Effects of Pharmaceuticals Used Against COVID-19 on HDAC Activity and Inflammation in Cell Line Obtained from a Colorectal Adenocarcinoma Patient

Hande Akalan

Makale detayları

Effects of Pharmaceuticals Used Against COVID-19 on HDAC Activity and Inflammation in Cell Line Obtained from a Colorectal Adenocarcinoma Patient

Yazarlar:

Hande Akalan

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Epigenetik | Hayvan Hücresi ve Moleküler Biyoloji

Dergi:

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.30 Issue 1, pp.1-12

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001344502

pISSN:

1300-5413

Anahtar kelimeler:

Özet:

 Different doses of various drugs were used in the treatment of the Coronavirus Disease 2019 COVID-19 pandemic that affected the whole world. Since these drugs are not directly related to COVID-19, it was very important to elucidate their effects. Studies have shown that COVID-19 is closely related to colorectal cancer. Our study examines the effects of Lopinavirritonavir, hydroxychloroquine, favipiravir, and oseltamivir, as well as their active ingredients used during COVID-19 treatment, on the histone deacetylase enzyme HDAC in colorectal cancer cells. Thus, it is aimed to provide a different perspective on the relationship between pandemic and colorectal cancer. In this study, the effects of the applied drugs on cell viability were evaluated with 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide MTT analysis, acridine orangepropidium iodide AOPI staining and histone deacetylase enzyme activity was evaluated in colorectal adenocarcinoma cell line Caco-2 with HDAC assay. Using an enzyme-linked immunosorbent assay ELISA, the inflammatory effects on the cells due to the application were assessed based on the expression levels of interleukin 10 IL-10, interleukin 6 IL-6, and tumor necrosis factor alpha TNF-α. Experimental groups showed continued cell viability, and an increase in HDAC enzyme activity was observed. This increase was found to effectively influence the IL-6 expression, which is believed to enhance inflammation.

 Different doses of various drugs were used in the treatment of the Coronavirus Disease 2019 COVID-19 pandemic that affected the whole world. Since these drugs are not directly related to COVID-19, it was very important to elucidate their effects. Studies have shown that COVID-19 is closely related to colorectal cancer. Our study examines the effects of Lopinavirritonavir, hydroxychloroquine, favipiravir, and oseltamivir, as well as their active ingredients used during COVID-19 treatment, on the histone deacetylase enzyme HDAC in colorectal cancer cells. Thus, it is aimed to provide a different perspective on the relationship between pandemic and colorectal cancer. In this study, the effects of the applied drugs on cell viability were evaluated with 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide MTT analysis, acridine orangepropidium iodide AOPI staining and histone deacetylase enzyme activity was evaluated in colorectal adenocarcinoma cell line Caco-2 with HDAC assay. Using an enzyme-linked immunosorbent assay ELISA, the inflammatory effects on the cells due to the application were assessed based on the expression levels of interleukin 10 IL-10, interleukin 6 IL-6, and tumor necrosis factor alpha TNF-α. Experimental groups showed continued cell viability, and an increase in HDAC enzyme activity was observed. This increase was found to effectively influence the IL-6 expression, which is believed to enhance inflammation.

 Different doses of various drugs were used in the treatment of the Coronavirus Disease 2019 COVID-19 pandemic that affected the whole world. Since these drugs are not directly related to COVID-19, it was very important to elucidate their effects. Studies have shown that COVID-19 is closely related to colorectal cancer. Our study examines the effects of Lopinavirritonavir, hydroxychloroquine, favipiravir, and oseltamivir, as well as their active ingredients used during COVID-19 treatment, on the histone deacetylase enzyme HDAC in colorectal cancer cells. Thus, it is aimed to provide a different perspective on the relationship between pandemic and colorectal cancer. In this study, the effects of the applied drugs on cell viability were evaluated with 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide MTT analysis, acridine orangepropidium iodide AOPI staining and histone deacetylase enzyme activity was evaluated in colorectal adenocarcinoma cell line Caco-2 with HDAC assay. Using an enzyme-linked immunosorbent assay ELISA, the inflammatory effects on the cells due to the application were assessed based on the expression levels of interleukin 10 IL-10, interleukin 6 IL-6, and tumor necrosis factor alpha TNF-α. Experimental groups showed continued cell viability, and an increase in HDAC enzyme activity was observed. This increase was found to effectively influence the IL-6 expression, which is believed to enhance inflammation.

 Koronavirüs Hastalığı 2019 COVID-19 pandemisinin tedavisinde çeşitli ilaçların farklı dozları kullanıldı. Bu ilaçlar doğrudan COVID-19 ile ilişkili olmadığından oluşturacakları etkilerin aydınlatılması oldukça önem taşımaktaydı. Yapılan araştırmalarla COVID-19un kolorektal kanser ile yakından ilişkili olduğu ortaya konulmuştur. Gerçekleştirdiğimiz çalışmada kolorektal kanser hücre soyunda Caco-2, COVID-19 tedavisinde kullanılan lopinavirritonavir, hidroksiklorokin, favipiravir, oseltamivir ilaç ve etken maddelerinin histon deasetilaz enzimi HDAC üzerindeki etkinlikleri ve enflamasyona katkıları araştırılmıştır. Böylece pandeminin kolorektal kanserle ilişkisine farklı bir bakış açısı kazandırmış olmak hedeflenmiştir. Bu çalışmada, uygulanan etken maddelerin Caco-2 hücrelerinin hücre canlılığı üzerindeki etkileri; 3-4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromid MTT analizi, akridin turuncusupropidyum iyodür AOPI boyaması kullanılarak belirlendi ve histon deasetilaz enzim aktivitesi HDAC testi ile saptandı. Ayrıca enflamatuvar etki, enzim bağlantılı immünosorbent testi ELISA kullanılarak interlökin 10 IL-10, interlökin 6 IL-6 ve tümör nekroz faktörü alfa TNF-α ekspresyon seviyelerinde meydana gelen değişiklere göre değerlendirildi. Uygulama yapılan gruplarda hücre canlılığı devam ederken, HDAC enzim aktivitesinde artış meydana geldi. Bu artışın IL-6 ekspresyonu üzerinde etkili olduğu gösterilerek enflamasyona neden olduğu düşünülmektedir.

Makale detayları

Effects of Pharmaceuticals Used Against COVID-19 on HDAC Activity and Inflammation in Cell Line Obtained from a Colorectal Adenocarcinoma Patient

Yazarlar:

Hande Akalan

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Epigenetik | Hayvan Hücresi ve Moleküler Biyoloji

Dergi:

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.30 Issue 1, pp.1-12

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001344502

pISSN:

1300-5413

Favipiravir

HDAC

Hydroxychloroquine

Lopinavir/ritonavir

Oseltamivir

Valproic acidFavipiravir

HDAC

Hidroksiklorokin

Lopinavir/ritonavir

Oseltamivir

Valproik asit

Sayı

Makale detayları

Yazarlar: Hande Akalan

Yayıncı: Van Yuzuncu Yıl University

Ana Başlıklar: Epigenetik | Hayvan Hücresi ve Moleküler Biyoloji

Dergi: Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.30 Issue 1, pp.1-12

Belge Tipi: Makale

Yayın Tarihi: 4/2025

Belge Dili: İngilizce

Document ID (Org No.): 2001344502

pISSN: 1300-5413

Sayı

Yazarlar:

Hande Akalan

Yayıncı:

Van Yuzuncu Yıl University

Ana Başlıklar:

Epigenetik | Hayvan Hücresi ve Moleküler Biyoloji

Dergi:

Yüzüncü Yıl Üniversitesi Fen Bilimleri Enstitüsü Dergisi Vol.30 Issue 1, pp.1-12

Belge Tipi:

Makale

Yayın Tarihi:

4/2025

Belge Dili:

İngilizce

Document ID (Org No.):

2001344502

pISSN:

1300-5413