Investigation of the Potential Antioxidant and Antidiabetic Effects of Cotinus coggygria Scop.

Alperen Canpolat; Hafize Yuca; Bilge Aydın; Zuhal Güvenalp

Makale detayları

Investigation of the Potential Antioxidant and Antidiabetic Effects of Cotinus coggygria Scop.

Yazarlar:

Alperen Canpolat | Hafize Yuca | Bilge Aydın | Zuhal Güvenalp

Yayıncı:

Ataturk University

Ana Başlıklar:

Farmakognozi | Temel Farmakoloji | Tıbbi Farmakoloji

Dergi:

Current Research in Health Sciences Vol.1 Issue 3, pp.105-114

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330714

pISSN:

3023-6991

Anahtar kelimeler:

Özet:

 ABSTRACT Objective: The aim of this study is to evaluate and compare the total phenolic content, total flavonoid content, antioxidant activities ABTS and DPPH scavenging effects, and α-glucosidase inhibition effects of different parts of Cotinus coggygria Scop. green leaves, red leaves, branches, and the heartwood collected at different times. Methods: The total phenolic content of the extracts was determined using the method developed by Folin and Denis and modified by Singleton. The total flavonoid content was determined according to the method developed by Larkina and colleagues. To test the antioxidant capacities of the extracts, DPPH and ABTS scavenging capacity assays were conducted; and to test their antidiabetic effects, α-glucosidase enzyme inhibition assay was performed. Results: It was found that the heartwood extract had the highest values in terms of total phenolic content and total flavonoid content. In the antioxidant activity assays, similar to the results of the total phenolic and total flavonoid content determinations, the heartwood extract also showed the highest content. However, it was observed that all other extracts also exhibited high antioxidant activity, especially against the ABTS. In the α-glucosidase inhibition assay conducted to examine the antidiabetic effects of the extracts, it was found that, in addition to red and green leaves, other extracts also possessed antidiabetic activity. Conclusion: The antioxidant and antidiabetic effects of extracts obtained from different parts of C. coggygria, a species used in traditional medicine for its various therapeutic effects, have been scientifically evaluated, proven, and compared. Key Words: α-glucosidase inhibition, ABTS, Cotinus coggygria Scop., DPPH, total flavonoid content, total phenolic content.

 ABSTRACT Objective: The aim of this study is to evaluate and compare the total phenolic content, total flavonoid content, antioxidant activities ABTS and DPPH scavenging effects, and α-glucosidase inhibition effects of different parts of Cotinus coggygria Scop. green leaves, red leaves, branches, and the heartwood collected at different times. Methods: The total phenolic content of the extracts was determined using the method developed by Folin and Denis and modified by Singleton. The total flavonoid content was determined according to the method developed by Larkina and colleagues. To test the antioxidant capacities of the extracts, DPPH and ABTS scavenging capacity assays were conducted; and to test their antidiabetic effects, α-glucosidase enzyme inhibition assay was performed. Results: It was found that the heartwood extract had the highest values in terms of total phenolic content and total flavonoid content. In the antioxidant activity assays, similar to the results of the total phenolic and total flavonoid content determinations, the heartwood extract also showed the highest content. However, it was observed that all other extracts also exhibited high antioxidant activity, especially against the ABTS. In the α-glucosidase inhibition assay conducted to examine the antidiabetic effects of the extracts, it was found that, in addition to red and green leaves, other extracts also possessed antidiabetic activity. Conclusion: The antioxidant and antidiabetic effects of extracts obtained from different parts of C. coggygria, a species used in traditional medicine for its various therapeutic effects, have been scientifically evaluated, proven, and compared. Key Words: α-glucosidase inhibition, ABTS, Cotinus coggygria Scop., DPPH, total flavonoid content, total phenolic content.

 ABSTRACT Objective: The aim of this study is to evaluate and compare the total phenolic content, total flavonoid content, antioxidant activities ABTS and DPPH scavenging effects, and α-glucosidase inhibition effects of different parts of Cotinus coggygria Scop. green leaves, red leaves, branches, and the heartwood collected at different times. Methods: The total phenolic content of the extracts was determined using the method developed by Folin and Denis and modified by Singleton. The total flavonoid content was determined according to the method developed by Larkina and colleagues. To test the antioxidant capacities of the extracts, DPPH and ABTS scavenging capacity assays were conducted; and to test their antidiabetic effects, α-glucosidase enzyme inhibition assay was performed. Results: It was found that the heartwood extract had the highest values in terms of total phenolic content and total flavonoid content. In the antioxidant activity assays, similar to the results of the total phenolic and total flavonoid content determinations, the heartwood extract also showed the highest content. However, it was observed that all other extracts also exhibited high antioxidant activity, especially against the ABTS. In the α-glucosidase inhibition assay conducted to examine the antidiabetic effects of the extracts, it was found that, in addition to red and green leaves, other extracts also possessed antidiabetic activity. Conclusion: The antioxidant and antidiabetic effects of extracts obtained from different parts of C. coggygria, a species used in traditional medicine for its various therapeutic effects, have been scientifically evaluated, proven, and compared. Key Words: α-glucosidase inhibition, ABTS, Cotinus coggygria Scop., DPPH, total flavonoid content, total phenolic content.

 ÖZ Amaç: Cotinus coggygria Scop. türünün farklı zamanlarda toplanan farklı kısımlarının yeşil renkli yapraklar, kırmızı renkli yapraklar, ince dallar ve gövde iç kısmı toplam fenolik madde, toplam flavonoit madde, antioksidan ABTS ve DPPH süpürücü etki ve α-glukozidaz inhibisyon etkileri açısından değerlendirilmesi ve karşılaştırılması bu çalışmanın amacını oluşturmaktadır. Yöntemler: Ekstrelerin toplam fenolik madde miktarı Folin ve Denis tarafından geliştirilen, Singleton tarafından modifiye edilen yöntem kullanılarak tayin edilmiştir. Toplam flavonoit madde miktarı ise Larkina ve arkadaşları tarafından geliştirilen yönteme göre tayin edilmiştir. Ekstrelerin antioksidan kapasitelerinin test edilmesi amacıyla DPPH ve ABTS süpürücü kapasite tayini deneyleri; antidiyabetik etkilerinin test edilmesi amacıyla α-glukozidaz enzim inhibisyonu deneyleri yapılmıştır. Bulgular: Toplam fenolik madde, toplam flavonoit madde bakımından en yüksek değerlere gövde ekstresinin sahip olduğu bulunmuştur. Antioksidan aktivite deneylerinde de toplam fenolik madde ve toplam flavonoit madde miktar tayini deneylerinin sonuçlarıyla benzer şekilde en yüksek etkiye gövde ekstresinin sahip olduğu bulunmuştur. Bununla beraber diğer bütün ekstrelerin de özellikle ABTSye karşı yüksek antioksidan etki gösterdiği gözlenmiştir. Ekstrelerin antidiyabetik etkilerinin incelenmesi amacıyla yapılan α-glukozidaz inhibisyonu deneylerinde ise başta kırmızı ve yeşil yaprak olmak üzere diğer ekstrelerin de antidiyabetik etkiye sahip olduğu bulunmuştur. Sonuç: Halk arasında çeşitli tıbbi etkilerinden dolayı kullanılan C. coggygria türünün farklı kısımlarından elde edilen ekstrelerin antioksidan ve antidiyabetik etkileri bilimsel olarak değerlendirilmiş, kanıtlanmış ve karşılaştırılmıştır.

Makale detayları

Investigation of the Potential Antioxidant and Antidiabetic Effects of Cotinus coggygria Scop.

Yazarlar:

Alperen Canpolat | Hafize Yuca | Bilge Aydın | Zuhal Güvenalp

Yayıncı:

Ataturk University

Ana Başlıklar:

Farmakognozi | Temel Farmakoloji | Tıbbi Farmakoloji

Dergi:

Current Research in Health Sciences Vol.1 Issue 3, pp.105-114

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330714

pISSN:

3023-6991

α-glucosidase inhibition

ABTS•+

Cotinus coggygria Scop.

DPPH•

total flavonoid content

total phenolic contentα-glukozidaz inhibisyonu

ABTS•+

Cotinus coggygria Scop.

DPPH•

toplam fenolik içerik

toplam flavonoit içerik

Sayı

Makale detayları

Yazarlar: Alperen Canpolat | Hafize Yuca | Bilge Aydın | Zuhal Güvenalp

Yayıncı: Ataturk University

Ana Başlıklar: Farmakognozi | Temel Farmakoloji | Tıbbi Farmakoloji

Dergi: Current Research in Health Sciences Vol.1 Issue 3, pp.105-114

Belge Tipi: Makale

Yayın Tarihi: 10/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001330714

pISSN: 3023-6991

Sayı

Yazarlar:

Alperen Canpolat | Hafize Yuca | Bilge Aydın | Zuhal Güvenalp

Yayıncı:

Ataturk University

Ana Başlıklar:

Farmakognozi | Temel Farmakoloji | Tıbbi Farmakoloji

Dergi:

Current Research in Health Sciences Vol.1 Issue 3, pp.105-114

Belge Tipi:

Makale

Yayın Tarihi:

10/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001330714

pISSN:

3023-6991