Molecular characterization of tomato spotted wilt virus (TSWV) and cucumber mosaic virus (CMV) affecting tomato and pepper crops in Izmir Province

Nihan Güneş; İsmail Can Paylan; Mustafa Gümüş

Makale detayları

Molecular characterization of tomato spotted wilt virus (TSWV) and cucumber mosaic virus (CMV) affecting tomato and pepper crops in Izmir Province

Yazarlar:

Nihan Güneş | İsmail Can Paylan | Mustafa Gümüş

Yayıncı:

Ege University

Ana Başlıklar:

Ziraat | Veterinerlik ve Gıda Bilimleri

Dergi:

Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.60 Issue 1, pp.67-79

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284150

pISSN:

1018-8851

Anahtar kelimeler:

Özet:

 Objective: The objective of this study was to investigate tomato spotted wilt virus TSWV and cucumber mosaic virus CMV infections in tomato and pepper plants showing virus-induced symptoms in vegetable growing districts of İzmir, Turkey. Material and Methods: Surveys were carried out in tomato and pepper plantations in 2019 and 2021, and the incidences of these viruses in the collected leaf samples were determined by RT-PCR. Nucleotide identities and phylogenetic relationships of the TSWV and CMV isolates with other isolates retrieved from the GenBank database were determined. Results: The results of this study showed that tomato plants were infected at the same rate 21.50 with TSWV and CMV. Out of the tested pepper samples, 64.15 were infected with TSWV and 25.47 with CMV. The results showed that, the identity rate of nucleoprotein region of TSWV isolates from tomato was 99-96 at nucleotide level while the isolates from pepper showed 100-95 identity. On the other hand, the capsid protein gene region of the tomato isolate of CMV had nucleotide identity rate of 98-95 with other isolates in GenBank, while that of its pepper isolates had 100-98 identity. Also, CMV isolates of this study showed close phylogenetic relationship with the CMV isolates of subgroup IB. Conclusion: This study revealed the prevalence of TSWV and CMV in symptomatic tomato and pepper samples in İzmir province and some molecular properties of them.

 Objective: The objective of this study was to investigate tomato spotted wilt virus TSWV and cucumber mosaic virus CMV infections in tomato and pepper plants showing virus-induced symptoms in vegetable growing districts of İzmir, Turkey. Material and Methods: Surveys were carried out in tomato and pepper plantations in 2019 and 2021, and the incidences of these viruses in the collected leaf samples were determined by RT-PCR. Nucleotide identities and phylogenetic relationships of the TSWV and CMV isolates with other isolates retrieved from the GenBank database were determined. Results: The results of this study showed that tomato plants were infected at the same rate 21.50 with TSWV and CMV. Out of the tested pepper samples, 64.15 were infected with TSWV and 25.47 with CMV. The results showed that, the identity rate of nucleoprotein region of TSWV isolates from tomato was 99-96 at nucleotide level while the isolates from pepper showed 100-95 identity. On the other hand, the capsid protein gene region of the tomato isolate of CMV had nucleotide identity rate of 98-95 with other isolates in GenBank, while that of its pepper isolates had 100-98 identity. Also, CMV isolates of this study showed close phylogenetic relationship with the CMV isolates of subgroup IB. Conclusion: This study revealed the prevalence of TSWV and CMV in symptomatic tomato and pepper samples in İzmir province and some molecular properties of them.

 Objective: The objective of this study was to investigate tomato spotted wilt virus TSWV and cucumber mosaic virus CMV infections in tomato and pepper plants showing virus-induced symptoms in vegetable growing districts of İzmir, Turkey. Material and Methods: Surveys were carried out in tomato and pepper plantations in 2019 and 2021, and the incidences of these viruses in the collected leaf samples were determined by RT-PCR. Nucleotide identities and phylogenetic relationships of the TSWV and CMV isolates with other isolates retrieved from the GenBank database were determined. Results: The results of this study showed that tomato plants were infected at the same rate 21.50 with TSWV and CMV. Out of the tested pepper samples, 64.15 were infected with TSWV and 25.47 with CMV. The results showed that, the identity rate of nucleoprotein region of TSWV isolates from tomato was 99-96 at nucleotide level while the isolates from pepper showed 100-95 identity. On the other hand, the capsid protein gene region of the tomato isolate of CMV had nucleotide identity rate of 98-95 with other isolates in GenBank, while that of its pepper isolates had 100-98 identity. Also, CMV isolates of this study showed close phylogenetic relationship with the CMV isolates of subgroup IB. Conclusion: This study revealed the prevalence of TSWV and CMV in symptomatic tomato and pepper samples in İzmir province and some molecular properties of them.

 Amaç: Bu çalışmanın amacı, İzmirin sebze yetiştirilen önemli ilçelerinde virüs kaynaklı semptomlar gösteren domates ve biber bitkilerinde tomato spotted wilt virus TSWV ve cucumber mosaic virus CMV enfeksiyonlarının araştırılmasıdır. Materyal ve Yöntem: Domates ve biber üretim alanlarında 2019 ve 2021 yıllarında sürveyler yapılmış ve toplanan yaprak örneklerinde bu virüslerin enfeksiyon oranları RT-PCR ile belirlenmiştir. TSWV ve CMV izolatlarının GenBank veri tabanından elde edilen diğer izolatlarla benzerlik oranları ve filogenetik ilişkileri belirlenmiştir. Araştırma Bulguları: Çalışma sonucunda domates bitkilerinin aynı oranda 21,50 TSWV ve CMV ile enfekteli olduğu belirlenmiştir. Test edilen biber örneklerinin 64,15inin TSWV ve 25,47sinin CMV ile enfekteli olduğu tespit edilmiştir. Sonuçlar, TSWV domates izolatlarının nükleokapsid protein gen bölgesinin nükleotid benzerlik oranının 99-96 olduğunu, TSWV biber izolatının ise 100-95 benzerlik gösterdiğini ortaya koymuştur. CMV-domates izolatının kapsid protein gen bölgesi GenBanktaki diğer izolatlarla 98-95 arasında, CMV-biber izolatınınki ise 100-98 arasında nükleotid benzerliğine sahip olmuştur. Ayrıca, CMV izolatları, alt grup IB CMV izolatları ile yakın filogenetik ilişki göstermiştir. Sonuç: Bu çalışma, İzmir ilinde simptomlu domates ve biber örneklerinde TSWV ve CMVnin bulaşıklık oranını ve moleküler özelliklerini ortaya koymuştur.

Makale detayları

Molecular characterization of tomato spotted wilt virus (TSWV) and cucumber mosaic virus (CMV) affecting tomato and pepper crops in Izmir Province

Yazarlar:

Nihan Güneş | İsmail Can Paylan | Mustafa Gümüş

Yayıncı:

Ege University

Ana Başlıklar:

Ziraat | Veterinerlik ve Gıda Bilimleri

Dergi:

Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.60 Issue 1, pp.67-79

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284150

pISSN:

1018-8851

Pepper

solanaceous crops

tomato

virus

RT-PCR

Biber

RT-PCR

patlıcangil

domates

virüs

Sayı

Makale detayları

Yazarlar: Nihan Güneş | İsmail Can Paylan | Mustafa Gümüş

Yayıncı: Ege University

Ana Başlıklar: Ziraat | Veterinerlik ve Gıda Bilimleri

Dergi: Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.60 Issue 1, pp.67-79

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001284150

pISSN: 1018-8851

Sayı

Yazarlar:

Nihan Güneş | İsmail Can Paylan | Mustafa Gümüş

Yayıncı:

Ege University

Ana Başlıklar:

Ziraat | Veterinerlik ve Gıda Bilimleri

Dergi:

Ege Üniversitesi Ziraat Fakültesi Dergisi Vol.60 Issue 1, pp.67-79

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001284150

pISSN:

1018-8851