Experimental Investigations on the Antioxidant, Anti-Quorum Sensing, and Antibiofilm Activities of Ethanolic Extracts from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys, and Thymus longicaulis subsp. longicaulis

Ayşegül Akkoyunlu; Gorkem Dulger; Bahar Gedik; Başaran Dülger

Makale detayları

Experimental Investigations on the Antioxidant, Anti-Quorum Sensing, and Antibiofilm Activities of Ethanolic Extracts from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys, and Thymus longicaulis subsp. longicaulis

Yazarlar:

Ayşegül Akkoyunlu | Gorkem Dulger | Bahar Gedik | Başaran Dülger

Yayıncı:

Duzce University

Ana Başlıklar:

Phytotherapy

Dergi:

International Journal of Traditional and Complementary Medicine Research Vol.6 Issue 2, pp.102-110

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357140

pISSN:

2717-7491

Anahtar kelimeler:

Özet:

 Objective: The aim of this study was to evaluate the oxidant and antioxidant capacities, anti-quorum detection QS and antibiofilm activities of ethanol extracts obtained from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys and Thymus longicaulis subsp. longicaulis. Materials and Methods: Antibiofilm activities were evaluated using the microplate method against a biofilm-forming Escherichia coli reporter strain. Anti-QS activities were evaluated qualitatively using the well diffusion method with Chromobacterium violaceum ATCC 12472 as a biosensor. Total antioxidant status TAS, total oxidant status TOS and oxidative stress index OSI were measured using commercial kits. Results: All three plant extracts showed strong biofilm inhibition. T. chamaedrys subsp. chamaedrys showed the highest antibiofilm activity with 94.07 inhibition at 12.5 µgmL. In the anti-QS screening, T. longicaulis subsp. longicaulis extract showed the largest violacein inhibition zone 21.673.51 mm at 75 mgmL. This extract also exhibited the most favorable oxidative profile with the highest TAS and lowest OSI values among the tested samples. Conclusion: Ethanol extracts of the tested medicinal plants, especially T. longicaulis subsp. longicaulis, have significant antibiofilm, anti-QS and antioxidant activities. The findings indicate the potential of the extracts as natural agent sources to control the virulence properties of pathogens.

 Objective: The aim of this study was to evaluate the oxidant and antioxidant capacities, anti-quorum detection QS and antibiofilm activities of ethanol extracts obtained from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys and Thymus longicaulis subsp. longicaulis. Materials and Methods: Antibiofilm activities were evaluated using the microplate method against a biofilm-forming Escherichia coli reporter strain. Anti-QS activities were evaluated qualitatively using the well diffusion method with Chromobacterium violaceum ATCC 12472 as a biosensor. Total antioxidant status TAS, total oxidant status TOS and oxidative stress index OSI were measured using commercial kits. Results: All three plant extracts showed strong biofilm inhibition. T. chamaedrys subsp. chamaedrys showed the highest antibiofilm activity with 94.07 inhibition at 12.5 µgmL. In the anti-QS screening, T. longicaulis subsp. longicaulis extract showed the largest violacein inhibition zone 21.673.51 mm at 75 mgmL. This extract also exhibited the most favorable oxidative profile with the highest TAS and lowest OSI values among the tested samples. Conclusion: Ethanol extracts of the tested medicinal plants, especially T. longicaulis subsp. longicaulis, have significant antibiofilm, anti-QS and antioxidant activities. The findings indicate the potential of the extracts as natural agent sources to control the virulence properties of pathogens.

 Objective: The aim of this study was to evaluate the oxidant and antioxidant capacities, anti-quorum detection QS and antibiofilm activities of ethanol extracts obtained from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys and Thymus longicaulis subsp. longicaulis. Materials and Methods: Antibiofilm activities were evaluated using the microplate method against a biofilm-forming Escherichia coli reporter strain. Anti-QS activities were evaluated qualitatively using the well diffusion method with Chromobacterium violaceum ATCC 12472 as a biosensor. Total antioxidant status TAS, total oxidant status TOS and oxidative stress index OSI were measured using commercial kits. Results: All three plant extracts showed strong biofilm inhibition. T. chamaedrys subsp. chamaedrys showed the highest antibiofilm activity with 94.07 inhibition at 12.5 µgmL. In the anti-QS screening, T. longicaulis subsp. longicaulis extract showed the largest violacein inhibition zone 21.673.51 mm at 75 mgmL. This extract also exhibited the most favorable oxidative profile with the highest TAS and lowest OSI values among the tested samples. Conclusion: Ethanol extracts of the tested medicinal plants, especially T. longicaulis subsp. longicaulis, have significant antibiofilm, anti-QS and antioxidant activities. The findings indicate the potential of the extracts as natural agent sources to control the virulence properties of pathogens.

 Amaç: Bu çalışmanın amacı Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys ve Thymus longicaulis subsp. longicaulisten elde edilen etanol özütlerinin oksidan ve antioksidan kapasitelerini, anti-quorum tespiti QS ve antibiyofilm aktivitelerini değerlendirmektir. Gereçler ve Yöntemler: Antibiyofilm aktiviteleri, biyofilm oluşturan bir Escherichia coli raportör suşuna karşı mikro plaka yöntemi kullanılarak değerlendirildi. Anti-QS aktiviteleri, biyosensör olarak Chromobacterium violaceum ATCC 12472 ile kuyu difüzyon yöntemi kullanılarak nitel olarak değerlendirildi. Toplam antioksidan durumu TAS, toplam oksidan durumu TOS ve oksidatif stres indeksi OSI ticari kitler kullanılarak ölçüldü. Sonuçlar: Üç bitki özütü de güçlü biyofilm inhibisyonu gösterdi. T. chamaedrys subsp. chamaedrys, 12,5 µgmLde 94,07 inhibisyonla en yüksek antibiyofilm aktivitesini gösterdi. Anti-QS taramasında, T. longicaulis subsp. longicaulis özütü, 75 mgmLde en büyük violacein inhibisyon bölgesini 21,673,51 mm gösterdi. Bu özüt ayrıca test edilen örnekler arasında en yüksek TAS ve en düşük OSI değerleriyle en uygun oksidatif profili sergiledi. Sonuç: Test edilen tıbbi bitkilerin, özellikle T. longicaulis subsp. longicaulisin etanol özütleri, önemli antibiyofilm, anti-QS ve antioksidan aktivitelere sahiptir. Bulgular, özütlerin patojenlerin virülans özelliklerini kontrol etmek için doğal ajan kaynakları olarak potansiyelini göstermektedir.

Makale detayları

Experimental Investigations on the Antioxidant, Anti-Quorum Sensing, and Antibiofilm Activities of Ethanolic Extracts from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys, and Thymus longicaulis subsp. longicaulis

Yazarlar:

Ayşegül Akkoyunlu | Gorkem Dulger | Bahar Gedik | Başaran Dülger

Yayıncı:

Duzce University

Ana Başlıklar:

Phytotherapy

Dergi:

International Journal of Traditional and Complementary Medicine Research Vol.6 Issue 2, pp.102-110

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357140

pISSN:

2717-7491

biofilm

Chromobacterium violaceum ATCC 12472

oxidative stres indexBiyofilm

Chromobacterium violaceum ATCC 12472

Oksidatif stres indeksi

Sayı

Makale detayları

Experimental Investigations on the Antioxidant, Anti-Quorum Sensing, and Antibiofilm Activities of Ethanolic Extracts from Prunella vulgaris, Teucrium chamaedrys subsp. chamaedrys, and Thymus longicaulis subsp. longicaulis

Yazarlar: Ayşegül Akkoyunlu | Gorkem Dulger | Bahar Gedik | Başaran Dülger

Yayıncı: Duzce University

Ana Başlıklar: Phytotherapy

Dergi: International Journal of Traditional and Complementary Medicine Research Vol.6 Issue 2, pp.102-110

Belge Tipi: Makale

Yayın Tarihi: 8/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001357140

pISSN: 2717-7491

biofilm

Chromobacterium violaceum ATCC 12472

oxidative stres indexBiyofilm

Chromobacterium violaceum ATCC 12472

Oksidatif stres indeksi

Sayı

Yazarlar:

Ayşegül Akkoyunlu | Gorkem Dulger | Bahar Gedik | Başaran Dülger

Yayıncı:

Duzce University

Ana Başlıklar:

Phytotherapy

Dergi:

International Journal of Traditional and Complementary Medicine Research Vol.6 Issue 2, pp.102-110

Belge Tipi:

Makale

Yayın Tarihi:

8/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001357140

pISSN:

2717-7491