The effects of p53 inhibition using pifithrin-&#945; on acute necrotizing pancreatitis in rats

Etem ALHAN; Akif CİNEL; Mehmet Cengiz ERÇİN; Serdar TÜRKYILMAZ; Birgül VANİZOR KURAL; Murat ALPER; Deniz FİLİNTE

Makale detayları

The effects of p53 inhibition using pifithrin-α on acute necrotizing pancreatitis in rats

Yazarlar:

Etem ALHAN | Akif CİNEL | Mehmet Cengiz ERÇİN | Serdar TÜRKYILMAZ | Birgül VANİZOR KURAL | Murat ALPER | Deniz FİLİNTE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2011 Vol.41 Issue 4, pp.673-683

Belge Tipi:

Makale

Yayın Tarihi:

3/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000754650

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

To investigate the effects of p53 inhibition using pifithrin-a (PFA) on acute necrotizing pancreatitis (ANP) induced by glycodeoxycholic acid in rats. The role of p53 during acute pancreatitis is unclear.   Materials and methods: Rats were divided into 4 groups, 2 of which were initially given only saline (the sham group) and 2 of which were given glycodeoxycholic acid in order to induce ANP. Each group was then identified according to treatment plan: sham+saline, sham+PFA, ANP+saline, or ANP+PFA. The study then investigated the extent of acinar cell injury, apoptosis, mortality, systemic cardiorespiratory variables, functional capillary density (FCD), renal/hepatic functions, and changes in some enzyme markers for pancreatic and lung tissue during ANP in rats.    Results: The induction of ANP resulted in a significant increase in mortality rate, pancreatic necrosis, apoptosis, serum activity of amylase, alanine aminotransferase (ALT), interleukin (IL)-6, lactate dehydrogenase (LDH) in bronchoalveolar lavage (BAL) fluid, serum concentration of urea, and tissue activity of myeloperoxidase (MPO) and malondialdehyde (MDA) in the pancreas and lung. In addition, ANP induction caused a significant decrease in concentrations of calcium, blood pressure, urine output, pO2, and functional capillary density (FCD). The use of PFA did not alter these changes.   Conclusion: PFA demonstrated no effect on the course of ANP in rats. Therefore, it has no value in the treatment of acute pancreatitis.

 

To investigate the effects of p53 inhibition using pifithrin-a (PFA) on acute necrotizing pancreatitis (ANP) induced by glycodeoxycholic acid in rats. The role of p53 during acute pancreatitis is unclear.   Materials and methods: Rats were divided into 4 groups, 2 of which were initially given only saline (the sham group) and 2 of which were given glycodeoxycholic acid in order to induce ANP. Each group was then identified according to treatment plan: sham+saline, sham+PFA, ANP+saline, or ANP+PFA. The study then investigated the extent of acinar cell injury, apoptosis, mortality, systemic cardiorespiratory variables, functional capillary density (FCD), renal/hepatic functions, and changes in some enzyme markers for pancreatic and lung tissue during ANP in rats.    Results: The induction of ANP resulted in a significant increase in mortality rate, pancreatic necrosis, apoptosis, serum activity of amylase, alanine aminotransferase (ALT), interleukin (IL)-6, lactate dehydrogenase (LDH) in bronchoalveolar lavage (BAL) fluid, serum concentration of urea, and tissue activity of myeloperoxidase (MPO) and malondialdehyde (MDA) in the pancreas and lung. In addition, ANP induction caused a significant decrease in concentrations of calcium, blood pressure, urine output, pO2, and functional capillary density (FCD). The use of PFA did not alter these changes.   Conclusion: PFA demonstrated no effect on the course of ANP in rats. Therefore, it has no value in the treatment of acute pancreatitis.

Makale detayları

The effects of p53 inhibition using pifithrin-α on acute necrotizing pancreatitis in rats

Yazarlar:

Etem ALHAN | Akif CİNEL | Mehmet Cengiz ERÇİN | Serdar TÜRKYILMAZ | Birgül VANİZOR KURAL | Murat ALPER | Deniz FİLİNTE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2011 Vol.41 Issue 4, pp.673-683

Belge Tipi:

Makale

Yayın Tarihi:

3/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000754650

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Key words: Acute pancreatitis

p53

pifithrin-a

Sayı

Makale detayları

The effects of p53 inhibition using pifithrin-&#945; on acute necrotizing pancreatitis in rats

Yazarlar: Etem ALHAN | Akif CİNEL | Mehmet Cengiz ERÇİN | Serdar TÜRKYILMAZ | Birgül VANİZOR KURAL | Murat ALPER | Deniz FİLİNTE

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 2011 Vol.41 Issue 4, pp.673-683

Belge Tipi: Makale

Yayın Tarihi: 3/2011

Belge Dili: Türkçe

Document ID (Org No.): 2000754650

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Key words: Acute pancreatitis

p53

pifithrin-a

Sayı

The effects of p53 inhibition using pifithrin-α on acute necrotizing pancreatitis in rats

Yazarlar:

Etem ALHAN | Akif CİNEL | Mehmet Cengiz ERÇİN | Serdar TÜRKYILMAZ | Birgül VANİZOR KURAL | Murat ALPER | Deniz FİLİNTE

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 2011 Vol.41 Issue 4, pp.673-683

Belge Tipi:

Makale

Yayın Tarihi:

3/2011

Belge Dili:

Türkçe

Document ID (Org No.):

2000754650

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144