Vertical transmission of porcine circovirus 2b (PCV2b) to mouse fetuses in maternal uterus by artificial insemination with semen from PCV2b-infected male mouse

Deng ZHIBANG; Gong QIANLONG; Wang NAIDONG; Yuan ANWEN; Yu Xing LONG

Makale detayları

Vertical transmission of porcine circovirus 2b (PCV2b) to mouse fetuses in maternal uterus by artificial insemination with semen from PCV2b-infected male mouse

Yazarlar:

Deng ZHIBANG | Gong QIANLONG | Wang NAIDONG | Yuan ANWEN | Yu Xing LONG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 4, pp.475-478

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766408

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Anahtar kelimeler:

Özet:

 

In order to investigate whether porcine circovirus 2b (PCV2b) can be replicated in the spermatids of mice and whether semen from PCV2b-infected male mice can induce reproductive failure in female mice, 10 male Kunming mice were intraperitoneally inoculated with PCV2b, and 10 female Kunming mice were artificial inseminated with semen from the PCV2b-inoculated male mice. Semen from inoculated male mice and newborn mice or dead fetuses from inseminated female mice were tested for PCV2b by polymerase chain reaction, transmission electron microscopy, and immunofluorescent histochemistry. The results showed that PCV2b was capable of being replicated in spermatids in Kunming mice and could be transmitted from semen to fetuses by artificial insemination, causing reproductive failure in pregnant Kunming mice and PCV2b vertical infection to the newborn mice.

 

In order to investigate whether porcine circovirus 2b (PCV2b) can be replicated in the spermatids of mice and whether semen from PCV2b-infected male mice can induce reproductive failure in female mice, 10 male Kunming mice were intraperitoneally inoculated with PCV2b, and 10 female Kunming mice were artificial inseminated with semen from the PCV2b-inoculated male mice. Semen from inoculated male mice and newborn mice or dead fetuses from inseminated female mice were tested for PCV2b by polymerase chain reaction, transmission electron microscopy, and immunofluorescent histochemistry. The results showed that PCV2b was capable of being replicated in spermatids in Kunming mice and could be transmitted from semen to fetuses by artificial insemination, causing reproductive failure in pregnant Kunming mice and PCV2b vertical infection to the newborn mice.

Makale detayları

Vertical transmission of porcine circovirus 2b (PCV2b) to mouse fetuses in maternal uterus by artificial insemination with semen from PCV2b-infected male mouse

Yazarlar:

Deng ZHIBANG | Gong QIANLONG | Wang NAIDONG | Yuan ANWEN | Yu Xing LONG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 4, pp.475-478

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766408

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128

Key words: PCV2b DNA-positive semen

artificial insemination

PCV2b transmission to fetuses

reproductive failure

Kunming mice

Sayı

Makale detayları

Vertical transmission of porcine circovirus 2b (PCV2b) to mouse fetuses in maternal uterus by artificial insemination with semen from PCV2b-infected male mouse

Yazarlar: Deng ZHIBANG | Gong QIANLONG | Wang NAIDONG | Yuan ANWEN | Yu Xing LONG

Yayıncı: TUBITAK

Dergi: Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 4, pp.475-478

Belge Tipi: Makale

Yayın Tarihi: 3/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000766408

Sıklık: İki Aylık

eISSN: 1303-6181

pISSN: 1300-0128

Key words: PCV2b DNA-positive semen

artificial insemination

PCV2b transmission to fetuses

reproductive failure

Kunming mice

Sayı

Yazarlar:

Deng ZHIBANG | Gong QIANLONG | Wang NAIDONG | Yuan ANWEN | Yu Xing LONG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Veterinary and Animal Sciences 2013 Vol.37 Issue 4, pp.475-478

Belge Tipi:

Makale

Yayın Tarihi:

3/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000766408

Sıklık:

İki Aylık

eISSN:

1303-6181

pISSN:

1300-0128