption capacity Qmax of lysozyme was found as 108.9 mgg and the adsorption of the target protein on the adsorbent was monolayer and showed a good fit to the Langmuir isotherm model. The IF factor was 3.26 and the adsorbent was highly selective against creatinine and bovine serum albumin BSA. Furthermore, the adsorbent maintained its stability after 5 adsorption, desorption, and regeneration cycles.

Makale detayları

Lysozyme Imprinted poly(hydroxyethyl methacrylate) Cryogel Membrane with the Assistance of polydopamine

Yazarlar:

Aykut Arif Topçu

Yayıncı:

Hitit University

Dergi:

Hittite Journal of Science and Engineering Vol.10 Issue 1, pp.77-82

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287094

pISSN:

2148-4171

lysozyme

adsorption

molecular imprinting

p(HEMA)

dopamine

cryogel.

lysozyme

adsorption

molecular imprinting

p(HEMA)

dopamine

cryogel.

Sayı

Makale detayları

Yazarlar: Aykut Arif Topçu

Yayıncı: Hitit University

Dergi: Hittite Journal of Science and Engineering Vol.10 Issue 1, pp.77-82

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001287094

pISSN: 2148-4171

Sayı

Yazarlar:

Aykut Arif Topçu

Yayıncı:

Hitit University

Dergi:

Hittite Journal of Science and Engineering Vol.10 Issue 1, pp.77-82

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287094

pISSN:

2148-4171