Immunohistochemical Localization of Thymosin  b4 in Bovine Tissues  Ourania E. TSITSILONIS 1

Ayhan ULUBELEN

Makale detayları

Immunohistochemical Localization of Thymosin b4 in Bovine Tissues Ourania E. TSITSILONIS 1

Yazarlar:

Ayhan ULUBELEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 1999 Vol.29 Issue 3, pp.237-248

Belge Tipi:

Makale

Yayın Tarihi:

2/1999

Belge Dili:

Türkçe

Document ID (Org No.):

2000757981

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Anahtar kelimeler:

Özet:

 

The histochemical localization of  thymosin b4 was investigated for the first  time in several bovine tissues, using the  indirect immunofluorescent technique and  the newly applied alkaline phosphatase anti-alkaline  phosphatase (APAAP) antibody  method. A polyclonal antiserum raised  against the N-terminal fragment (1-14) of  thymosin b4, showing less than 0.1%  crossreactivity with the second b-thymosin in  calf tissues, thymosin b9, was used. Positive  reaction for thymosin b4 was observed in the  cytoplasm of all tissues studied, i.e., bovine  spleen, thymus, lung, liver, kidney, lymph  nodes and cardiac muscle. The highest  staining intensity was found in the  cytoplasmic area of spleen and thymus,  whereas in sections of muscle the reaction  was very weak. In the latter case a relatively  strong reaction was strictly located in the  sarcolemn region of the myofibrils.

 

The histochemical localization of  thymosin b4 was investigated for the first  time in several bovine tissues, using the  indirect immunofluorescent technique and  the newly applied alkaline phosphatase anti-alkaline  phosphatase (APAAP) antibody  method. A polyclonal antiserum raised  against the N-terminal fragment (1-14) of  thymosin b4, showing less than 0.1%  crossreactivity with the second b-thymosin in  calf tissues, thymosin b9, was used. Positive  reaction for thymosin b4 was observed in the  cytoplasm of all tissues studied, i.e., bovine  spleen, thymus, lung, liver, kidney, lymph  nodes and cardiac muscle. The highest  staining intensity was found in the  cytoplasmic area of spleen and thymus,  whereas in sections of muscle the reaction  was very weak. In the latter case a relatively  strong reaction was strictly located in the  sarcolemn region of the myofibrils.

Makale detayları

Immunohistochemical Localization of Thymosin b4 in Bovine Tissues Ourania E. TSITSILONIS 1

Yazarlar:

Ayhan ULUBELEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 1999 Vol.29 Issue 3, pp.237-248

Belge Tipi:

Makale

Yayın Tarihi:

2/1999

Belge Dili:

Türkçe

Document ID (Org No.):

2000757981

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Thymosin b4

bovine tissues

immunofluorescent technique.

Sayı

Makale detayları

Immunohistochemical Localization of Thymosin b4 in Bovine Tissues Ourania E. TSITSILONIS 1

Yazarlar: Ayhan ULUBELEN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Medical Sciences 1999 Vol.29 Issue 3, pp.237-248

Belge Tipi: Makale

Yayın Tarihi: 2/1999

Belge Dili: Türkçe

Document ID (Org No.): 2000757981

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Thymosin b4

bovine tissues

immunofluorescent technique.

Sayı

Yazarlar:

Ayhan ULUBELEN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Medical Sciences 1999 Vol.29 Issue 3, pp.237-248

Belge Tipi:

Makale

Yayın Tarihi:

2/1999

Belge Dili:

Türkçe

Document ID (Org No.):

2000757981

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144