Identification of causative species in patients with cuteneous leishmaniasis in Diyarbakır by polymerase chain reaction (pcr)-restriction fragment lenght polymorphism (RFLP)

Nezahat AKPOLAT; İbrahim Halil YILDIRIM; Mutalip ÇİÇEK; Şebnem NERGİZ; Özgür EZİN; Nida ÖZCAN; Mehmet HARMAN

Makale detayları

Identification of causative species in patients with cuteneous leishmaniasis in Diyarbakır by polymerase chain reaction (pcr)-restriction fragment lenght polymorphism (RFLP)

Yazarlar:

Nezahat AKPOLAT | İbrahim Halil YILDIRIM | Mutalip ÇİÇEK | Şebnem NERGİZ | Özgür EZİN | Nida ÖZCAN | Mehmet HARMAN

Yayıncı:

Veysi AKPOLAT

Dergi:

International Archives of Medical Research 2014 Vol.6 Issue 2, pp.17-20

Belge Tipi:

Makale

Yayın Tarihi:

11/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000470337

Sıklık:

İki Yıllık

pISSN:

2146-6033

Özet:

 

Background: Leishmaniasis is a protozoan disease caused by more than twenty Leishmania species which
 are transmitted by infected phlebotomine sandflies to humans. Leishmaniasis include multiple clinical
 syndromes such as visceral , mucosal and cutaneous forms. Cutaneous Leishmaniasis (CL) is the most
 common and endemic form in southeastern Turkey.
 Purpose: CL can be caused by L. major, L. tropica, L. mexicana and L. amazonensis. Microscopy remains to
 be the standard diagnostic method because of its cheapness, ease of application and high
 specificity . Microscopic examination of smears, parasite culture and serological tests are performed for
 diagnosis. Recently several molecular methods, especially those based on the Polymerase chain reaction
 (PCR) have been developed for identification of Leishmania species.
 Method: In this study, 150 smear samples taken from patients with clinical findings of CL were studied by
 PCR- RFLP (restriction fragment length polymorphism)
 Results: Leishmania tropica was detected in 128 smear sample. L. tropica was found to be the most common
 species causing CL in southeastern Turkey.
 Conclusion: Identification of Leishmania species is important because different types may require different
 treatments. Determining the common species in the region may lead to develop treatment protocols.

Makale detayları

Identification of causative species in patients with cuteneous leishmaniasis in Diyarbakır by polymerase chain reaction (pcr)-restriction fragment lenght polymorphism (RFLP)

Yazarlar: Nezahat AKPOLAT | İbrahim Halil YILDIRIM | Mutalip ÇİÇEK | Şebnem NERGİZ | Özgür EZİN | Nida ÖZCAN | Mehmet HARMAN

Yayıncı: Veysi AKPOLAT

Dergi: International Archives of Medical Research 2014 Vol.6 Issue 2, pp.17-20

Belge Tipi: Makale

Yayın Tarihi: 11/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000470337

Sıklık: İki Yıllık

pISSN: 2146-6033

Sayı

Yazarlar:

Nezahat AKPOLAT | İbrahim Halil YILDIRIM | Mutalip ÇİÇEK | Şebnem NERGİZ | Özgür EZİN | Nida ÖZCAN | Mehmet HARMAN

Yayıncı:

Veysi AKPOLAT

Dergi:

International Archives of Medical Research 2014 Vol.6 Issue 2, pp.17-20

Belge Tipi:

Makale

Yayın Tarihi:

11/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000470337

Sıklık:

İki Yıllık

pISSN:

2146-6033