α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.

Sıla Sener; Şeyda Kanbolat; Nevin Ulaş Çolak; Merve Badem; Rezzan Aliyazıcıoğlu; Ufuk Özgen; Ali Kandemir

Makale detayları

α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.

Yazarlar:

Sıla Sener | Şeyda Kanbolat | Nevin Ulaş Çolak | Merve Badem | Rezzan Aliyazıcıoğlu | Ufuk Özgen | Ali Kandemir

Yayıncı:

Trakya University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.41-49

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287853

pISSN:

2528-9691

Anahtar kelimeler:

Özet:

 Obesity, defined as New World Syndrome, causes global health problems and big economic losses. Natural products have gained increasing importance because of their antiobesity potency. The genus Jurinea Cass. with approximately 200 described species worldwide has been traditionally used as a therapeutic agent for colic, fever, gout and rheumatism. The aim of this study was to analyze the volatile components, to determine phenolic compounds and to evaluate α-amylase, α-glucosidase, and lipase inhibitory activities of the endemic plant species Jurinea brevicaulis Boiss. The widely used solid-phase microextraction technique SPME was employed for Gas Chromatography-Mass Spectrometry GCMS analysis of the volatile components. Quantitative analysis of phenolic compounds was performed using Reversed-Phase High-Performance Liquid Chromatography RP-HPLC. A total of 19 volatile components were specified and o-cymene 10.60 , β-bisabolene 9.30 , and sesquicineole 57.5  for different terpenes were described as major components. According to the RP-HPLC analysis, sinapic acid, p-coumaric acid and quercetin were determined for the species. IC50 values of the species were determined as 36.59  2.37 μgmL and 42.56  2.83 μgmL for α-amylase and α-glucosidase assays, respectively. IC50 value was found as 50.31  3.75 μgmL with the lipase inhibition analysis. In conclusion, it has been determined that J. brevicaulis included diverse volatile components, three phenolic compounds with antiobesity effect potential, which highlights J. brevicaulis as the up-and-coming candidate of natural product source to be used against obesity.

 Obesity, defined as New World Syndrome, causes global health problems and big economic losses. Natural products have gained increasing importance because of their antiobesity potency. The genus Jurinea Cass. with approximately 200 described species worldwide has been traditionally used as a therapeutic agent for colic, fever, gout and rheumatism. The aim of this study was to analyze the volatile components, to determine phenolic compounds and to evaluate α-amylase, α-glucosidase, and lipase inhibitory activities of the endemic plant species Jurinea brevicaulis Boiss. The widely used solid-phase microextraction technique SPME was employed for Gas Chromatography-Mass Spectrometry GCMS analysis of the volatile components. Quantitative analysis of phenolic compounds was performed using Reversed-Phase High-Performance Liquid Chromatography RP-HPLC. A total of 19 volatile components were specified and o-cymene 10.60 , β-bisabolene 9.30 , and sesquicineole 57.5  for different terpenes were described as major components. According to the RP-HPLC analysis, sinapic acid, p-coumaric acid and quercetin were determined for the species. IC50 values of the species were determined as 36.59  2.37 μgmL and 42.56  2.83 μgmL for α-amylase and α-glucosidase assays, respectively. IC50 value was found as 50.31  3.75 μgmL with the lipase inhibition analysis. In conclusion, it has been determined that J. brevicaulis included diverse volatile components, three phenolic compounds with antiobesity effect potential, which highlights J. brevicaulis as the up-and-coming candidate of natural product source to be used against obesity.

 Obesity, defined as New World Syndrome, causes global health problems and big economic losses. Natural products have gained increasing importance because of their antiobesity potency. The genus Jurinea Cass. with approximately 200 described species worldwide has been traditionally used as a therapeutic agent for colic, fever, gout and rheumatism. The aim of this study was to analyze the volatile components, to determine phenolic compounds and to evaluate α-amylase, α-glucosidase, and lipase inhibitory activities of the endemic plant species Jurinea brevicaulis Boiss. The widely used solid-phase microextraction technique SPME was employed for Gas Chromatography-Mass Spectrometry GCMS analysis of the volatile components. Quantitative analysis of phenolic compounds was performed using Reversed-Phase High-Performance Liquid Chromatography RP-HPLC. A total of 19 volatile components were specified and o-cymene 10.60 , β-bisabolene 9.30 , and sesquicineole 57.5  for different terpenes were described as major components. According to the RP-HPLC analysis, sinapic acid, p-coumaric acid and quercetin were determined for the species. IC50 values of the species were determined as 36.59  2.37 μgmL and 42.56  2.83 μgmL for α-amylase and α-glucosidase assays, respectively. IC50 value was found as 50.31  3.75 μgmL with the lipase inhibition analysis. In conclusion, it has been determined that J. brevicaulis included diverse volatile components, three phenolic compounds with antiobesity effect potential, which highlights J. brevicaulis as the up-and-coming candidate of natural product source to be used against obesity.

 Yeni dünya sendromu olarak tanımlanan obezite, küresel sağlık sorunlarına ve büyük ekonomik kayıplara neden olmaktadır. Antiobezite potansiyelleri nedeniyle doğal ürünler, giderek artan bir önem kazanmaktadır. Jurinea Cass. cinsi dünya çapında yaklaşık 200 tür içermektedir ve geleneksel olarak kolik, ateş, gut ve romatizma için terapötik bir ajan olarak kullanılmaktadır. Bu araştırma ile Jurinea brevicaulis Boissin uçucu bileşenlerinin analiz edilmesi, fenolik bileşiklerinin belirlenmesi ve α-amilaz, α-glukozidaz ve lipaz inhibitör etkilerinin değerlendirilmesi amaçlanmıştır. Jurinae brevicaulisin uçucu bileşenlerinin Gaz Kromatografisi-Kütle Spektrometresi GCMS analizi için katı-faz mikro ekstraksiyon tekniği SPME kullanılmıştır. Jurinae brevicaulisin fenolik bileşiklerinin kantitatif analizi, Yüksek performanslı ters faz sıvı kromatografisi RP-HPLC kullanılarak yapılmıştır. Toplam 19 uçucu bileşen belirlenmiş ve farklı terpenler için o-simen 10.60, β-bisabolen 9,30 ve seskisinol 57,5 ana bileşenler olarak tanımlanmıştır. Tür için RP-HPLC analizine göre sinapik asit, p-kumarik asit ve kuersetin belirlenmiştir. Türün IC50 değerleri α-amilaz ve α-glukozidaz deneyleri için sırasıyla 36,59  2,37 μgmL ve 42,56  2,83 μgmL olarak bulunmuştur. Lipaz inhibisyon IC50 değeri 50,31  3,75 μgmL bulunmuştur. Sonuç olarak, J. brevicaulisin antiobezite etki potansiyeline sahip üç fenolik bileşik içerdiği, bunun da J. brevicaulisi obeziteye karşı kullanılacak doğal ürün kaynağı olarak gelecek vadeden bir aday olarak öne çıkardığı belirlenmiştir.

Makale detayları

α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.

Yazarlar:

Sıla Sener | Şeyda Kanbolat | Nevin Ulaş Çolak | Merve Badem | Rezzan Aliyazıcıoğlu | Ufuk Özgen | Ali Kandemir

Yayıncı:

Trakya University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.41-49

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287853

pISSN:

2528-9691

RP-HPLC

Lipase

Jurinea

Antiobesity

SPME

GC/MS

Sayı

Makale detayları

α-AMYLASE, α-GLUCOSIDASE AND LIPASE INHIBITORY PROPERTIES AND PHYTOCHEMICAL ANALYSIS OF ENDEMIC PLANT Jurinea brevicaulis Boiss.

Yazarlar: Sıla Sener | Şeyda Kanbolat | Nevin Ulaş Çolak | Merve Badem | Rezzan Aliyazıcıoğlu | Ufuk Özgen | Ali Kandemir

Yayıncı: Trakya University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences

Dergi: Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.41-49

Belge Tipi: Makale

Yayın Tarihi: 4/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001287853

pISSN: 2528-9691

RP-HPLC

Lipase

Jurinea

Antiobesity

SPME

GC/MS

Sayı

Yazarlar:

Sıla Sener | Şeyda Kanbolat | Nevin Ulaş Çolak | Merve Badem | Rezzan Aliyazıcıoğlu | Ufuk Özgen | Ali Kandemir

Yayıncı:

Trakya University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

Trakya University Journal of Natural Sciences Vol.24 Issue 1, pp.41-49

Belge Tipi:

Makale

Yayın Tarihi:

4/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287853

pISSN:

2528-9691