The phylogeny of Heterocapsa sp. (Dinophyceae) isolated from the south coast of Iran during a Cochlodinium polykrikoides bloom

Gilan ATTARAN-FARIMAN; Pegah JAVID

Makale detayları

The phylogeny of Heterocapsa sp. (Dinophyceae) isolated from the south coast of Iran during a Cochlodinium polykrikoides bloom

Yazarlar:

Gilan ATTARAN-FARIMAN | Pegah JAVID

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2013 Vol.37 Issue 4, pp.778-783

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000722690

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Özet:

 

The genus Heterocapsa Stein is a relatively small armoured dinoflagellate. Because it has few differences in morphological characteristics among its species, species identification is difficult. In this study, small Heterocapsa sp. cells associated with a dense bloom of Cochlodinium polykrikoides Margalef were collected from the south-east coast of Iran. While Heterocapsa sp. was isolated, C. polykrikoides could not be isolated by single cell or serial dilution methods. Thus, a unialgal strain of Heterocapsa sp. was used for molecular analysis and species identification. In order to carry out phylogenetic analysis, rDNA was extracted and large subunit domains of D1-D3 were sequenced. Similar sequences of other geographical strains were selected from GenBank and compared with the Iranian species. The Iranian strain was aligned with Heterocapsa spp. Phylogenetic analysis of maximum likelihood and neighbor-joining demonstrated that Heterocapsa sp. does not form a monophyletic group. Morphological characteristics confirmed molecular results. This is the first record of Heterocapsa isolation from Iranian coastal waters.

 

The genus Heterocapsa Stein is a relatively small armoured dinoflagellate. Because it has few differences in morphological characteristics among its species, species identification is difficult. In this study, small Heterocapsa sp. cells associated with a dense bloom of Cochlodinium polykrikoides Margalef were collected from the south-east coast of Iran. While Heterocapsa sp. was isolated, C. polykrikoides could not be isolated by single cell or serial dilution methods. Thus, a unialgal strain of Heterocapsa sp. was used for molecular analysis and species identification. In order to carry out phylogenetic analysis, rDNA was extracted and large subunit domains of D1-D3 were sequenced. Similar sequences of other geographical strains were selected from GenBank and compared with the Iranian species. The Iranian strain was aligned with Heterocapsa spp. Phylogenetic analysis of maximum likelihood and neighbor-joining demonstrated that Heterocapsa sp. does not form a monophyletic group. Morphological characteristics confirmed molecular results. This is the first record of Heterocapsa isolation from Iranian coastal waters.

Makale detayları

The phylogeny of Heterocapsa sp. (Dinophyceae) isolated from the south coast of Iran during a Cochlodinium polykrikoides bloom

Yazarlar: Gilan ATTARAN-FARIMAN | Pegah JAVID

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2013 Vol.37 Issue 4, pp.778-783

Belge Tipi: Makale

Yayın Tarihi: 3/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000722690

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Sayı

Yazarlar:

Gilan ATTARAN-FARIMAN | Pegah JAVID

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2013 Vol.37 Issue 4, pp.778-783

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000722690

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X