Crm1 knockdown by specific small interfering RNA reduces cell proliferation and induces apoptosis in head and neck cancer cell lines

Sibel ÖZDAŞ; Talih ÖZDAŞ

Makale detayları

Crm1 knockdown by specific small interfering RNA reduces cell proliferation and induces apoptosis in head and neck cancer cell lines

Yazarlar:

Sibel ÖZDAŞ | Talih ÖZDAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 2, pp.132-143

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811241

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

 
                         Head and neck squamous cell carcinoma (HNSCC) is the most common and most aggressive type of head and neck cancer. 
Current  approaches  for  the  treatment  of  HNSCC  are  not  sufficient  to  increase  the  patient  survival  or  to  reduce  the  high  recurrence  
rate.  Consequently,  there  is  a  need  to  explore  the  molecular  characteristics  of  this  cancer  in  order  to  discover  potential  therapeutic  
target molecules. The overexpression of chromosome region maintenance 1 (Crm1), responsible for the transport of different classes of 
macromolecules from the nuclear membrane to the cytoplasm, in various cancer cells has made it an attractive target molecule in cancer 
research. It has been reported that transcription factors, which are the target cargo proteins of Crm1, have critical roles in regulating 
intracellular  processes  via  their  expression  levels  and  functions,  which  in  turn  are  regulated  by  the  cell  cycle  and  signaling  proteins.  
Previous  findings  show  that  head  and  neck  cancer  cells  overexpress  Crm1  and  that  these  cells  become  highly  dependent  on  Crm1  
function.  The  results  of  this  study  show  that  after  decreasing  Crm1  expression  levels  in  HNSCC  cells  through  either  treatment  with  
specific Crm1 RNA interference (siRNA) or the selective Crm1 inhibitor leptomycin B (LMB), cell viability, proliferation, migration, 
and  wound-healing  abilities  decreased,  suppressing  tumorigenic  properties  through  the  induction  of  apoptosis.  Crm1  is  a  powerful  
diagnostic  biomarker  because  of  its  central  role  in  cancerogenesis,  and  it  has  a  high  potential  for  the  development  of  targeted  Crm1  
molecules or synthetic agents, such as LMB, as well as for the improvement of the clinical features in head and neck cancer.

Makale detayları

Crm1 knockdown by specific small interfering RNA reduces cell proliferation and induces apoptosis in head and neck cancer cell lines

Yazarlar: Sibel ÖZDAŞ | Talih ÖZDAŞ

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2018 Vol.42 Issue 2, pp.132-143

Belge Tipi: Makale

Yayın Tarihi: 4/2018

Belge Dili: İngilizce

Document ID (Org No.): 2000811241

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Sibel ÖZDAŞ | Talih ÖZDAŞ

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2018 Vol.42 Issue 2, pp.132-143

Belge Tipi:

Makale

Yayın Tarihi:

4/2018

Belge Dili:

İngilizce

Document ID (Org No.):

2000811241

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152