Oxidative status of colitis-associated cancer model induced by azoxymethane /dextran sulfate sodium and the effects of COX-2 inhibitor in mice

Görkem KISMALI; Aykut Göktürk ÜNER; Öğünç MERAL; Merve ALPAY; Berrin SALMANOĞLU; Dilek ÜLKER ÇAKIR; Funda KOSOVA; Tevhide SEL

doi:https://doi.org/10.33988/auvfd.521040

Makale detayları

Oxidative status of colitis-associated cancer model induced by azoxymethane /dextran sulfate sodium and the effects of COX-2 inhibitor in mice

Yazarlar:

Görkem KISMALI | Aykut Göktürk ÜNER | Öğünç MERAL | Merve ALPAY | Berrin SALMANOĞLU | Dilek ÜLKER ÇAKIR | Funda KOSOVA | Tevhide SEL

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 4, pp.351-356

Belge Tipi:

Makale

Yayın Tarihi:

9/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000849577

DOI:

https://doi.org/10.33988/auvfd.521040

Özet:

 


Natural products and
anti-inflammatory agents including cyclooxygenase-2 (COX-2) inhibitors which is
a type of nonsteroidal anti-inflammatory drugs (NSAIDs) are highly considerable
interest for the prevention of carcinogenesis. The objective of this study is
to evaluate the oxidative status of colitis-associated cancer induced by azoxymethane
(AOM)/dextran sulfate sodium (DSS), and the effects of COX-2 inhibitor in mice.
Totally 40 mice were randomized and divided to four groups. All animals except
control and Cox-2 inhibitor alone group received AOM/DSS to establish
colitis-associated cancer model as reported elsewhere. COX-2 preferential
inhibitor meloxicam was used to minimize side effects such as gastrointestinal
hemorrhage. Meloxicam were used (5mg/kg, intraperitoneal) three times a week
with meloxicam alone and AOM/DSS + meloxicam group. Superoxide dismutase (SOD),
Glutathione peroxidase (GPx), Malondialdehyde (MDA) and Advanced Oxidation
Protein Products (AOPP) which all of them are oxidative stress markers were
measured by spectrophotometrically. The combination treatment of Meloxicam and
AOM/DSS significantly increased (P< 0.05) SOD activities in mice. GPx
activities were found significantly increased (P< 0.05) in Meloxicam and
AOM/DSS combinations or alone. There were no differences between the control
and treatment groups of MDA levels. AOPP levels of Meloxicam and AOM/DSS
combination group were found higher than the other groups. Meloxicam and /or
AOM/DSS treatment not caused lipid peroxidations, but increased the antioxidant
enzymes and Advanced Oxidation Protein Products levels.

.

Makale detayları

Oxidative status of colitis-associated cancer model induced by azoxymethane /dextran sulfate sodium and the effects of COX-2 inhibitor in mice

Yazarlar: Görkem KISMALI | Aykut Göktürk ÜNER | Öğünç MERAL | Merve ALPAY | Berrin SALMANOĞLU | Dilek ÜLKER ÇAKIR | Funda KOSOVA | Tevhide SEL

Ana Başlıklar: Hayvancılık

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 4, pp.351-356

Belge Tipi: Makale

Yayın Tarihi: 9/2019

Belge Dili: İngilizce

Document ID (Org No.): 2000849577

DOI: https://doi.org/10.33988/auvfd.521040

Sayı

Yazarlar:

Görkem KISMALI | Aykut Göktürk ÜNER | Öğünç MERAL | Merve ALPAY | Berrin SALMANOĞLU | Dilek ÜLKER ÇAKIR | Funda KOSOVA | Tevhide SEL

Ana Başlıklar:

Hayvancılık

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi 2019 Vol.66 Issue 4, pp.351-356

Belge Tipi:

Makale

Yayın Tarihi:

9/2019

Belge Dili:

İngilizce

Document ID (Org No.):

2000849577

DOI:

https://doi.org/10.33988/auvfd.521040