Genotoxic potential of Cynanchum sarcomedium Meve & Liede coupled with its modulatory action on oxidative-stress–mediated genotoxicity by hydrogen peroxide

Neethu Kannan BHAGYANATHAN; John Ernest THOPPIL

Makale detayları

Genotoxic potential of Cynanchum sarcomedium Meve & Liede coupled with its modulatory action on oxidative-stress–mediated genotoxicity by hydrogen peroxide

Yazarlar:

Neethu Kannan BHAGYANATHAN | John Ernest THOPPIL

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 1, pp.120-129

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

İngilizce

Document ID (Org No.):

2000811760

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Özet:

 

The present study evaluated the genotoxic effect of aqueous extract of C. sarcomedium Meve and Liede and its protective role in chromosomal aberrations induced by 2% hydrogen peroxide (H2O2) on Allium cepa root meristem. Our results showed that the C. sarcomedium extract induced major chromosomal aberrations, viz., nuclear lesions, chromosome breaks, chromosome laggards, vagrance, stickiness, and bridges. These are irreversible in nature and indicate that the plant is a potent clastogen. Total chromosome aberrations were more frequent with increase in exposure time and concentrations of plant extract. For antigenotoxic screening, three different modes of treatment were used: pretreatment, posttreatment, and simultaneous treatment. The significant induction of a wide spectrum of chromosomal aberrations and the reduction in mitotic index effected by the aqueous extract reinforced the genotoxic potential of the plant. The modulatory effect of plant extract was expressed in terms of inhibition percentage and had an excellent effect in reversing chromosomal aberrations induced by H2O2. Dose-dependent results suggest the genotoxic and antigenotoxic effect of C. sarcomedium on onion root tips. These findings offer a useful basis for further investigations on C. sarcomedium, a shrub with a broad array of prospective therapeutic activities.

Makale detayları

Genotoxic potential of Cynanchum sarcomedium Meve & Liede coupled with its modulatory action on oxidative-stress–mediated genotoxicity by hydrogen peroxide

Yazarlar: Neethu Kannan BHAGYANATHAN | John Ernest THOPPIL

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2016 Vol.40 Issue 1, pp.120-129

Belge Tipi: Makale

Yayın Tarihi: 1/2016

Belge Dili: İngilizce

Document ID (Org No.): 2000811760

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Neethu Kannan BHAGYANATHAN | John Ernest THOPPIL

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2016 Vol.40 Issue 1, pp.120-129

Belge Tipi:

Makale

Yayın Tarihi:

1/2016

Belge Dili:

İngilizce

Document ID (Org No.):

2000811760

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152