Determination of Cell Viability, Apoptotic and Necrotic Activities of Boswellia serrata Extract in Liver Cancer (HepG2) and Breast Cancer (MCF7) Cell Lines

Semih Dalkılıç; Lütfiye Kadıoğlu Dalkılıç; Lütfü Uygur; Ömer Kaya

Makale detayları

Determination of Cell Viability, Apoptotic and Necrotic Activities of Boswellia serrata Extract in Liver Cancer (HepG2) and Breast Cancer (MCF7) Cell Lines

Yazarlar:

Semih Dalkılıç | Lütfiye Kadıoğlu Dalkılıç | Lütfü Uygur | Ömer Kaya

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Genetik (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 3, pp.1061-1073

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001320306

pISSN:

2687-3729

Anahtar kelimeler:

Özet:

 Cancer is a very important disease that has existed for a very long time in the history of humanity and poses a great threat to humanity today. Today, cancer remains a global health problem. The scientific world has introduced many innovative treatment approaches, but due to the complexity of cancer cells and their resistance to treatment, significant progress has been made in the fight against cancer, but a definitive cure has not yet been found. Boswellia serrata has been used in many parts of the world for a variety of ailments from ancient times to the present day, comes from the Burseraceae family and is commonly known as white gum. B. serrata is a resin tree with very small and few leaves, as it is a plant species that grows as a small tree or shrub 4-6 meters high in the desert. In this study, B.serrata resin was obtained commercially in Gaziantep province and obtained by extraction in methanol and hexane solvents. The cytotoxic activity of B. serrata resin extract was determined by MTT Assay 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide. Apoptoticnecrotic activity was determined by double staining Hoechst 33342 and propidium iodide. Human breast cancer MCF7 and human liver cancer HepG2 cell lines were used in these experiments.

 Cancer is a very important disease that has existed for a very long time in the history of humanity and poses a great threat to humanity today. Today, cancer remains a global health problem. The scientific world has introduced many innovative treatment approaches, but due to the complexity of cancer cells and their resistance to treatment, significant progress has been made in the fight against cancer, but a definitive cure has not yet been found. Boswellia serrata has been used in many parts of the world for a variety of ailments from ancient times to the present day, comes from the Burseraceae family and is commonly known as white gum. B. serrata is a resin tree with very small and few leaves, as it is a plant species that grows as a small tree or shrub 4-6 meters high in the desert. In this study, B.serrata resin was obtained commercially in Gaziantep province and obtained by extraction in methanol and hexane solvents. The cytotoxic activity of B. serrata resin extract was determined by MTT Assay 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide. Apoptoticnecrotic activity was determined by double staining Hoechst 33342 and propidium iodide. Human breast cancer MCF7 and human liver cancer HepG2 cell lines were used in these experiments.

 Cancer is a very important disease that has existed for a very long time in the history of humanity and poses a great threat to humanity today. Today, cancer remains a global health problem. The scientific world has introduced many innovative treatment approaches, but due to the complexity of cancer cells and their resistance to treatment, significant progress has been made in the fight against cancer, but a definitive cure has not yet been found. Boswellia serrata has been used in many parts of the world for a variety of ailments from ancient times to the present day, comes from the Burseraceae family and is commonly known as white gum. B. serrata is a resin tree with very small and few leaves, as it is a plant species that grows as a small tree or shrub 4-6 meters high in the desert. In this study, B.serrata resin was obtained commercially in Gaziantep province and obtained by extraction in methanol and hexane solvents. The cytotoxic activity of B. serrata resin extract was determined by MTT Assay 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide. Apoptoticnecrotic activity was determined by double staining Hoechst 33342 and propidium iodide. Human breast cancer MCF7 and human liver cancer HepG2 cell lines were used in these experiments.

 Kanser, insanlık tarihinde çok uzun zamandır var olan ve günümüze kadar gelerek insanlık için büyük bir tehdit oluşturan çok önemli bir hastalıktır. Günümüzde kanser küresel bir sağlık sorunu olmaya devam etmektedir. Bilim dünyası birçok yenilikçi tedavi yaklaşımı ortaya çıkarmıştır ancak, kanser hücrelerinin karmaşıklığı ve tedaviye direnç gösterebilmesi nedeniyle kanserle mücadelede önemli ilerlemeler kaydedilmiştir, ancak kesin bir tedavi henüz bulunamamıştır. Boswellia serrata, antik çağlardan günümüze kadar dünyanın birçok yerinde çeşitli rahatsızlıklar için kullanılmıştır, Burseraceae familyasından gelir ve genellikle beyaz sakız olarak bilinir. B. serrata, çölde 4-6 metre yüksekliğinde küçük bir ağaç veya çalı şeklinde yetişen bir bitki türü olduğu için çok küçük ve az yapraklı bir reçine ağacıdır. Bu çalışmada, B. serrata reçinesi Gaziantep ilinde ticari olarak elde edilmiş ve metanol ve hekzan çözücülerinde ekstraksiyon işlemi ile elde edilmiştir. B. serrata reçine ekstraktının sitotoksik aktivitesi MTT Assay 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide ile belirlenmiştir. Apoptotiknekrotik aktivite ikili boyama Hoechst 33342 ve propidium iyodür ile belirlenmiştir. Bu deneylerde insan meme kanseri MCF7 ve insan karaciğer kanseri HepG2 hücre hatları kullanılmıştır.

Makale detayları

Determination of Cell Viability, Apoptotic and Necrotic Activities of Boswellia serrata Extract in Liver Cancer (HepG2) and Breast Cancer (MCF7) Cell Lines

Yazarlar:

Semih Dalkılıç | Lütfiye Kadıoğlu Dalkılıç | Lütfü Uygur | Ömer Kaya

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Genetik (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 3, pp.1061-1073

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001320306

pISSN:

2687-3729

Boswellia serrata

MCF7

HEPG2

Apoptotic

Necrotic

MTTBoswellia serrata

MCF7

HEPG2

Apoptotik

Nekrotik

MTT

Sayı

Makale detayları

Yazarlar: Semih Dalkılıç | Lütfiye Kadıoğlu Dalkılıç | Lütfü Uygur | Ömer Kaya

Yayıncı: Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar: Genetik (Diğer)

Dergi: Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 3, pp.1061-1073

Belge Tipi: Makale

Yayın Tarihi: 6/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001320306

pISSN: 2687-3729

Sayı

Yazarlar:

Semih Dalkılıç | Lütfiye Kadıoğlu Dalkılıç | Lütfü Uygur | Ömer Kaya

Yayıncı:

Osmaniye Korkut Ata Üniversitesi

Ana Başlıklar:

Genetik (Diğer)

Dergi:

Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.7 Issue 3, pp.1061-1073

Belge Tipi:

Makale

Yayın Tarihi:

6/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001320306

pISSN:

2687-3729