Humic Acid Alleviates Lipopolysaccharide-Induced Acute Kidney Injury by Regulating TNF-α Expression

İhsan Karaboğa; Hamza Malik Okuyan; Serdar Doğan; Şeyda Öznur Ayçiçek; Mehmet Doğan; Hafize Kılınçkaya Doğan

Makale detayları

Humic Acid Alleviates Lipopolysaccharide-Induced Acute Kidney Injury by Regulating TNF-α Expression

Yazarlar:

İhsan Karaboğa | Hamza Malik Okuyan | Serdar Doğan | Şeyda Öznur Ayçiçek | Mehmet Doğan | Hafize Kılınçkaya Doğan

Yayıncı:

Gumushane University

Ana Başlıklar:

Acil Tıp

Dergi:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 3, pp.938-946

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001358876

pISSN:

2146-9954

Anahtar kelimeler:

Özet:

 Sepsis-induced acute kidney injury AKI is a clinical condition with a high mortality rate despite advances in health. This study aimed to investigate the potential protective effect of HA in the LPS-induced rat AKI model. A total of 24 adult male Wistar albino rats were used in the study. The rats were randomly divided into three groups: 1 Control group, 2 LPS group and 3 HALPS group. Lipopolysaccharide LPS was administered at a dose of 5 mgkg to the LPS group, while humic acid HA was administered at a dose of 50 mgkg for three days before LPS injection to the HALPS group. Histopathological changes in the renal tissues were evaluated by hematoxylin-eosin HE staining method. In addition, tumor necrosis factor-alpha TNF-α expression levels were examined by immunohistochemical analyses. Malondialdehyde MDA, total antioxidant capacity TAS, total oxidant capacity TOS, superoxide dismutase SOD, glutathione peroxidase GPx as oxidative stress markers were analyzed. Parameters such as blood urea nitrogen BUN, creatinine CREA, lactate dehydrogenase LDH, total protein TP, albumin, uric acid were analyzed in serum samples. Our findings show that HA treatment applied in rat sepsis model reduces renal tubular damage and decreases TNF-α expression. In conclusion, HA may improve renal functions by regulating inflammation in LPS-induced AKI model.

 Sepsis-induced acute kidney injury AKI is a clinical condition with a high mortality rate despite advances in health. This study aimed to investigate the potential protective effect of HA in the LPS-induced rat AKI model. A total of 24 adult male Wistar albino rats were used in the study. The rats were randomly divided into three groups: 1 Control group, 2 LPS group and 3 HALPS group. Lipopolysaccharide LPS was administered at a dose of 5 mgkg to the LPS group, while humic acid HA was administered at a dose of 50 mgkg for three days before LPS injection to the HALPS group. Histopathological changes in the renal tissues were evaluated by hematoxylin-eosin HE staining method. In addition, tumor necrosis factor-alpha TNF-α expression levels were examined by immunohistochemical analyses. Malondialdehyde MDA, total antioxidant capacity TAS, total oxidant capacity TOS, superoxide dismutase SOD, glutathione peroxidase GPx as oxidative stress markers were analyzed. Parameters such as blood urea nitrogen BUN, creatinine CREA, lactate dehydrogenase LDH, total protein TP, albumin, uric acid were analyzed in serum samples. Our findings show that HA treatment applied in rat sepsis model reduces renal tubular damage and decreases TNF-α expression. In conclusion, HA may improve renal functions by regulating inflammation in LPS-induced AKI model.

 Sepsis-induced acute kidney injury AKI is a clinical condition with a high mortality rate despite advances in health. This study aimed to investigate the potential protective effect of HA in the LPS-induced rat AKI model. A total of 24 adult male Wistar albino rats were used in the study. The rats were randomly divided into three groups: 1 Control group, 2 LPS group and 3 HALPS group. Lipopolysaccharide LPS was administered at a dose of 5 mgkg to the LPS group, while humic acid HA was administered at a dose of 50 mgkg for three days before LPS injection to the HALPS group. Histopathological changes in the renal tissues were evaluated by hematoxylin-eosin HE staining method. In addition, tumor necrosis factor-alpha TNF-α expression levels were examined by immunohistochemical analyses. Malondialdehyde MDA, total antioxidant capacity TAS, total oxidant capacity TOS, superoxide dismutase SOD, glutathione peroxidase GPx as oxidative stress markers were analyzed. Parameters such as blood urea nitrogen BUN, creatinine CREA, lactate dehydrogenase LDH, total protein TP, albumin, uric acid were analyzed in serum samples. Our findings show that HA treatment applied in rat sepsis model reduces renal tubular damage and decreases TNF-α expression. In conclusion, HA may improve renal functions by regulating inflammation in LPS-induced AKI model.

 Sepsis-uyarımlı akut böbrek hasarı ABH sağlık alanındaki gelişmelere rağmen yüksek mortalite oranına sahip bir klinik tablodur. Bu çalışmada HAnın LPS ile indüklenen sıçan ABH modelinde potansiyel koruyucu etkisinin incelenmesi amaçlanmıştır. Çalışmada toplam 24 adet erişkin erkek Wistar albino sıçan kullanıldı. Sıçanlar rastgele olarak üç gruba ayrıldı: 1 Kontrol grubu, 2 LPS grubu ve 3 HALPS grubu. LPS grubuna 5 mgkg dozunda Lipopolisakkarit LPS uygulanırken, HALPS grubuna LPS enjeksiyonundan önceki üç gün boyunca 50 mgkg dozunda Humik asit HA tedavisi uygulandı. Böbrek dokularındaki histopatolojik değişiklikler Hematoksilen-Eozin HE boyama yöntemi ile değerlendirildi. Ayrıca, tümör nekroz faktörü-alfa TNF-α ekspresyon düzeyleri immünohistokimyasal analizlerle incelendi. Malondialdehit MDA total antioksidan kapasite TAS, total oksidan kapasite TOS, süperoksit dismutaz SOD, Glutatyon peroksidaz GPx gibi oksidatif stres belirteçleri analiz edildi. Kan üre azotu BUN, kreatinin CREA, laktat dehidrogenaz LDH, total protein TP, albumin, ürik asit gibi parametreler serum numunelerinde analiz edildi. Bulgularımız, sıçan sepsis modelinde uygulanan HA tedavisinin renal tübüler hasarı azalttığını ve TNF-α ekspresyonunu düşürdüğünü göstermektedir. Sonuç olarak, HA, LPS-uyarımlı ABH modelinde inflamasyonu düzenleyerek renal fonksiyonları iyileştirebilir.

Makale detayları

Humic Acid Alleviates Lipopolysaccharide-Induced Acute Kidney Injury by Regulating TNF-α Expression

Yazarlar:

İhsan Karaboğa | Hamza Malik Okuyan | Serdar Doğan | Şeyda Öznur Ayçiçek | Mehmet Doğan | Hafize Kılınçkaya Doğan

Yayıncı:

Gumushane University

Ana Başlıklar:

Acil Tıp

Dergi:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 3, pp.938-946

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001358876

pISSN:

2146-9954

Lipopolysaccharide

acute kidney injury

humic acidlipopolisakkarit

akut böbrek hasarı

humik asit

Sayı

Makale detayları

Humic Acid Alleviates Lipopolysaccharide-Induced Acute Kidney Injury by Regulating TNF-α Expression

Yazarlar: İhsan Karaboğa | Hamza Malik Okuyan | Serdar Doğan | Şeyda Öznur Ayçiçek | Mehmet Doğan | Hafize Kılınçkaya Doğan

Yayıncı: Gumushane University

Ana Başlıklar: Acil Tıp

Dergi: Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 3, pp.938-946

Belge Tipi: Makale

Yayın Tarihi: 9/2025

Belge Dili: Türkçe

Document ID (Org No.): 2001358876

pISSN: 2146-9954

Lipopolysaccharide

acute kidney injury

humic acidlipopolisakkarit

akut böbrek hasarı

humik asit

Sayı

Yazarlar:

İhsan Karaboğa | Hamza Malik Okuyan | Serdar Doğan | Şeyda Öznur Ayçiçek | Mehmet Doğan | Hafize Kılınçkaya Doğan

Yayıncı:

Gumushane University

Ana Başlıklar:

Acil Tıp

Dergi:

Gümüşhane Üniversitesi Sağlık Bilimleri Dergisi Vol.14 Issue 3, pp.938-946

Belge Tipi:

Makale

Yayın Tarihi:

9/2025

Belge Dili:

Türkçe

Document ID (Org No.):

2001358876

pISSN:

2146-9954