Investigation of 5-Fluorouracil-Induced Cell Death in HCT116 and HT29 Colon Cancer Cells by Fourier Transform Infrared Spectroscopy

Dilşad Özerkan; Naci Tüzemen

Makale detayları

Investigation of 5-Fluorouracil-Induced Cell Death in HCT116 and HT29 Colon Cancer Cells by Fourier Transform Infrared Spectroscopy

Yazarlar:

Dilşad Özerkan | Naci Tüzemen

Yayıncı:

Duzce University

Ana Başlıklar:

Mühendislik

Dergi:

Düzce Üniversitesi Bilim ve Teknoloji Dergisi Vol.11 Issue 1, pp.524-535

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001282903

pISSN:

2148-2446

Anahtar kelimeler:

Özet:

 Colon cancer is a common and highly lethal type of cancer all over the world. As with all cancers, monitoring the course of colon cancer is critical. 5-Fluorouracil 5-FU is an anticancer drug frequently used in colon cancers and causes some biochemical and molecular differences in cells during cell death. In this study, after incubation of HCT116 and HT29 colon cancer cells with 5-FU chemotherapy drug, Fourier transform infrared FTIR spectroscopy was used to detect the biochemical change caused by 5FU on cells. The concentration IC50 of 5-FU at which percent inhibition equals 50 was determined using the MTT method. FTIR measurements were taken after the IC50 value was determined. Accordingly, the IC50 value of 5-FU was found to be 12.69 µgml for HCT116 cells and 10.10 µgml for HT29 cells, respectively. FTIR spectroscopy showed changes in IR biomarkers such as the increase in lipid content, which is said to be indicators of cell death caused by 5-FU, and the nucleic acid ratio, the α-helix and β-sheet secondary structures of total cellular proteins, and the reduction of amino acid residues, and changes in collagen conformation. As a result, examining cancer cell death with FTIR, which is both a cheap and fast method, can be used as an alternative method.

 Colon cancer is a common and highly lethal type of cancer all over the world. As with all cancers, monitoring the course of colon cancer is critical. 5-Fluorouracil 5-FU is an anticancer drug frequently used in colon cancers and causes some biochemical and molecular differences in cells during cell death. In this study, after incubation of HCT116 and HT29 colon cancer cells with 5-FU chemotherapy drug, Fourier transform infrared FTIR spectroscopy was used to detect the biochemical change caused by 5FU on cells. The concentration IC50 of 5-FU at which percent inhibition equals 50 was determined using the MTT method. FTIR measurements were taken after the IC50 value was determined. Accordingly, the IC50 value of 5-FU was found to be 12.69 µgml for HCT116 cells and 10.10 µgml for HT29 cells, respectively. FTIR spectroscopy showed changes in IR biomarkers such as the increase in lipid content, which is said to be indicators of cell death caused by 5-FU, and the nucleic acid ratio, the α-helix and β-sheet secondary structures of total cellular proteins, and the reduction of amino acid residues, and changes in collagen conformation. As a result, examining cancer cell death with FTIR, which is both a cheap and fast method, can be used as an alternative method.

 Colon cancer is a common and highly lethal type of cancer all over the world. As with all cancers, monitoring the course of colon cancer is critical. 5-Fluorouracil 5-FU is an anticancer drug frequently used in colon cancers and causes some biochemical and molecular differences in cells during cell death. In this study, after incubation of HCT116 and HT29 colon cancer cells with 5-FU chemotherapy drug, Fourier transform infrared FTIR spectroscopy was used to detect the biochemical change caused by 5FU on cells. The concentration IC50 of 5-FU at which percent inhibition equals 50 was determined using the MTT method. FTIR measurements were taken after the IC50 value was determined. Accordingly, the IC50 value of 5-FU was found to be 12.69 µgml for HCT116 cells and 10.10 µgml for HT29 cells, respectively. FTIR spectroscopy showed changes in IR biomarkers such as the increase in lipid content, which is said to be indicators of cell death caused by 5-FU, and the nucleic acid ratio, the α-helix and β-sheet secondary structures of total cellular proteins, and the reduction of amino acid residues, and changes in collagen conformation. As a result, examining cancer cell death with FTIR, which is both a cheap and fast method, can be used as an alternative method.

 Kolon kanseri, tüm dünyada yaygın olan ve yüksek oranda ölümcül bir kanser tipidir. Tüm kanserlerde olduğu gibi kolon kanserinin seyrinin izlenmesi kritik bir öneme sahiptir. 5-Florourasil 5-FU kolon kanserlerinde sıklıkla kullanılan bir antikanser ilaçtır ve hücre ölümü esnasında hücrelerde bir takım biyokimyasal ve moleküler farklılıklara neden olmaktadır. Bu çalışmada HCT116 ve HT29 kolon kanseri hücreleri 5-FU kemoterapi ilacı ile inkübe edildikten sonra, 5FUnun hücreler üzerinde oluşturduğu biyokimyasal değişikliğin tespiti için Fourier transform kızılötesi FTIR spektroskopisi kullanılmıştır. 5-FUnun inhibisyon yüzdesinin 50ye eşit olduğu konsantrasyonu IC50, MTT yöntemi kullanılarak belirlendi. IC50 değeri saptandıktan sonra FTIR ölçümleri alındı. Buna göre, 5-FU IC50 değeri sırasıyla HCT116 hücreleri için 12,69 µgml, HT29 hücreleri için 10,10 µgml bulunmuştur. 5-FUnun sebep olduğu hücre ölümünün göstergeleri olduğu söylenen lipit içeriğindeki artış ile nükleik asit oranı, total hücresel proteinlerin α-sarmal ve β-yaprak ikincil yapıları ve aminoasit kalıntılarının azalması, kollajen konformasyonundaki değişiklikler gibi IR biyobelirteçlerindeki değişiklikler FTIR spektroskopisi ile gösterilmiştir. Sonuç olarak hem ucuz ve hem de hızlı bir yöntem olan FTIR ile kanser hücre ölümünün incelenmesi alternatif bir yöntem olarak kullanılabilir.

Makale detayları

Investigation of 5-Fluorouracil-Induced Cell Death in HCT116 and HT29 Colon Cancer Cells by Fourier Transform Infrared Spectroscopy

Yazarlar:

Dilşad Özerkan | Naci Tüzemen

Yayıncı:

Duzce University

Ana Başlıklar:

Mühendislik

Dergi:

Düzce Üniversitesi Bilim ve Teknoloji Dergisi Vol.11 Issue 1, pp.524-535

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001282903

pISSN:

2148-2446

Colon cancer

5-fluorouracil

Kolon kanseri

5-florourasil

FTIR

Colon cancer

5-fluorouracil

Sayı

Makale detayları

Yazarlar: Dilşad Özerkan | Naci Tüzemen

Yayıncı: Duzce University

Ana Başlıklar: Mühendislik

Dergi: Düzce Üniversitesi Bilim ve Teknoloji Dergisi Vol.11 Issue 1, pp.524-535

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: Türkçe

Document ID (Org No.): 2001282903

pISSN: 2148-2446

Sayı

Yazarlar:

Dilşad Özerkan | Naci Tüzemen

Yayıncı:

Duzce University

Ana Başlıklar:

Mühendislik

Dergi:

Düzce Üniversitesi Bilim ve Teknoloji Dergisi Vol.11 Issue 1, pp.524-535

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

Türkçe

Document ID (Org No.):

2001282903

pISSN:

2148-2446