eNOS Expression in the Cerebellum of Dogs Naturally Infected with Canine Distemper Virus and Realationship with Apoptosis

Mehmet Önder Karayiğit

Makale detayları

eNOS Expression in the Cerebellum of Dogs Naturally Infected with Canine Distemper Virus and Realationship with Apoptosis

Yazarlar:

Mehmet Önder Karayiğit

Yayıncı:

Balıkesir University

Ana Başlıklar:

Meyve Yetiştirme ve Islahı

Dergi:

Balıkesir Sağlık Bilimleri Dergisi Vol.13 Issue 2, pp.390-396

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325735

pISSN:

2146-9601

Anahtar kelimeler:

Özet:

 Objective: In this study, the role of eNOS expression in the neuropathology of canine distemper disease was investigated. Materials and Methods: In the study, cerebellar tissues of 20 dogs brought for necropsy and diagnosed with distemper by histopathological and immunohistochemical methods were used. Cerebellum tissues from 7 dogs that died of other diseases in which the central nervous system was not affected were used as controls. All tissues were fixed in 10 buffered formalin solution for 48 h then washed thoroughly in tap water overnight. After dehydration in graded alcohols, cleared in xylene and embedded in paraffin. Paraffin blocks of cerebellum were cut at 5 μm and stained with hematoxylin and eosin HE and Immunohistochemistry IHC was performed according to the manufacturers protocol. Results: Expression of eNOS and cells undergoing apoptosis in infected tissues were increased compared to control tissues. This difference was statistically significant. Conclusion: As a result, increased eNOS expression may be one of the factors that trigger apoptosis in neuropathology of canine distemper disease. However, further studies are needed for other effects that contribute to the neuropathology of the disease.

 Objective: In this study, the role of eNOS expression in the neuropathology of canine distemper disease was investigated. Materials and Methods: In the study, cerebellar tissues of 20 dogs brought for necropsy and diagnosed with distemper by histopathological and immunohistochemical methods were used. Cerebellum tissues from 7 dogs that died of other diseases in which the central nervous system was not affected were used as controls. All tissues were fixed in 10 buffered formalin solution for 48 h then washed thoroughly in tap water overnight. After dehydration in graded alcohols, cleared in xylene and embedded in paraffin. Paraffin blocks of cerebellum were cut at 5 μm and stained with hematoxylin and eosin HE and Immunohistochemistry IHC was performed according to the manufacturers protocol. Results: Expression of eNOS and cells undergoing apoptosis in infected tissues were increased compared to control tissues. This difference was statistically significant. Conclusion: As a result, increased eNOS expression may be one of the factors that trigger apoptosis in neuropathology of canine distemper disease. However, further studies are needed for other effects that contribute to the neuropathology of the disease.

 Objective: In this study, the role of eNOS expression in the neuropathology of canine distemper disease was investigated. Materials and Methods: In the study, cerebellar tissues of 20 dogs brought for necropsy and diagnosed with distemper by histopathological and immunohistochemical methods were used. Cerebellum tissues from 7 dogs that died of other diseases in which the central nervous system was not affected were used as controls. All tissues were fixed in 10 buffered formalin solution for 48 h then washed thoroughly in tap water overnight. After dehydration in graded alcohols, cleared in xylene and embedded in paraffin. Paraffin blocks of cerebellum were cut at 5 μm and stained with hematoxylin and eosin HE and Immunohistochemistry IHC was performed according to the manufacturers protocol. Results: Expression of eNOS and cells undergoing apoptosis in infected tissues were increased compared to control tissues. This difference was statistically significant. Conclusion: As a result, increased eNOS expression may be one of the factors that trigger apoptosis in neuropathology of canine distemper disease. However, further studies are needed for other effects that contribute to the neuropathology of the disease.

 Objective: In this study, the role of eNOS expression in the neuropathology of canine distemper disease was investigated. Materials and Methods: In the study, cerebellar tissues of 20 dogs brought for necropsy and diagnosed with distemper by histopathological and immunohistochemical methods were used. Cerebellum tissues from 7 dogs that died of other diseases in which the central nervous system was not affected were used as controls. All tissues were fixed in 10 buffered formalin solution for 48 h then washed thoroughly in tap water overnight. After dehydration in graded alcohols, cleared in xylene and embedded in paraffin. Paraffin blocks of cerebellum were cut at 5 μm and stained with hematoxylin and eosin HE and Immunohistochemistry IHC was performed according to the manufacturers protocol. Results: Expression of eNOS and cells undergoing apoptosis in infected tissues were increased compared to control tissues. This difference was statistically significant. Conclusion: As a result, increased eNOS expression may be one of the factors that trigger apoptosis in neuropathology of canine distemper disease. However, further studies are needed for other effects that contribute to the neuropathology of the disease.

Makale detayları

eNOS Expression in the Cerebellum of Dogs Naturally Infected with Canine Distemper Virus and Realationship with Apoptosis

Yazarlar:

Mehmet Önder Karayiğit

Yayıncı:

Balıkesir University

Ana Başlıklar:

Meyve Yetiştirme ve Islahı

Dergi:

Balıkesir Sağlık Bilimleri Dergisi Vol.13 Issue 2, pp.390-396

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325735

pISSN:

2146-9601

Apoptosis

Distemper

Dog

eNOS.

Sayı

Makale detayları

eNOS Expression in the Cerebellum of Dogs Naturally Infected with Canine Distemper Virus and Realationship with Apoptosis

Yazarlar: Mehmet Önder Karayiğit

Yayıncı: Balıkesir University

Ana Başlıklar: Meyve Yetiştirme ve Islahı

Dergi: Balıkesir Sağlık Bilimleri Dergisi Vol.13 Issue 2, pp.390-396

Belge Tipi: Makale

Yayın Tarihi: 8/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001325735

pISSN: 2146-9601

Apoptosis

Distemper

Dog

eNOS.

Sayı

Yazarlar:

Mehmet Önder Karayiğit

Yayıncı:

Balıkesir University

Ana Başlıklar:

Meyve Yetiştirme ve Islahı

Dergi:

Balıkesir Sağlık Bilimleri Dergisi Vol.13 Issue 2, pp.390-396

Belge Tipi:

Makale

Yayın Tarihi:

8/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001325735

pISSN:

2146-9601