In vitro characterization of alginate-chitosan hydrogels prepared with pH modification

Sevinç Şahbaz; Suna Özbaş

Makale detayları

In vitro characterization of alginate-chitosan hydrogels prepared with pH modification

Yazarlar:

Sevinç Şahbaz | Suna Özbaş

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 6, pp.2405-2415

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346305

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 Hydrogels are biomaterials frequently used as carrier systems for wound care, tissue engineering, and local drug applications. Our study aimed to prepare and characterize hydrogels using chitosan and sodium alginate through pH modification. Mechanical properties hardness, adhesiveness, cohesiveness, compressibility, and elasticity, viscosity, surface morphology, and cytotoxicity of polyion complex hydrogels containing different ratios of sodium alginate and chitosan were investigated. Mechanical properties were determined with a texture analyzer. The viscosity values of hydrogels varied between 12235 and 40743.3 cP. Hydrogel samples absorbed water up to 1000  1400 of their weight. The effect of pH and polymer concentration on the structural and mechanical properties of alginate-chitosan hydrogels was demonstrated. The findings showed that the decrease in pH improved the mechanical properties of alginate-chitosan hydrogels and increased the viscosity. The concentrations of chitosan and sodium alginate also altered the properties of hydrogels depending on pH. The formulation H5, which had the highest polymer ratio 3 and lower pH, showed the highest hardness 0.285  0.018 N, adhesiveness 0.824  0.042 N. s, and compressibility 1.3340.020 N.mm values. The results showed that the prepared alginate-chitosan hydrogels are not cytotoxic cell viability of over 80 in L-929 cell line and safe for use in living organisms.

 Hydrogels are biomaterials frequently used as carrier systems for wound care, tissue engineering, and local drug applications. Our study aimed to prepare and characterize hydrogels using chitosan and sodium alginate through pH modification. Mechanical properties hardness, adhesiveness, cohesiveness, compressibility, and elasticity, viscosity, surface morphology, and cytotoxicity of polyion complex hydrogels containing different ratios of sodium alginate and chitosan were investigated. Mechanical properties were determined with a texture analyzer. The viscosity values of hydrogels varied between 12235 and 40743.3 cP. Hydrogel samples absorbed water up to 1000  1400 of their weight. The effect of pH and polymer concentration on the structural and mechanical properties of alginate-chitosan hydrogels was demonstrated. The findings showed that the decrease in pH improved the mechanical properties of alginate-chitosan hydrogels and increased the viscosity. The concentrations of chitosan and sodium alginate also altered the properties of hydrogels depending on pH. The formulation H5, which had the highest polymer ratio 3 and lower pH, showed the highest hardness 0.285  0.018 N, adhesiveness 0.824  0.042 N. s, and compressibility 1.3340.020 N.mm values. The results showed that the prepared alginate-chitosan hydrogels are not cytotoxic cell viability of over 80 in L-929 cell line and safe for use in living organisms.

 Hydrogels are biomaterials frequently used as carrier systems for wound care, tissue engineering, and local drug applications. Our study aimed to prepare and characterize hydrogels using chitosan and sodium alginate through pH modification. Mechanical properties hardness, adhesiveness, cohesiveness, compressibility, and elasticity, viscosity, surface morphology, and cytotoxicity of polyion complex hydrogels containing different ratios of sodium alginate and chitosan were investigated. Mechanical properties were determined with a texture analyzer. The viscosity values of hydrogels varied between 12235 and 40743.3 cP. Hydrogel samples absorbed water up to 1000  1400 of their weight. The effect of pH and polymer concentration on the structural and mechanical properties of alginate-chitosan hydrogels was demonstrated. The findings showed that the decrease in pH improved the mechanical properties of alginate-chitosan hydrogels and increased the viscosity. The concentrations of chitosan and sodium alginate also altered the properties of hydrogels depending on pH. The formulation H5, which had the highest polymer ratio 3 and lower pH, showed the highest hardness 0.285  0.018 N, adhesiveness 0.824  0.042 N. s, and compressibility 1.3340.020 N.mm values. The results showed that the prepared alginate-chitosan hydrogels are not cytotoxic cell viability of over 80 in L-929 cell line and safe for use in living organisms.

 Hydrogels are biomaterials frequently used as carrier systems for wound care, tissue engineering, and local drug applications. Our study aimed to prepare and characterize hydrogels using chitosan and sodium alginate through pH modification. Mechanical properties hardness, adhesiveness, cohesiveness, compressibility, and elasticity, viscosity, surface morphology, and cytotoxicity of polyion complex hydrogels containing different ratios of sodium alginate and chitosan were investigated. Mechanical properties were determined with a texture analyzer. The viscosity values of hydrogels varied between 12235 and 40743.3 cP. Hydrogel samples absorbed water up to 1000  1400 of their weight. The effect of pH and polymer concentration on the structural and mechanical properties of alginate-chitosan hydrogels was demonstrated. The findings showed that the decrease in pH improved the mechanical properties of alginate-chitosan hydrogels and increased the viscosity. The concentrations of chitosan and sodium alginate also altered the properties of hydrogels depending on pH. The formulation H5, which had the highest polymer ratio 3 and lower pH, showed the highest hardness 0.285  0.018 N, adhesiveness 0.824  0.042 N. s, and compressibility 1.3340.020 N.mm values. The results showed that the prepared alginate-chitosan hydrogels are not cytotoxic cell viability of over 80 in L-929 cell line and safe for use in living organisms.

Makale detayları

In vitro characterization of alginate-chitosan hydrogels prepared with pH modification

Yazarlar:

Sevinç Şahbaz | Suna Özbaş

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 6, pp.2405-2415

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346305

pISSN:

2630-6344

chitosan

sodium alginate

hydrogel

TPA analysis

polyion complex

Sayı

Makale detayları

In vitro characterization of alginate-chitosan hydrogels prepared with pH modification

Yazarlar: Sevinç Şahbaz | Suna Özbaş

Yayıncı: Marmara University

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences (Other)

Dergi: Journal of Research in Pharmacy Vol.27 Issue 6, pp.2405-2415

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001346305

pISSN: 2630-6344

chitosan

sodium alginate

hydrogel

TPA analysis

polyion complex

Sayı

Yazarlar:

Sevinç Şahbaz | Suna Özbaş

Yayıncı:

Marmara University

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences (Other)

Dergi:

Journal of Research in Pharmacy Vol.27 Issue 6, pp.2405-2415

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001346305

pISSN:

2630-6344