Investigation of the effects of electrical stimulation on BDNF and NGF levels in the sciatic nerve injury rat model

Egemen Işık; Filiz Kazak; Ziya Yurtal; Halil Alakuş

Makale detayları

Investigation of the effects of electrical stimulation on BDNF and NGF levels in the sciatic nerve injury rat model

Yazarlar:

Egemen Işık | Filiz Kazak | Ziya Yurtal | Halil Alakuş

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Biochemistry

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 2, pp.175-181

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343144

pISSN:

1300-0861

Anahtar kelimeler:

Özet:

 The current study aimed to investigate the effects of electrical stimulation on brain-derived neurotrophic factor BDNF and nerve growth factor NGF levels in rats with sciatic nerve injury. Twenty-eight rats were divided into four groups of sham S, n7, electrical stimulation ES, n7, sciatic nerve injury SNI, n7 and sciatic nerve injuryelectrical stimulation SNIES, n7. An experimental nerve damage model was produced by applying a closing force to compress the sciatic nerve. Electrical stimulation was applied for twenty minutes at 200 μs, 2mA, and 20 Hz for fifteen days. Enzyme-linked immunosorbent assay analysis was used to evaluate the levels of NGF and BDNF. It was shown that the SNI group had higher brain BDNF levels than the other groups, while the S group had lower brain BDNF levels than the other groups P0.001. The ES and SNI groups had higher serum BDNF levels than the S and SNIES groups P0.01, while the SNI group had higher brain NGF levels than the S and SNIES groups P0.05. In comparison to the S and ES groups, the serum NGF levels in the SNI and SNIES groups were shown to be lower P0.01. Following the sciatic nerve damage, it was measured that the administration of electrical stimulation resulted in an increase in brain BDNF and a decrease in serum NGF. According to this research, electrical stimulation may have an impact on the release of NGF and BDNF after sciatic nerve damage.

 The current study aimed to investigate the effects of electrical stimulation on brain-derived neurotrophic factor BDNF and nerve growth factor NGF levels in rats with sciatic nerve injury. Twenty-eight rats were divided into four groups of sham S, n7, electrical stimulation ES, n7, sciatic nerve injury SNI, n7 and sciatic nerve injuryelectrical stimulation SNIES, n7. An experimental nerve damage model was produced by applying a closing force to compress the sciatic nerve. Electrical stimulation was applied for twenty minutes at 200 μs, 2mA, and 20 Hz for fifteen days. Enzyme-linked immunosorbent assay analysis was used to evaluate the levels of NGF and BDNF. It was shown that the SNI group had higher brain BDNF levels than the other groups, while the S group had lower brain BDNF levels than the other groups P0.001. The ES and SNI groups had higher serum BDNF levels than the S and SNIES groups P0.01, while the SNI group had higher brain NGF levels than the S and SNIES groups P0.05. In comparison to the S and ES groups, the serum NGF levels in the SNI and SNIES groups were shown to be lower P0.01. Following the sciatic nerve damage, it was measured that the administration of electrical stimulation resulted in an increase in brain BDNF and a decrease in serum NGF. According to this research, electrical stimulation may have an impact on the release of NGF and BDNF after sciatic nerve damage.

 The current study aimed to investigate the effects of electrical stimulation on brain-derived neurotrophic factor BDNF and nerve growth factor NGF levels in rats with sciatic nerve injury. Twenty-eight rats were divided into four groups of sham S, n7, electrical stimulation ES, n7, sciatic nerve injury SNI, n7 and sciatic nerve injuryelectrical stimulation SNIES, n7. An experimental nerve damage model was produced by applying a closing force to compress the sciatic nerve. Electrical stimulation was applied for twenty minutes at 200 μs, 2mA, and 20 Hz for fifteen days. Enzyme-linked immunosorbent assay analysis was used to evaluate the levels of NGF and BDNF. It was shown that the SNI group had higher brain BDNF levels than the other groups, while the S group had lower brain BDNF levels than the other groups P0.001. The ES and SNI groups had higher serum BDNF levels than the S and SNIES groups P0.01, while the SNI group had higher brain NGF levels than the S and SNIES groups P0.05. In comparison to the S and ES groups, the serum NGF levels in the SNI and SNIES groups were shown to be lower P0.01. Following the sciatic nerve damage, it was measured that the administration of electrical stimulation resulted in an increase in brain BDNF and a decrease in serum NGF. According to this research, electrical stimulation may have an impact on the release of NGF and BDNF after sciatic nerve damage.

 The current study aimed to investigate the effects of electrical stimulation on brain-derived neurotrophic factor BDNF and nerve growth factor NGF levels in rats with sciatic nerve injury. Twenty-eight rats were divided into four groups of sham S, n7, electrical stimulation ES, n7, sciatic nerve injury SNI, n7 and sciatic nerve injuryelectrical stimulation SNIES, n7. An experimental nerve damage model was produced by applying a closing force to compress the sciatic nerve. Electrical stimulation was applied for twenty minutes at 200 μs, 2mA, and 20 Hz for fifteen days. Enzyme-linked immunosorbent assay analysis was used to evaluate the levels of NGF and BDNF. It was shown that the SNI group had higher brain BDNF levels than the other groups, while the S group had lower brain BDNF levels than the other groups P0.001. The ES and SNI groups had higher serum BDNF levels than the S and SNIES groups P0.01, while the SNI group had higher brain NGF levels than the S and SNIES groups P0.05. In comparison to the S and ES groups, the serum NGF levels in the SNI and SNIES groups were shown to be lower P0.01. Following the sciatic nerve damage, it was measured that the administration of electrical stimulation resulted in an increase in brain BDNF and a decrease in serum NGF. According to this research, electrical stimulation may have an impact on the release of NGF and BDNF after sciatic nerve damage.

Makale detayları

Investigation of the effects of electrical stimulation on BDNF and NGF levels in the sciatic nerve injury rat model

Yazarlar:

Egemen Işık | Filiz Kazak | Ziya Yurtal | Halil Alakuş

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Biochemistry

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 2, pp.175-181

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343144

pISSN:

1300-0861

Brain-derived neurotrophic factor

electrical stimulation

nerve growth factor

sciatic nerve.

Sayı

Makale detayları

Investigation of the effects of electrical stimulation on BDNF and NGF levels in the sciatic nerve injury rat model

Yazarlar: Egemen Işık | Filiz Kazak | Ziya Yurtal | Halil Alakuş

Yayıncı: Ankara University

Ana Başlıklar: Veterinary Biochemistry

Dergi: Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 2, pp.175-181

Belge Tipi: Makale

Yayın Tarihi: 1/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001343144

pISSN: 1300-0861

Brain-derived neurotrophic factor

electrical stimulation

nerve growth factor

sciatic nerve.

Sayı

Yazarlar:

Egemen Işık | Filiz Kazak | Ziya Yurtal | Halil Alakuş

Yayıncı:

Ankara University

Ana Başlıklar:

Veterinary Biochemistry

Dergi:

Ankara Üniversitesi Veteriner Fakültesi Dergisi Vol.72 Issue 2, pp.175-181

Belge Tipi:

Makale

Yayın Tarihi:

1/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001343144

pISSN:

1300-0861