In vitro investigations of biological activities of Thymus willdenowii and Thymus atlanticus polyphenol-rich extracts

Elbouny Hamza

Makale detayları

In vitro investigations of biological activities of Thymus willdenowii and Thymus atlanticus polyphenol-rich extracts

Yazarlar:

Elbouny Hamza

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 1, pp.48-58

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283906

pISSN:

2148-6905

Anahtar kelimeler:

Özet:

 Thyme species produce a wide variety of phenolic compounds including tannins, phenolic acids, and flavonoids. Thymus atlanticus T. atlanticus and Thymus willdenowii T. willdenowii are important thyme species in the southeast of Morocco, with numerous biological properties. The polyphenolic extracts of these two thyme species were obtained using ethanol through Soxhlet apparatus. Antioxidant DPPH, FRAP, and TAC methods, antihemolytic 2,2-Azobis2-amidinopropane dihydrochloride AAPH induced hemolysis test, hypolipidemic 3-hydroxy-3-methylglutaryl-coenzyme A HMG-CoA reductase activity inhibition test, and anti-inflammatory protein denaturation inhibition effects of extracts were carried out using in vitro methods. The results showed that the polyphenolic extracts of these two species revealed important amounts of phenolic compounds. The contents of flavonoids were significant in the two species, while the contents of tannins and anthocyanin were very low. T. atlanticus showed an important antioxidant activity and a considerable antihemolytic effect in AAPH-induced hemolysis test IC50  0.29 mgmL, while T. willdenowii showed an important anti-inflammatory activity in heat-induced protein denaturation test IC50  1.61 mgmL. Moreover, both extracts at a dose of 20 µgmL showed an important in vitro hypolipidemic activity by inhibiting HMG-CoA reductase activity T. willdenowii: 51.16 ; T. atlanticus: 62.83 . In conclusion, T. willdenowii and T. atlanticus extracts have considerable antioxidant, antihemolytic, hypolipidemic, and anti-inflammatory effects. The richness of these species in polyphenols gives them a large specter of biological properties, making them a valuable source of natural bioactive compounds that could prevent or treat various diseases.

 Thyme species produce a wide variety of phenolic compounds including tannins, phenolic acids, and flavonoids. Thymus atlanticus T. atlanticus and Thymus willdenowii T. willdenowii are important thyme species in the southeast of Morocco, with numerous biological properties. The polyphenolic extracts of these two thyme species were obtained using ethanol through Soxhlet apparatus. Antioxidant DPPH, FRAP, and TAC methods, antihemolytic 2,2-Azobis2-amidinopropane dihydrochloride AAPH induced hemolysis test, hypolipidemic 3-hydroxy-3-methylglutaryl-coenzyme A HMG-CoA reductase activity inhibition test, and anti-inflammatory protein denaturation inhibition effects of extracts were carried out using in vitro methods. The results showed that the polyphenolic extracts of these two species revealed important amounts of phenolic compounds. The contents of flavonoids were significant in the two species, while the contents of tannins and anthocyanin were very low. T. atlanticus showed an important antioxidant activity and a considerable antihemolytic effect in AAPH-induced hemolysis test IC50  0.29 mgmL, while T. willdenowii showed an important anti-inflammatory activity in heat-induced protein denaturation test IC50  1.61 mgmL. Moreover, both extracts at a dose of 20 µgmL showed an important in vitro hypolipidemic activity by inhibiting HMG-CoA reductase activity T. willdenowii: 51.16 ; T. atlanticus: 62.83 . In conclusion, T. willdenowii and T. atlanticus extracts have considerable antioxidant, antihemolytic, hypolipidemic, and anti-inflammatory effects. The richness of these species in polyphenols gives them a large specter of biological properties, making them a valuable source of natural bioactive compounds that could prevent or treat various diseases.

 Thyme species produce a wide variety of phenolic compounds including tannins, phenolic acids, and flavonoids. Thymus atlanticus T. atlanticus and Thymus willdenowii T. willdenowii are important thyme species in the southeast of Morocco, with numerous biological properties. The polyphenolic extracts of these two thyme species were obtained using ethanol through Soxhlet apparatus. Antioxidant DPPH, FRAP, and TAC methods, antihemolytic 2,2-Azobis2-amidinopropane dihydrochloride AAPH induced hemolysis test, hypolipidemic 3-hydroxy-3-methylglutaryl-coenzyme A HMG-CoA reductase activity inhibition test, and anti-inflammatory protein denaturation inhibition effects of extracts were carried out using in vitro methods. The results showed that the polyphenolic extracts of these two species revealed important amounts of phenolic compounds. The contents of flavonoids were significant in the two species, while the contents of tannins and anthocyanin were very low. T. atlanticus showed an important antioxidant activity and a considerable antihemolytic effect in AAPH-induced hemolysis test IC50  0.29 mgmL, while T. willdenowii showed an important anti-inflammatory activity in heat-induced protein denaturation test IC50  1.61 mgmL. Moreover, both extracts at a dose of 20 µgmL showed an important in vitro hypolipidemic activity by inhibiting HMG-CoA reductase activity T. willdenowii: 51.16 ; T. atlanticus: 62.83 . In conclusion, T. willdenowii and T. atlanticus extracts have considerable antioxidant, antihemolytic, hypolipidemic, and anti-inflammatory effects. The richness of these species in polyphenols gives them a large specter of biological properties, making them a valuable source of natural bioactive compounds that could prevent or treat various diseases.

 Thyme species produce a wide variety of phenolic compounds including tannins, phenolic acids, and flavonoids. Thymus atlanticus T. atlanticus and Thymus willdenowii T. willdenowii are important thyme species in the southeast of Morocco, with numerous biological properties. The polyphenolic extracts of these two thyme species were obtained using ethanol through Soxhlet apparatus. Antioxidant DPPH, FRAP, and TAC methods, antihemolytic 2,2-Azobis2-amidinopropane dihydrochloride AAPH induced hemolysis test, hypolipidemic 3-hydroxy-3-methylglutaryl-coenzyme A HMG-CoA reductase activity inhibition test, and anti-inflammatory protein denaturation inhibition effects of extracts were carried out using in vitro methods. The results showed that the polyphenolic extracts of these two species revealed important amounts of phenolic compounds. The contents of flavonoids were significant in the two species, while the contents of tannins and anthocyanin were very low. T. atlanticus showed an important antioxidant activity and a considerable antihemolytic effect in AAPH-induced hemolysis test IC50  0.29 mgmL, while T. willdenowii showed an important anti-inflammatory activity in heat-induced protein denaturation test IC50  1.61 mgmL. Moreover, both extracts at a dose of 20 µgmL showed an important in vitro hypolipidemic activity by inhibiting HMG-CoA reductase activity T. willdenowii: 51.16 ; T. atlanticus: 62.83 . In conclusion, T. willdenowii and T. atlanticus extracts have considerable antioxidant, antihemolytic, hypolipidemic, and anti-inflammatory effects. The richness of these species in polyphenols gives them a large specter of biological properties, making them a valuable source of natural bioactive compounds that could prevent or treat various diseases.

Makale detayları

In vitro investigations of biological activities of Thymus willdenowii and Thymus atlanticus polyphenol-rich extracts

Yazarlar:

Elbouny Hamza

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 1, pp.48-58

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283906

pISSN:

2148-6905

Thymus atlanticus

Thymus willdenowii

polyphenols

Biological properties

Thymus atlanticus

Thymus willdenowii

polyphenols

biological properties

Sayı

Makale detayları

Yazarlar: Elbouny Hamza

Yayıncı: İzzet KARA

Ana Başlıklar: Pharmacology and Pharmaceutical Sciences

Dergi: International Journal of Secondary Metabolite Vol.10 Issue 1, pp.48-58

Belge Tipi: Makale

Yayın Tarihi: 3/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001283906

pISSN: 2148-6905

Sayı

Yazarlar:

Elbouny Hamza

Yayıncı:

İzzet KARA

Ana Başlıklar:

Pharmacology and Pharmaceutical Sciences

Dergi:

International Journal of Secondary Metabolite Vol.10 Issue 1, pp.48-58

Belge Tipi:

Makale

Yayın Tarihi:

3/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283906

pISSN:

2148-6905