Antibacterial, Phytochemical and Toxicological Activities of Garcinia kola Extracts against Multidrug Resistant Clinical Bacteria

Kolawole Oyetunji; Blessing Nwadike

Makale detayları

Antibacterial, Phytochemical and Toxicological Activities of Garcinia kola Extracts against Multidrug Resistant Clinical Bacteria

Yazarlar:

Kolawole Oyetunji | Blessing Nwadike

Yayıncı:

Duzce University

Ana Başlıklar:

Traditional | Complementary and Integrative Medicine (Other)

Dergi:

International Journal of Traditional and Complementary Medicine Research Vol.5 Issue 1, pp.28-38

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316422

pISSN:

2717-7491

Anahtar kelimeler:

Özet:

 Objective: The rise in multiple antibiotic resistant microorganisms has led to a decline in the efficacy of many antibiotics, prompting the investigation of alternative solutions such as medicinal plants. To tackle this concerning issue, this study was undertaken to evaluate the phytochemical composition, antibacterial activity, and toxicological characteristics of Garcinia kola extracts against multidrug-resistant bacteria commonly found in clinical settings. Material-Method: The cold maceration technique was employed to extract the root and leaf of Garcinia kola using water and methanol. The extracts were then subjected to phytochemical screening. To evaluate the extracts potential to inhibit bacterial growth, five multidrug-resistant isolates were utilized in this study. Agar well assay was used to determine the zones of inhibition. The Minimum Inhibitory Concentration MIC and Minimum Bactericidal Concentration MBC were determined using the broth macro dilution technique. Additionally, Brine shrimp lethality assay was conducted to determine the lethal concentration LC50 of the extracts. The extracts were also combined for synergism. Results: The investigation found that the root extract in methanol had the highest yield at 25.58. All four extracts contained ten out of the fourteen tested phytochemicals. The methanol leaf and root extracts exhibited the highest and lowest inhibition zones of 20mm and 9mm against Staphylococcus aureus, respectively. The MIC values ranged from 250 to 600 mgml, indicating broad-spectrum antibacterial activity. The cytotoxicity test showed a range of 33.03 to 126.3µgmL for the aqueous and methanol extracts. Conclusion: Although Garcinia kola shows potential as a source of antibacterial compounds, caution should be exercised due to its toxic effects.

 Objective: The rise in multiple antibiotic resistant microorganisms has led to a decline in the efficacy of many antibiotics, prompting the investigation of alternative solutions such as medicinal plants. To tackle this concerning issue, this study was undertaken to evaluate the phytochemical composition, antibacterial activity, and toxicological characteristics of Garcinia kola extracts against multidrug-resistant bacteria commonly found in clinical settings. Material-Method: The cold maceration technique was employed to extract the root and leaf of Garcinia kola using water and methanol. The extracts were then subjected to phytochemical screening. To evaluate the extracts potential to inhibit bacterial growth, five multidrug-resistant isolates were utilized in this study. Agar well assay was used to determine the zones of inhibition. The Minimum Inhibitory Concentration MIC and Minimum Bactericidal Concentration MBC were determined using the broth macro dilution technique. Additionally, Brine shrimp lethality assay was conducted to determine the lethal concentration LC50 of the extracts. The extracts were also combined for synergism. Results: The investigation found that the root extract in methanol had the highest yield at 25.58. All four extracts contained ten out of the fourteen tested phytochemicals. The methanol leaf and root extracts exhibited the highest and lowest inhibition zones of 20mm and 9mm against Staphylococcus aureus, respectively. The MIC values ranged from 250 to 600 mgml, indicating broad-spectrum antibacterial activity. The cytotoxicity test showed a range of 33.03 to 126.3µgmL for the aqueous and methanol extracts. Conclusion: Although Garcinia kola shows potential as a source of antibacterial compounds, caution should be exercised due to its toxic effects.

 Objective: The rise in multiple antibiotic resistant microorganisms has led to a decline in the efficacy of many antibiotics, prompting the investigation of alternative solutions such as medicinal plants. To tackle this concerning issue, this study was undertaken to evaluate the phytochemical composition, antibacterial activity, and toxicological characteristics of Garcinia kola extracts against multidrug-resistant bacteria commonly found in clinical settings. Material-Method: The cold maceration technique was employed to extract the root and leaf of Garcinia kola using water and methanol. The extracts were then subjected to phytochemical screening. To evaluate the extracts potential to inhibit bacterial growth, five multidrug-resistant isolates were utilized in this study. Agar well assay was used to determine the zones of inhibition. The Minimum Inhibitory Concentration MIC and Minimum Bactericidal Concentration MBC were determined using the broth macro dilution technique. Additionally, Brine shrimp lethality assay was conducted to determine the lethal concentration LC50 of the extracts. The extracts were also combined for synergism. Results: The investigation found that the root extract in methanol had the highest yield at 25.58. All four extracts contained ten out of the fourteen tested phytochemicals. The methanol leaf and root extracts exhibited the highest and lowest inhibition zones of 20mm and 9mm against Staphylococcus aureus, respectively. The MIC values ranged from 250 to 600 mgml, indicating broad-spectrum antibacterial activity. The cytotoxicity test showed a range of 33.03 to 126.3µgmL for the aqueous and methanol extracts. Conclusion: Although Garcinia kola shows potential as a source of antibacterial compounds, caution should be exercised due to its toxic effects.

 Objective: The rise in multiple antibiotic resistant microorganisms has led to a decline in the efficacy of many antibiotics, prompting the investigation of alternative solutions such as medicinal plants. To tackle this concerning issue, this study was undertaken to evaluate the phytochemical composition, antibacterial activity, and toxicological characteristics of Garcinia kola extracts against multidrug-resistant bacteria commonly found in clinical settings. Material-Method: The cold maceration technique was employed to extract the root and leaf of Garcinia kola using water and methanol. The extracts were then subjected to phytochemical screening. To evaluate the extracts potential to inhibit bacterial growth, five multidrug-resistant isolates were utilized in this study. Agar well assay was used to determine the zones of inhibition. The Minimum Inhibitory Concentration MIC and Minimum Bactericidal Concentration MBC were determined using the broth macro dilution technique. Additionally, Brine shrimp lethality assay was conducted to determine the lethal concentration LC50 of the extracts. The extracts were also combined for synergism. Results: The investigation found that the root extract in methanol had the highest yield at 25.58. All four extracts contained ten out of the fourteen tested phytochemicals. The methanol leaf and root extracts exhibited the highest and lowest inhibition zones of 20mm and 9mm against Staphylococcus aureus, respectively. The MIC values ranged from 250 to 600 mgml, indicating broad-spectrum antibacterial activity. The cytotoxicity test showed a range of 33.03 to 126.3µgmL for the aqueous and methanol extracts. Conclusion: Although Garcinia kola shows potential as a source of antibacterial compounds, caution should be exercised due to its toxic effects.

Makale detayları

Antibacterial, Phytochemical and Toxicological Activities of Garcinia kola Extracts against Multidrug Resistant Clinical Bacteria

Yazarlar:

Kolawole Oyetunji | Blessing Nwadike

Yayıncı:

Duzce University

Ana Başlıklar:

Traditional | Complementary and Integrative Medicine (Other)

Dergi:

International Journal of Traditional and Complementary Medicine Research Vol.5 Issue 1, pp.28-38

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316422

pISSN:

2717-7491

Garcinia kola

Phytochemicals

Minimum Inhibitory Concentration [MIC]

Inhibition zones

Cytotoxicity.

Sayı

Makale detayları

Antibacterial, Phytochemical and Toxicological Activities of Garcinia kola Extracts against Multidrug Resistant Clinical Bacteria

Yazarlar: Kolawole Oyetunji | Blessing Nwadike

Yayıncı: Duzce University

Ana Başlıklar: Traditional | Complementary and Integrative Medicine (Other)

Dergi: International Journal of Traditional and Complementary Medicine Research Vol.5 Issue 1, pp.28-38

Belge Tipi: Makale

Yayın Tarihi: 4/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001316422

pISSN: 2717-7491

Garcinia kola

Phytochemicals

Minimum Inhibitory Concentration [MIC]

Inhibition zones

Cytotoxicity.

Sayı

Yazarlar:

Kolawole Oyetunji | Blessing Nwadike

Yayıncı:

Duzce University

Ana Başlıklar:

Traditional | Complementary and Integrative Medicine (Other)

Dergi:

International Journal of Traditional and Complementary Medicine Research Vol.5 Issue 1, pp.28-38

Belge Tipi:

Makale

Yayın Tarihi:

4/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001316422

pISSN:

2717-7491