Cytotoxic and antioxidant effects of paclitaxel and glutathione combination on breast cancer cell line

Gamze Sevri Ekren Aşıcı; İrem Bayar; Adem Yavaş; Ayşegül Bildik; Pınar Alkım Ulutaş

Makale detayları

Cytotoxic and antioxidant effects of paclitaxel and glutathione combination on breast cancer cell line

Yazarlar:

Gamze Sevri Ekren Aşıcı | İrem Bayar | Adem Yavaş | Ayşegül Bildik | Pınar Alkım Ulutaş

Yayıncı:

Adıyaman University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 3, pp.200-210

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334528

pISSN:

2458-9179

Anahtar kelimeler:

Özet:

 Aim: The aim of this study was to investigate the effects of glutathione GSH on chemotherapy-related toxicities in MCF-7 breast cancer cell line treated with paclitaxel PTX by cell viability and oxsidative stres parameters. Materials and Methods: Cells were treated with glutathione 2.5-20 mM and paclitaxel 0.001-100 µM for 24, 48 and 72 hours, after which cell viability was determined by WST-1 assay. IC50 values were calculated from the data obtained. Following combination analyses, the combination index was calculated and the levels of glutathione, total oxidant species TOS and total antioxidant species TAS were measured in cell lysates exposed to the indicated combinations for 72 hours. Results: In the study, it was determined that the cytotoxic effect of paclitaxel decreased as the amount of glutathion used in the combinations increased and glutathion concentrations above 10 mM showed antagonistic effect with paclitaxel. Conclusion: In patients with breast cancer, the administration of appropriate doses of glutathione in combination with chemotherapy may prove beneficial in reducing the adverse effects associated with oxidative stress.

 Aim: The aim of this study was to investigate the effects of glutathione GSH on chemotherapy-related toxicities in MCF-7 breast cancer cell line treated with paclitaxel PTX by cell viability and oxsidative stres parameters. Materials and Methods: Cells were treated with glutathione 2.5-20 mM and paclitaxel 0.001-100 µM for 24, 48 and 72 hours, after which cell viability was determined by WST-1 assay. IC50 values were calculated from the data obtained. Following combination analyses, the combination index was calculated and the levels of glutathione, total oxidant species TOS and total antioxidant species TAS were measured in cell lysates exposed to the indicated combinations for 72 hours. Results: In the study, it was determined that the cytotoxic effect of paclitaxel decreased as the amount of glutathion used in the combinations increased and glutathion concentrations above 10 mM showed antagonistic effect with paclitaxel. Conclusion: In patients with breast cancer, the administration of appropriate doses of glutathione in combination with chemotherapy may prove beneficial in reducing the adverse effects associated with oxidative stress.

 Aim: The aim of this study was to investigate the effects of glutathione GSH on chemotherapy-related toxicities in MCF-7 breast cancer cell line treated with paclitaxel PTX by cell viability and oxsidative stres parameters. Materials and Methods: Cells were treated with glutathione 2.5-20 mM and paclitaxel 0.001-100 µM for 24, 48 and 72 hours, after which cell viability was determined by WST-1 assay. IC50 values were calculated from the data obtained. Following combination analyses, the combination index was calculated and the levels of glutathione, total oxidant species TOS and total antioxidant species TAS were measured in cell lysates exposed to the indicated combinations for 72 hours. Results: In the study, it was determined that the cytotoxic effect of paclitaxel decreased as the amount of glutathion used in the combinations increased and glutathion concentrations above 10 mM showed antagonistic effect with paclitaxel. Conclusion: In patients with breast cancer, the administration of appropriate doses of glutathione in combination with chemotherapy may prove beneficial in reducing the adverse effects associated with oxidative stress.

 Amaç: Bu çalışmanın amacı Paklitaksel PTX ile tedavi edilen MCF-7 meme kanseri hücre hattında glutatyonun GSH kemoterapi ile ilişkili toksisiteler üzerindeki etkilerini hücre canlılığı ve oksidatif stres parametreleri ile araştırmaktır. Gereç ve Yöntem: Hücreler 24, 48 ve 72 saat boyunca glutatyon 2,5-20 mM ve paklitaksel 0,001-100 µM ile muamele edilmiş, ardından hücre canlılığı WST-1 testi ile belirlenmiştir. Elde edilen verilerden IC50 değerleri hesaplanmıştır. Kombinasyon analizlerinin ardından kombinasyon indeksi hesaplanmış ve 72 saat boyunca belirtilen kombinasyonlara maruz bırakılan hücre lizatlarında glutatyon, toplam oksidan türler TOS ve toplam antioksidan türler TAS seviyeleri ölçülmüştür. Bulgular: Çalışmada, kombinasyonlarda kullanılan glutatyon miktarı arttıkça paklitakselin sitotoksik etkisinin azaldığı ve 10 mM üzerindeki glutatyon konsantrasyonlarının paklitaksel ile antagonistik etki gösterdiği tespit edilmiştir. Sonuç: Meme kanseri hastalarında, kemoterapi ile birlikte uygun dozlarda glutatyon uygulanması, oksidatif stres ile ilişkili olumsuz etkileri azaltmada faydalı olabilir.

Makale detayları

Cytotoxic and antioxidant effects of paclitaxel and glutathione combination on breast cancer cell line

Yazarlar:

Gamze Sevri Ekren Aşıcı | İrem Bayar | Adem Yavaş | Ayşegül Bildik | Pınar Alkım Ulutaş

Yayıncı:

Adıyaman University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 3, pp.200-210

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334528

pISSN:

2458-9179

Antioxidant

Glutathione

Cancer

MCF-7

PaclitaxelAntioksidan

Glutatyon

Kanser

MCF-7

Paklitaksel

Sayı

Makale detayları

Yazarlar: Gamze Sevri Ekren Aşıcı | İrem Bayar | Adem Yavaş | Ayşegül Bildik | Pınar Alkım Ulutaş

Yayıncı: Adıyaman University

Ana Başlıklar: Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi: Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 3, pp.200-210

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001334528

pISSN: 2458-9179

Sayı

Yazarlar:

Gamze Sevri Ekren Aşıcı | İrem Bayar | Adem Yavaş | Ayşegül Bildik | Pınar Alkım Ulutaş

Yayıncı:

Adıyaman University

Ana Başlıklar:

Hücre Gelişimi | Proliferasyon ve Ölümü

Dergi:

Adıyaman Üniversitesi Sağlık Bilimleri Dergisi Vol.10 Issue 3, pp.200-210

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001334528

pISSN:

2458-9179