Evaluation of the Effect of the Size of Ramalina fraxinea Based Ag NPs on the Antimicrobial Activity

N.A.

Makale detayları

Evaluation of the Effect of the Size of Ramalina fraxinea Based Ag NPs on the Antimicrobial Activity

Yazarlar:

N.A.

Yayıncı:

Erciyes University

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 1, pp.1-7

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287329

pISSN:

1304-7280

Anahtar kelimeler:

Özet:

 For the first time in this study, the effect of nanoparticles NP sizes on the antimicrobial activity of Ag NPs of different sizes obtained by the reaction of Ramalina fraxineaR. fraxinea extract and silver nitrate AgNO3 at different concentrations was evaluated. For this purpose, characteristic light absorption points, and charge of the surface were determined by ultraviolet-visible spectroscopy UV-Vis, and zeta potential, hydrodynamic diameters by dynamic light scattering DLS analysis, morphology, and diameters of NPs were determined by scanning electron microscope SEM analysis. The crystal structures of Ag NPs were confirmed by the X-ray diffraction XRD analysis map. The functional groups of the R. fraxinea extract, which plays an active role in the synthesis of Ag NP, were revealed by the peaks obtained by Fourier transform infrared spectroscopy FT-IR analysis. As a result of the characterization tests, the average diameter of Ag NPs increased from 14 nm to 48 nm with the increase in Ag NO3 concentration used in the synthesis of Ag NP from 10-3 M to 510-2 M. The antimicrobial activity of Ag NPs against Staphylococcus aureus, Escherichia coli, and Candida albicans strains was evaluated depending on the size of the NPs. The antimicrobial effect of small-sized Ag NPs against the studied strains was quite effective compared to large-sized Ag NPs. As a result, it is seen that Ag NPs synthesized by using R. fraxinea extract in different sizes with a biologically inexpensive and eco friendly method exhibit antimicrobial activity depending on the size. It is thought that the study data will be a guide for nanotechnology and biomedical field studies.

 For the first time in this study, the effect of nanoparticles NP sizes on the antimicrobial activity of Ag NPs of different sizes obtained by the reaction of Ramalina fraxineaR. fraxinea extract and silver nitrate AgNO3 at different concentrations was evaluated. For this purpose, characteristic light absorption points, and charge of the surface were determined by ultraviolet-visible spectroscopy UV-Vis, and zeta potential, hydrodynamic diameters by dynamic light scattering DLS analysis, morphology, and diameters of NPs were determined by scanning electron microscope SEM analysis. The crystal structures of Ag NPs were confirmed by the X-ray diffraction XRD analysis map. The functional groups of the R. fraxinea extract, which plays an active role in the synthesis of Ag NP, were revealed by the peaks obtained by Fourier transform infrared spectroscopy FT-IR analysis. As a result of the characterization tests, the average diameter of Ag NPs increased from 14 nm to 48 nm with the increase in Ag NO3 concentration used in the synthesis of Ag NP from 10-3 M to 510-2 M. The antimicrobial activity of Ag NPs against Staphylococcus aureus, Escherichia coli, and Candida albicans strains was evaluated depending on the size of the NPs. The antimicrobial effect of small-sized Ag NPs against the studied strains was quite effective compared to large-sized Ag NPs. As a result, it is seen that Ag NPs synthesized by using R. fraxinea extract in different sizes with a biologically inexpensive and eco friendly method exhibit antimicrobial activity depending on the size. It is thought that the study data will be a guide for nanotechnology and biomedical field studies.

 For the first time in this study, the effect of nanoparticles NP sizes on the antimicrobial activity of Ag NPs of different sizes obtained by the reaction of Ramalina fraxineaR. fraxinea extract and silver nitrate AgNO3 at different concentrations was evaluated. For this purpose, characteristic light absorption points, and charge of the surface were determined by ultraviolet-visible spectroscopy UV-Vis, and zeta potential, hydrodynamic diameters by dynamic light scattering DLS analysis, morphology, and diameters of NPs were determined by scanning electron microscope SEM analysis. The crystal structures of Ag NPs were confirmed by the X-ray diffraction XRD analysis map. The functional groups of the R. fraxinea extract, which plays an active role in the synthesis of Ag NP, were revealed by the peaks obtained by Fourier transform infrared spectroscopy FT-IR analysis. As a result of the characterization tests, the average diameter of Ag NPs increased from 14 nm to 48 nm with the increase in Ag NO3 concentration used in the synthesis of Ag NP from 10-3 M to 510-2 M. The antimicrobial activity of Ag NPs against Staphylococcus aureus, Escherichia coli, and Candida albicans strains was evaluated depending on the size of the NPs. The antimicrobial effect of small-sized Ag NPs against the studied strains was quite effective compared to large-sized Ag NPs. As a result, it is seen that Ag NPs synthesized by using R. fraxinea extract in different sizes with a biologically inexpensive and eco friendly method exhibit antimicrobial activity depending on the size. It is thought that the study data will be a guide for nanotechnology and biomedical field studies.

 Bu çalışmada ilk kez Ramalina fraxinea özütü ile farklı konsantrasyonlarda AgNO3 reaksiyonu sonucunda elde edilen farklı boyutlara sahip Ag NPlerin karakterizasyonları değerlendirilmiştir. Bu amaçla NPlerin UV-Vis. analizi ile karakteristik ışık emme noktaları, zeta testi ile yüzey yükleri DLS analizi ile hidrodinamik çapları, SEM analizi ile NPle- rin morfoloji ve çapları tespit edilmiştir. Ag NPlerin kristal yapıları XRD haritası ile doğrulanmıştır. Ag NPnin sentezinde aktif olarak rol oynayan R. fraxinea özütünün fonksiyonel grupları FT-IR analizi ile elde edilen pikler ile ortaya konmuş- tur. Yapılan karakterizasyon testleri sonucunda Ag NPnin sentezinde kullanılan Ag NO3 konsantrasyonunun artışı ile birlikte 10-3 M-510-2 M AgNPlerin ortalama çapı 14 nmden 48 nmye artış göstermiştir. Staphylococcus aureus, Esc- herichia coli ve Candida albicans suşlarına karşı Ag NPlerin antimikrobiyal etkinliği NPlerin boyutuna bağlı olarak de- ğerlendirilmiştir. Küçük boyuta sahip Ag NPlerin çalışılan suşlara karşı antimikrobiyal etkisi büyük boyuta sahip AgNPlere göre oldukça etkiliydi. Sonuç olarak R. fraxinea özütü ile biyolojik olarak ucuz etkili ve çevre dostu yöntemle farklı boyutlarda sentezlenen AgNPlerin boyuta bağlı olarak antimikrobiyal aktivite sergilediği görülmektedir. Çalışma verilerinin nanoteknoloji ve biyomedikal alan çalışmaları için yol gösterici olacağı düşünülmektedir.

Makale detayları

Evaluation of the Effect of the Size of Ramalina fraxinea Based Ag NPs on the Antimicrobial Activity

Yazarlar:

N.A.

Yayıncı:

Erciyes University

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 1, pp.1-7

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287329

pISSN:

1304-7280

Antimicrobial activity

biosynthesis

Ramalina fraxinea

silver nanoparticles

Antimikrobiyal aktivite

biyolojik sentez

Ramalina fraxinea

gümüş nanopartikülleri

Sayı

Makale detayları

Yazarlar: N.A.

Yayıncı: Erciyes University

Dergi: Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 1, pp.1-7

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001287329

pISSN: 1304-7280

Sayı

Yazarlar:

N.A.

Yayıncı:

Erciyes University

Dergi:

Erciyes Üniversitesi Veteriner Fakültesi Dergisi Vol.20 Issue 1, pp.1-7

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001287329

pISSN:

1304-7280