Evaluation of amino acid clearances in post-transplant kidney patients stratified by eGFR

Soner Duman; Bulent Oktay; Mustafa Serteser

Makale detayları

Evaluation of amino acid clearances in post-transplant kidney patients stratified by eGFR

Yazarlar:

Soner Duman | Bulent Oktay | Mustafa Serteser

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Pharmacology and Therapeutics

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 4, pp.1265-1274

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347040

pISSN:

2630-6344

Anahtar kelimeler:

Özet:

 The human kidney has a prominent role in the metabolism of amino acids and the control of plasma concentrations. Treatment modalities, such as kidney transplantation, hemodialysis, etc., may alter amino acid concentrations. In the present study, we aimed to evaluate the effect of kidney transplantation on the amino acid profile and the relationship between creatinine clearance and amino acid clearances in post-transplant kidney patients. Serum and urine samples were obtained from 133 patients after kidney transplantation. Patients were divided into three groups based on eGFR. Amino acid profiles were performed by liquid chromatography coupled with tandem mass spectrometry, and clearances were calculated using the 24-hour creatinine clearance formula. The amino acid clearances between the studied groups differed significantly in 10 of the 27 amino acids. While 1-methyl-l-histidine, 3-methyl-l- histidine, Arginine, Cystine, Ethanolamine, Glycine, L-Histidine, Isoleucine, and Taurine significantly showed an upward trend, L-Proline especially revealed a downward trend. Amino acid clearances that showed the strongest correlation with creatinine clearance were 1-methyl-l-histidine, 3-methyl-l-histidine, and ethanolamine. However, Arginine and L-proline had a weak positive and negative linear relationship, respectively. This study showed that amino acid clearances varied in kidney transplant patients among the studied groups. Increased or decreased amino acid clearances are affected by the progression of renal dysfunction. These results give insight into kidney transplantation- associated modifications of amino acid metabolism.

 The human kidney has a prominent role in the metabolism of amino acids and the control of plasma concentrations. Treatment modalities, such as kidney transplantation, hemodialysis, etc., may alter amino acid concentrations. In the present study, we aimed to evaluate the effect of kidney transplantation on the amino acid profile and the relationship between creatinine clearance and amino acid clearances in post-transplant kidney patients. Serum and urine samples were obtained from 133 patients after kidney transplantation. Patients were divided into three groups based on eGFR. Amino acid profiles were performed by liquid chromatography coupled with tandem mass spectrometry, and clearances were calculated using the 24-hour creatinine clearance formula. The amino acid clearances between the studied groups differed significantly in 10 of the 27 amino acids. While 1-methyl-l-histidine, 3-methyl-l- histidine, Arginine, Cystine, Ethanolamine, Glycine, L-Histidine, Isoleucine, and Taurine significantly showed an upward trend, L-Proline especially revealed a downward trend. Amino acid clearances that showed the strongest correlation with creatinine clearance were 1-methyl-l-histidine, 3-methyl-l-histidine, and ethanolamine. However, Arginine and L-proline had a weak positive and negative linear relationship, respectively. This study showed that amino acid clearances varied in kidney transplant patients among the studied groups. Increased or decreased amino acid clearances are affected by the progression of renal dysfunction. These results give insight into kidney transplantation- associated modifications of amino acid metabolism.

 The human kidney has a prominent role in the metabolism of amino acids and the control of plasma concentrations. Treatment modalities, such as kidney transplantation, hemodialysis, etc., may alter amino acid concentrations. In the present study, we aimed to evaluate the effect of kidney transplantation on the amino acid profile and the relationship between creatinine clearance and amino acid clearances in post-transplant kidney patients. Serum and urine samples were obtained from 133 patients after kidney transplantation. Patients were divided into three groups based on eGFR. Amino acid profiles were performed by liquid chromatography coupled with tandem mass spectrometry, and clearances were calculated using the 24-hour creatinine clearance formula. The amino acid clearances between the studied groups differed significantly in 10 of the 27 amino acids. While 1-methyl-l-histidine, 3-methyl-l- histidine, Arginine, Cystine, Ethanolamine, Glycine, L-Histidine, Isoleucine, and Taurine significantly showed an upward trend, L-Proline especially revealed a downward trend. Amino acid clearances that showed the strongest correlation with creatinine clearance were 1-methyl-l-histidine, 3-methyl-l-histidine, and ethanolamine. However, Arginine and L-proline had a weak positive and negative linear relationship, respectively. This study showed that amino acid clearances varied in kidney transplant patients among the studied groups. Increased or decreased amino acid clearances are affected by the progression of renal dysfunction. These results give insight into kidney transplantation- associated modifications of amino acid metabolism.

 The human kidney has a prominent role in the metabolism of amino acids and the control of plasma concentrations. Treatment modalities, such as kidney transplantation, hemodialysis, etc., may alter amino acid concentrations. In the present study, we aimed to evaluate the effect of kidney transplantation on the amino acid profile and the relationship between creatinine clearance and amino acid clearances in post-transplant kidney patients. Serum and urine samples were obtained from 133 patients after kidney transplantation. Patients were divided into three groups based on eGFR. Amino acid profiles were performed by liquid chromatography coupled with tandem mass spectrometry, and clearances were calculated using the 24-hour creatinine clearance formula. The amino acid clearances between the studied groups differed significantly in 10 of the 27 amino acids. While 1-methyl-l-histidine, 3-methyl-l- histidine, Arginine, Cystine, Ethanolamine, Glycine, L-Histidine, Isoleucine, and Taurine significantly showed an upward trend, L-Proline especially revealed a downward trend. Amino acid clearances that showed the strongest correlation with creatinine clearance were 1-methyl-l-histidine, 3-methyl-l-histidine, and ethanolamine. However, Arginine and L-proline had a weak positive and negative linear relationship, respectively. This study showed that amino acid clearances varied in kidney transplant patients among the studied groups. Increased or decreased amino acid clearances are affected by the progression of renal dysfunction. These results give insight into kidney transplantation- associated modifications of amino acid metabolism.

Makale detayları

Evaluation of amino acid clearances in post-transplant kidney patients stratified by eGFR

Yazarlar:

Soner Duman | Bulent Oktay | Mustafa Serteser

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Pharmacology and Therapeutics

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 4, pp.1265-1274

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347040

pISSN:

2630-6344

Amino acids

kidney transplantation

amino acid clearance

estimated glomerular filtration rate

creatinine clearance.

Sayı

Makale detayları

Evaluation of amino acid clearances in post-transplant kidney patients stratified by eGFR

Yazarlar: Soner Duman | Bulent Oktay | Mustafa Serteser

Yayıncı: Marmara University

Ana Başlıklar: Clinical Pharmacology and Therapeutics

Dergi: Journal of Research in Pharmacy Vol.28 Issue 4, pp.1265-1274

Belge Tipi: Makale

Yayın Tarihi: 6/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001347040

pISSN: 2630-6344

Amino acids

kidney transplantation

amino acid clearance

estimated glomerular filtration rate

creatinine clearance.

Sayı

Yazarlar:

Soner Duman | Bulent Oktay | Mustafa Serteser

Yayıncı:

Marmara University

Ana Başlıklar:

Clinical Pharmacology and Therapeutics

Dergi:

Journal of Research in Pharmacy Vol.28 Issue 4, pp.1265-1274

Belge Tipi:

Makale

Yayın Tarihi:

6/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001347040

pISSN:

2630-6344