Clonal comparison of Acinetobacter strains isolated from intensive care patients and the intensive care unit environment

Esra KIRKGÖZ; Yasemin ZER

Makale detayları

Clonal comparison of Acinetobacter strains isolated from intensive care patients and the intensive care unit environment

Yazarlar:

Esra KIRKGÖZ | Yasemin ZER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2014 Vol.44 Issue 4, pp.643-648

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000753175

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

Acinetobacter baumannii is one of the most commonly encountered microorganisms in nosocomial infections. It is thought that strains found in the environment can be a source for contamination of patients by Acinetobacter strains that are resistant to environmental conditions. This study was carried out to compare Acinetobacter strains isolated from a variety of nonviable environments and from patients in intensive care units (ICUs), and to explore whether environmental areas may be a source for bacterial contamination.    Materials and methods: We studied A. baumannii strains isolated from ICU patients. When A. baumannii was isolated from the clinical sample of a patient, swab samples were collected from various areas in the ICU. VITEK 2 (bioMérieux, France) was used for the identification of A. baumannii strains and for antibiotic sensitivity tests. DNA fingerprinting analysis was performed with the repetitive sequence-based polymerase chain reaction (rep-PCR) method using a DiversiLab device (bioMérieux).   Results: During the study, a total of 100 A. baumannii strains including 92 samples from 61 patients and 8 samples from the environment were isolated. All of the isolated strains were found to have multiresistance to antibiotics. DNA fingerprinting results showed that 7 of the 8 strains isolated from the environment were identical to many strains isolated from the patients. The greatest similarity between samples was found for 1 A. baumannii strain isolated from a computer keyboard, which was identical to the bacterium isolated from 17 other samples.   Conclusion: A high level of similarity was found between strains isolated from the environment and patients, suggesting deficiencies in implementation of infection control measures.

 

Acinetobacter baumannii is one of the most commonly encountered microorganisms in nosocomial infections. It is thought that strains found in the environment can be a source for contamination of patients by Acinetobacter strains that are resistant to environmental conditions. This study was carried out to compare Acinetobacter strains isolated from a variety of nonviable environments and from patients in intensive care units (ICUs), and to explore whether environmental areas may be a source for bacterial contamination.    Materials and methods: We studied A. baumannii strains isolated from ICU patients. When A. baumannii was isolated from the clinical sample of a patient, swab samples were collected from various areas in the ICU. VITEK 2 (bioMérieux, France) was used for the identification of A. baumannii strains and for antibiotic sensitivity tests. DNA fingerprinting analysis was performed with the repetitive sequence-based polymerase chain reaction (rep-PCR) method using a DiversiLab device (bioMérieux).   Results: During the study, a total of 100 A. baumannii strains including 92 samples from 61 patients and 8 samples from the environment were isolated. All of the isolated strains were found to have multiresistance to antibiotics. DNA fingerprinting results showed that 7 of the 8 strains isolated from the environment were identical to many strains isolated from the patients. The greatest similarity between samples was found for 1 A. baumannii strain isolated from a computer keyboard, which was identical to the bacterium isolated from 17 other samples.   Conclusion: A high level of similarity was found between strains isolated from the environment and patients, suggesting deficiencies in implementation of infection control measures.

Makale detayları

Clonal comparison of Acinetobacter strains isolated from intensive care patients and the intensive care unit environment

Yazarlar:

Esra KIRKGÖZ | Yasemin ZER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2014 Vol.44 Issue 4, pp.643-648

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000753175

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Acinetobacter baumannii

source

rep-PCR

nosocomial infection

Sayı

Makale detayları

Clonal comparison of Acinetobacter strains isolated from intensive care patients and the intensive care unit environment

Yazarlar: Esra KIRKGÖZ | Yasemin ZER

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2014 Vol.44 Issue 4, pp.643-648

Belge Tipi: Makale

Yayın Tarihi: 5/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000753175

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Acinetobacter baumannii

source

rep-PCR

nosocomial infection

Sayı

Yazarlar:

Esra KIRKGÖZ | Yasemin ZER

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2014 Vol.44 Issue 4, pp.643-648

Belge Tipi:

Makale

Yayın Tarihi:

5/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000753175

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144