Determination of 8-OHdG and 4-HNE Expressions in Sheep with Hepatic Lipidosis by Immunohistochemical Method

Özhan Karataş; Gökhan Akçakavak; Murat Eren; Zeynep Çelik; Ayşenur Tural; Osman Dağar; Osman Doğan; Bahadır Kılınç; Mehmet Tuzcu

Makale detayları

Determination of 8-OHdG and 4-HNE Expressions in Sheep with Hepatic Lipidosis by Immunohistochemical Method

Yazarlar:

Özhan Karataş | Gökhan Akçakavak | Murat Eren | Zeynep Çelik | Ayşenur Tural | Osman Dağar | Osman Doğan | Bahadır Kılınç | Mehmet Tuzcu

Yayıncı:

Kyrgyz-Turkish Manas University

Ana Başlıklar:

Veteriner Patoloji

Dergi:

Manas Journal of Agriculture Veterinary and Life Sciences Vol.15 Issue 1, pp.9-15

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352125

pISSN:

1694-7932

Anahtar kelimeler:

Özet:

 Lipidosis is generally defined as the accumulation of triglycerides in limited droplets within the cytoplasm of parenchymal cells. Lipidosis occurs due to toxic, chemical, infectious, and metabolic causes. This study aimed to reveal the local 8-hydroxy-2-deoxyguanosine 8-OHdG and 4-hydroxynonenal 4-HNE expressions immunohistochemically according to the severity of the disease in sheep with hepatic lipidosis. The study material consisted of a total of 30 male sheep livers, including 6 healthy and 24 with hepatic lipidosis. After the liver samples were fixed in 10 formaldehyde solution, they underwent routine tissue processing to obtain paraffin blocks. Sections taken from the paraffin blocks were then subjected to Hematoxylin-Eosin H-E and immunohistochemical staining. Microscopically, control group liver samples showed normal histology. In livers with hepatic lipidosis, sharp-edged vacuoles of various sizes were detected in hepatocytes, and cell nuclei were pushed to the periphery. Additionally, focal hemorrhage and congestion, inflammatory cell infiltration in the portal area, bile duct proliferation, and connective tissue cells were observed. Microscopically, hepatic lipidosis cases were divided into two groups as moderate and severe based on the distribution of vacuoles in the section. In the immunohistochemical examination, 8-OHdG and 4-HNE expressions significantly increased in hepatic lipidosis cases compared to the control group p0.001. More intense immunoreactivity was detected especially in cases where disease severity increased p0.001. These results indicate that 8-OHdG and 4-HNE proteins play an important role in the pathogenesis of hepatic lipidosis and may be effective in increasing the severity of the disease.

 Lipidosis is generally defined as the accumulation of triglycerides in limited droplets within the cytoplasm of parenchymal cells. Lipidosis occurs due to toxic, chemical, infectious, and metabolic causes. This study aimed to reveal the local 8-hydroxy-2-deoxyguanosine 8-OHdG and 4-hydroxynonenal 4-HNE expressions immunohistochemically according to the severity of the disease in sheep with hepatic lipidosis. The study material consisted of a total of 30 male sheep livers, including 6 healthy and 24 with hepatic lipidosis. After the liver samples were fixed in 10 formaldehyde solution, they underwent routine tissue processing to obtain paraffin blocks. Sections taken from the paraffin blocks were then subjected to Hematoxylin-Eosin H-E and immunohistochemical staining. Microscopically, control group liver samples showed normal histology. In livers with hepatic lipidosis, sharp-edged vacuoles of various sizes were detected in hepatocytes, and cell nuclei were pushed to the periphery. Additionally, focal hemorrhage and congestion, inflammatory cell infiltration in the portal area, bile duct proliferation, and connective tissue cells were observed. Microscopically, hepatic lipidosis cases were divided into two groups as moderate and severe based on the distribution of vacuoles in the section. In the immunohistochemical examination, 8-OHdG and 4-HNE expressions significantly increased in hepatic lipidosis cases compared to the control group p0.001. More intense immunoreactivity was detected especially in cases where disease severity increased p0.001. These results indicate that 8-OHdG and 4-HNE proteins play an important role in the pathogenesis of hepatic lipidosis and may be effective in increasing the severity of the disease.

 Lipidosis is generally defined as the accumulation of triglycerides in limited droplets within the cytoplasm of parenchymal cells. Lipidosis occurs due to toxic, chemical, infectious, and metabolic causes. This study aimed to reveal the local 8-hydroxy-2-deoxyguanosine 8-OHdG and 4-hydroxynonenal 4-HNE expressions immunohistochemically according to the severity of the disease in sheep with hepatic lipidosis. The study material consisted of a total of 30 male sheep livers, including 6 healthy and 24 with hepatic lipidosis. After the liver samples were fixed in 10 formaldehyde solution, they underwent routine tissue processing to obtain paraffin blocks. Sections taken from the paraffin blocks were then subjected to Hematoxylin-Eosin H-E and immunohistochemical staining. Microscopically, control group liver samples showed normal histology. In livers with hepatic lipidosis, sharp-edged vacuoles of various sizes were detected in hepatocytes, and cell nuclei were pushed to the periphery. Additionally, focal hemorrhage and congestion, inflammatory cell infiltration in the portal area, bile duct proliferation, and connective tissue cells were observed. Microscopically, hepatic lipidosis cases were divided into two groups as moderate and severe based on the distribution of vacuoles in the section. In the immunohistochemical examination, 8-OHdG and 4-HNE expressions significantly increased in hepatic lipidosis cases compared to the control group p0.001. More intense immunoreactivity was detected especially in cases where disease severity increased p0.001. These results indicate that 8-OHdG and 4-HNE proteins play an important role in the pathogenesis of hepatic lipidosis and may be effective in increasing the severity of the disease.

 Lipidosis is generally defined as the accumulation of triglycerides in limited droplets within the cytoplasm of parenchymal cells. Lipidosis occurs due to toxic, chemical, infectious, and metabolic causes. This study aimed to reveal the local 8-hydroxy-2-deoxyguanosine 8-OHdG and 4-hydroxynonenal 4-HNE expressions immunohistochemically according to the severity of the disease in sheep with hepatic lipidosis. The study material consisted of a total of 30 male sheep livers, including 6 healthy and 24 with hepatic lipidosis. After the liver samples were fixed in 10 formaldehyde solution, they underwent routine tissue processing to obtain paraffin blocks. Sections taken from the paraffin blocks were then subjected to Hematoxylin-Eosin H-E and immunohistochemical staining. Microscopically, control group liver samples showed normal histology. In livers with hepatic lipidosis, sharp-edged vacuoles of various sizes were detected in hepatocytes, and cell nuclei were pushed to the periphery. Additionally, focal hemorrhage and congestion, inflammatory cell infiltration in the portal area, bile duct proliferation, and connective tissue cells were observed. Microscopically, hepatic lipidosis cases were divided into two groups as moderate and severe based on the distribution of vacuoles in the section. In the immunohistochemical examination, 8-OHdG and 4-HNE expressions significantly increased in hepatic lipidosis cases compared to the control group p0.001. More intense immunoreactivity was detected especially in cases where disease severity increased p0.001. These results indicate that 8-OHdG and 4-HNE proteins play an important role in the pathogenesis of hepatic lipidosis and may be effective in increasing the severity of the disease.

Makale detayları

Determination of 8-OHdG and 4-HNE Expressions in Sheep with Hepatic Lipidosis by Immunohistochemical Method

Yazarlar:

Özhan Karataş | Gökhan Akçakavak | Murat Eren | Zeynep Çelik | Ayşenur Tural | Osman Dağar | Osman Doğan | Bahadır Kılınç | Mehmet Tuzcu

Yayıncı:

Kyrgyz-Turkish Manas University

Ana Başlıklar:

Veteriner Patoloji

Dergi:

Manas Journal of Agriculture Veterinary and Life Sciences Vol.15 Issue 1, pp.9-15

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352125

pISSN:

1694-7932

8-hydroxy-2

4-Hydroxynonenal

hepatic lipidosis

sheep

Sayı

Makale detayları

Determination of 8-OHdG and 4-HNE Expressions in Sheep with Hepatic Lipidosis by Immunohistochemical Method

Yazarlar: Özhan Karataş | Gökhan Akçakavak | Murat Eren | Zeynep Çelik | Ayşenur Tural | Osman Dağar | Osman Doğan | Bahadır Kılınç | Mehmet Tuzcu

Yayıncı: Kyrgyz-Turkish Manas University

Ana Başlıklar: Veteriner Patoloji

Dergi: Manas Journal of Agriculture Veterinary and Life Sciences Vol.15 Issue 1, pp.9-15

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001352125

pISSN: 1694-7932

8-hydroxy-2

4-Hydroxynonenal

hepatic lipidosis

sheep

Sayı

Yazarlar:

Özhan Karataş | Gökhan Akçakavak | Murat Eren | Zeynep Çelik | Ayşenur Tural | Osman Dağar | Osman Doğan | Bahadır Kılınç | Mehmet Tuzcu

Yayıncı:

Kyrgyz-Turkish Manas University

Ana Başlıklar:

Veteriner Patoloji

Dergi:

Manas Journal of Agriculture Veterinary and Life Sciences Vol.15 Issue 1, pp.9-15

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001352125

pISSN:

1694-7932