Molecular characteristics and antibiotic resistance of Acinetobacter baumannii beta-lactamase-producing isolates, a predominance of intrinsic blaOXA-51, and detection of TEM and CTX-M genes

Amir IBRAHIMAGIC; Farah KAMBEROVIC; Selma UZUNOVIC; Branka BEDENIC; Emina IDRIZOVIC

Makale detayları

Molecular characteristics and antibiotic resistance of Acinetobacter baumannii beta-lactamase-producing isolates, a predominance of intrinsic blaOXA-51, and detection of TEM and CTX-M genes

Yazarlar:

Amir IBRAHIMAGIC | Farah KAMBEROVIC | Selma UZUNOVIC | Branka BEDENIC | Emina IDRIZOVIC

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 2, pp.715-720

Belge Tipi:

Makale

Yayın Tarihi:

3/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000751507

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144

Özet:

 

Background/aim: The aim of this study was to determine the molecular characteristics and antibiotic resistance of 13 (10 inpatient and  three outpatient) Acinetobacter baumannii beta-lactamase-producing isolates collected in Bosnia and Herzegovina between December  2009 and May 2010.  Materials and methods: Susceptibility testing was performed by disk diffusion and broth microdilution methods. The modified Hodge  and combined disk test with EDTA/phenylboronic acid was used to screen for carbapenemase production. Production of extended  spectrum beta-lactamases (ESBLs) was determined by double-disk synergy test. PCR was used to detect blaESBL/blacarb genes.  Results: Ten (22.2%) inpatient and three (13.6%) outpatient isolates produced beta-lactamases, ESBLs, or oxacillinases. More than 50%  of the isolates showed multidrug resistance. Resistance rates to gentamicin and ciprofloxacin of the inpatients and outpatients were  80.0%, 60.0%, 75.0%, and 25.0%, respectively. MICs of carbapenems for resistant isolates ranged from 32 to >256 μg/mL. All imipenemresistant  Acinetobacter baumannii strains contained blaOXA-51. Three of the 10 inpatient isolates and one outpatient isolate containing  blaOXA-51 additionally produced other beta-lactamases (TEM/CTX-M/OXA-1). None of the inpatient or outpatient isolates were positive  for other carbapenemases, especially acquired oxacillinases (blaOXA-23/blaOXA-24/blaOXA-58/blaOXA-143).  Conclusion: Production of blaOXA-51 presents an emerging threat in imipenem-resistant Acinetobacter spp. from Bosnia and Herzegovina.

Makale detayları

Molecular characteristics and antibiotic resistance of Acinetobacter baumannii beta-lactamase-producing isolates, a predominance of intrinsic blaOXA-51, and detection of TEM and CTX-M genes

Yazarlar: Amir IBRAHIMAGIC | Farah KAMBEROVIC | Selma UZUNOVIC | Branka BEDENIC | Emina IDRIZOVIC

Yayıncı: TUBITAK

Ana Başlıklar: Sağlık teşvikleri

Dergi: Turkish Journal of Medical Sciences 2017 Vol.47 Issue 2, pp.715-720

Belge Tipi: Makale

Yayın Tarihi: 3/2017

Belge Dili: İngilizce

Document ID (Org No.): 2000751507

Sıklık: İki Aylık

eISSN: 1303-6165

pISSN: 1300-0144

Sayı

Yazarlar:

Amir IBRAHIMAGIC | Farah KAMBEROVIC | Selma UZUNOVIC | Branka BEDENIC | Emina IDRIZOVIC

Yayıncı:

TUBITAK

Ana Başlıklar:

Sağlık teşvikleri

Dergi:

Turkish Journal of Medical Sciences 2017 Vol.47 Issue 2, pp.715-720

Belge Tipi:

Makale

Yayın Tarihi:

3/2017

Belge Dili:

İngilizce

Document ID (Org No.):

2000751507

Sıklık:

İki Aylık

eISSN:

1303-6165

pISSN:

1300-0144