Prognostic value of oocyte denudation on fertilization and embryo quality in patients undergoing intracytoplasmic sperm injection

Berrin Yangın; Bülent Ayas; Davut Güven; Leman Tomak; Aysın Pınar Türkmen

Makale detayları

Prognostic value of oocyte denudation on fertilization and embryo quality in patients undergoing intracytoplasmic sperm injection

Yazarlar:

Berrin Yangın | Bülent Ayas | Davut Güven | Leman Tomak | Aysın Pınar Türkmen

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Histology and Embryology

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 4, pp.778-782

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001338260

pISSN:

1309-4483

Anahtar kelimeler:

Özet:

 A retrospective analysis of data obtained from 2034 oocytes collected from 157 patients with unexplained infertility was performed. Two groups were planned according to the diameters of flexible polycarbonate pipettes used in denudation. Group 1: Partial denudation of oocytes using a 140µm diameter pipette and Group 2: Complete denudation of oocytes using a 135µm diameter pipette. The data obtained were evaluated using statistical methods. It was aimed to investigate the possible effects of denudation on oocyte maturation, oocyte degeneration rate, fertilization rate, embryo quality, embryo development rate, abortion and live birth rates. After partial and complete denudation, there was no statistically significant difference between Group 1 and Group 2 in terms of oocyte maturation, normal fertilization rate, early embryonal development, embryo transfer numbers, blastocyst stage embryo numbers and morphology. In the third day embryos obtained from Group 2 oocytes, the rate of blastomeres incompatible with the day of development, the rate of degeneration among oocytes and the number of patients whose pregnancies ended due to pathologic reasons were found to be high p0.036, p0.037, p0.035. Live birth rate was higher in pregnancies developing from group 1 oocytes p0.039. It was concluded that granulosa cells left around the oocyte by pipettes with standardized diameter opening during denudation increased the success of live birth.

 A retrospective analysis of data obtained from 2034 oocytes collected from 157 patients with unexplained infertility was performed. Two groups were planned according to the diameters of flexible polycarbonate pipettes used in denudation. Group 1: Partial denudation of oocytes using a 140µm diameter pipette and Group 2: Complete denudation of oocytes using a 135µm diameter pipette. The data obtained were evaluated using statistical methods. It was aimed to investigate the possible effects of denudation on oocyte maturation, oocyte degeneration rate, fertilization rate, embryo quality, embryo development rate, abortion and live birth rates. After partial and complete denudation, there was no statistically significant difference between Group 1 and Group 2 in terms of oocyte maturation, normal fertilization rate, early embryonal development, embryo transfer numbers, blastocyst stage embryo numbers and morphology. In the third day embryos obtained from Group 2 oocytes, the rate of blastomeres incompatible with the day of development, the rate of degeneration among oocytes and the number of patients whose pregnancies ended due to pathologic reasons were found to be high p0.036, p0.037, p0.035. Live birth rate was higher in pregnancies developing from group 1 oocytes p0.039. It was concluded that granulosa cells left around the oocyte by pipettes with standardized diameter opening during denudation increased the success of live birth.

 A retrospective analysis of data obtained from 2034 oocytes collected from 157 patients with unexplained infertility was performed. Two groups were planned according to the diameters of flexible polycarbonate pipettes used in denudation. Group 1: Partial denudation of oocytes using a 140µm diameter pipette and Group 2: Complete denudation of oocytes using a 135µm diameter pipette. The data obtained were evaluated using statistical methods. It was aimed to investigate the possible effects of denudation on oocyte maturation, oocyte degeneration rate, fertilization rate, embryo quality, embryo development rate, abortion and live birth rates. After partial and complete denudation, there was no statistically significant difference between Group 1 and Group 2 in terms of oocyte maturation, normal fertilization rate, early embryonal development, embryo transfer numbers, blastocyst stage embryo numbers and morphology. In the third day embryos obtained from Group 2 oocytes, the rate of blastomeres incompatible with the day of development, the rate of degeneration among oocytes and the number of patients whose pregnancies ended due to pathologic reasons were found to be high p0.036, p0.037, p0.035. Live birth rate was higher in pregnancies developing from group 1 oocytes p0.039. It was concluded that granulosa cells left around the oocyte by pipettes with standardized diameter opening during denudation increased the success of live birth.

 A retrospective analysis of data obtained from 2034 oocytes collected from 157 patients with unexplained infertility was performed. Two groups were planned according to the diameters of flexible polycarbonate pipettes used in denudation. Group 1: Partial denudation of oocytes using a 140µm diameter pipette and Group 2: Complete denudation of oocytes using a 135µm diameter pipette. The data obtained were evaluated using statistical methods. It was aimed to investigate the possible effects of denudation on oocyte maturation, oocyte degeneration rate, fertilization rate, embryo quality, embryo development rate, abortion and live birth rates. After partial and complete denudation, there was no statistically significant difference between Group 1 and Group 2 in terms of oocyte maturation, normal fertilization rate, early embryonal development, embryo transfer numbers, blastocyst stage embryo numbers and morphology. In the third day embryos obtained from Group 2 oocytes, the rate of blastomeres incompatible with the day of development, the rate of degeneration among oocytes and the number of patients whose pregnancies ended due to pathologic reasons were found to be high p0.036, p0.037, p0.035. Live birth rate was higher in pregnancies developing from group 1 oocytes p0.039. It was concluded that granulosa cells left around the oocyte by pipettes with standardized diameter opening during denudation increased the success of live birth.

Makale detayları

Prognostic value of oocyte denudation on fertilization and embryo quality in patients undergoing intracytoplasmic sperm injection

Yazarlar:

Berrin Yangın | Bülent Ayas | Davut Güven | Leman Tomak | Aysın Pınar Türkmen

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Histology and Embryology

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 4, pp.778-782

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001338260

pISSN:

1309-4483

Granulosa cells

cumulus

intracytoplasmic sperm injection

Sayı

Makale detayları

Prognostic value of oocyte denudation on fertilization and embryo quality in patients undergoing intracytoplasmic sperm injection

Yazarlar: Berrin Yangın | Bülent Ayas | Davut Güven | Leman Tomak | Aysın Pınar Türkmen

Yayıncı: Ondokuz Mayıs University

Ana Başlıklar: Histology and Embryology

Dergi: Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 4, pp.778-782

Belge Tipi: Makale

Yayın Tarihi: 12/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001338260

pISSN: 1309-4483

Granulosa cells

cumulus

intracytoplasmic sperm injection

Sayı

Yazarlar:

Berrin Yangın | Bülent Ayas | Davut Güven | Leman Tomak | Aysın Pınar Türkmen

Yayıncı:

Ondokuz Mayıs University

Ana Başlıklar:

Histology and Embryology

Dergi:

Deneysel ve Klinik Tıp Dergisi Vol.41 Issue 4, pp.778-782

Belge Tipi:

Makale

Yayın Tarihi:

12/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001338260

pISSN:

1309-4483