Antisenescence activity of G9a inhibitor BIX01294 on human bone marrow mesenchymal stromal cells

Min-ji AHN; Sin-gu JEONG; Goang Won CHO

Makale detayları

Antisenescence activity of G9a inhibitor BIX01294 on human bone marrow mesenchymal stromal cells

Yazarlar:

Min-ji AHN | Sin-gu JEONG | Goang Won CHO

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 2, pp.443-451

Belge Tipi:

Makale

Yayın Tarihi:

3/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811736

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

Alterations in epigenomic patterns are associated with diverse physiological functions and pathological mechanisms causing aging-related diseases, such as degenerative disorders and cancers. We investigated the senescent effects of BIX01294, a G9a (histone methyltransferase) inhibitor, in human bone marrow mesenchymal stromal cells (hBM-MSCs). We determined the optimal dose and time of BIX01294 treatment in hBM-MSCs to be 1 μM and 12 h, respectively. Under these conditions, the expression of the antisenescent genes TERT, bFGF, VEGF, and Oct-4 increased, whereas the expression of the senescent factors p16, p21, and p53 decreased. The number of β-galactosidase-positive cells decreased significantly, and the rates of migration and cellular protection against oxidative damage increased in BIX01294-treated MSCs. These data indicate that an optimized dose of BIX01294 may improve the potency and senescence of stem cells, which may improve the efficacy of stem cell therapy.

Makale detayları

Antisenescence activity of G9a inhibitor BIX01294 on human bone marrow mesenchymal stromal cells

Yazarlar: Min-ji AHN | Sin-gu JEONG | Goang Won CHO

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2016 Vol.40 Issue 2, pp.443-451

Belge Tipi: Makale

Yayın Tarihi: 3/2016

Belge Dili: Türkçe

Document ID (Org No.): 2000811736

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Min-ji AHN | Sin-gu JEONG | Goang Won CHO

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 2, pp.443-451

Belge Tipi:

Makale

Yayın Tarihi:

3/2016

Belge Dili:

Türkçe

Document ID (Org No.):

2000811736

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152