Protease Activity of a 90-kDa Protein Isolated from Scallop Shells

Manabu FUKUDA; Yasushi HASEGAWA

Makale detayları

Protease Activity of a 90-kDa Protein Isolated from Scallop Shells

Yazarlar:

Manabu FUKUDA | Yasushi HASEGAWA

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2014 Vol.14 Issue 1

Belge Tipi:

Makale

Yayın Tarihi:

7/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000838157

Sıklık:

Üç Aylık

pISSN:

1303-2712

Anahtar kelimeler:

-

Özet:

 

We have previously reported the free radical scavenging activity of a protein with a molecular weight of 90 kDa (90kDa protein) isolated from the scallop shell. In this study, we found that the 90-kDa protein also shows protease activity. The protein was most active at an alkali pH and at 60°C, and its activity was inhibited by serine protease inhibitors, phenylmethylsulfonyl fluoride and diisopropyl fluorophosphate. Its activity was maintained at approximately 90% of the initial activity, even in the presence of denaturants such as 1% sodium dodecyl sulfate (SDS) and 6 M urea. Substrate specificity analysis performed using synthetic peptides showed that the 90-kDa protein cleaves preferentially at Lys-X and Arg-X bonds. A portion of Phe-X bond was also cleaved by the 90-kDa protein. When casein was treated with the 90-kDa protein, it was digested at the Arg-X, Lys-X, and Phe-X bonds. The 90-kDa protein may be useful for proteome analysis because it retains its activity even in the presence of 1% SDS. To the best of our knowledge, this is the first report of a protease found in scallop shell.

Makale detayları

Protease Activity of a 90-kDa Protein Isolated from Scallop Shells

Yazarlar: Manabu FUKUDA | Yasushi HASEGAWA

Yayıncı: Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi: Turkish Journal of Fisheries and Aquatic Sciences 2014 Vol.14 Issue 1

Belge Tipi: Makale

Yayın Tarihi: 7/2014

Belge Dili: Türkçe

Document ID (Org No.): 2000838157

Sıklık: Üç Aylık

pISSN: 1303-2712

-

Sayı

Yazarlar:

Manabu FUKUDA | Yasushi HASEGAWA

Yayıncı:

Trabzon Su Ürünleri Merkez Araştırma Enstitüsü

Dergi:

Turkish Journal of Fisheries and Aquatic Sciences 2014 Vol.14 Issue 1

Belge Tipi:

Makale

Yayın Tarihi:

7/2014

Belge Dili:

Türkçe

Document ID (Org No.):

2000838157

Sıklık:

Üç Aylık

pISSN:

1303-2712