Evaluation of immunochromatography method in the diagnosis of cystic echinococcosis

Özlem Koca; Özgül Çetinkaya; Aylin Erman Daloğlu; Yeşim Çekin; Hatice Özen

Makale detayları

Evaluation of immunochromatography method in the diagnosis of cystic echinococcosis

Yazarlar:

Özlem Koca | Özgül Çetinkaya | Aylin Erman Daloğlu | Yeşim Çekin | Hatice Özen

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Tıbbi Parazitoloji

Dergi:

Anatolian Current Medical Journal Vol.5 Issue 4, pp.389-394

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302551

pISSN:

2718-0115

Anahtar kelimeler:

Özet:

 Aims: Echinococcus granulosus is the causative agent of hydatid cyst, or cystic echinococcosis CE, with its current name. Echinococcus granulosus is a zoonotic cestode; commonly found in humans and farm animals. In cystic echinococcosis infection, transmission occurs by oral ingestion of parasite eggs excreted in infected dog feces. The larval form is responsible for the formation of slowly growing cysts in the organs and tissues of mammals such as humans, sheep, goats and cattle. In this study, it was aimed to compare indirect hemagglutination IHA and immunochromatographic ICT methods from the sera of patients with suspected CE and to evaluate serological tests based on imaging and clinical diagnosis. Methods: Between 31 October 2022 and 31 January 2023, blood samples of 95 patients with suspected CE from different units of our hospital and for whom IHA was routinely requested were included in the study prospectively. VIRAPID Hydatidosis Vircell, Granada, Spain test using the immunochromatographic method and ELI.H.A. Echinococcus ELITech Microbio, France test was studied in accordance with the manufacturers instructions. Results: Based on clinical and imaging methods of 95 patients included in our study, 64 63.1 were diagnosed with hydatid cyst. Based on imaging and clinical diagnosis; sensitivity, specificity, positive predictive value and negative predictive values were calculated as 81.3, 96.8, 98.1, 71.4 for the IHA test, and were calculated as 75.0, 93.5, 96.6, 64.4 for the ICT test, respectively. Good agreement was found between the two tests percent agreement68.0; kappa value0.682; p0.001. Sensitivity IHA: 87.2; ICT: 94.9 and specificity IHA: 96.8; ICT: 93.5 of IHA positive titer of 1160 and above and ICT methods in active cysts with cyst stage CE 1-2-3 values were found to be compatible, The sensitivity of the ICT method in inactive cysts with CE 4-5 IHA: 72, ICA: 44 was found to be statistically significantly lower than the IHA method p0.001. Conclusion: Rapid diagnostic tests generally stand out as they do not require personnel training in health institutions and are easy to apply. Especially in the active period of the cysts, the tests show a very good and harmonious performance, and it significantly supports the clinical and radiological findings in the early diagnosis of the disease and in the treatment follow-up, however, they need to be developed in order to be used in the differential diagnosis of inactive cyst stages, especially in cases in between, and performance studies in larger patient groups are required.

 Aims: Echinococcus granulosus is the causative agent of hydatid cyst, or cystic echinococcosis CE, with its current name. Echinococcus granulosus is a zoonotic cestode; commonly found in humans and farm animals. In cystic echinococcosis infection, transmission occurs by oral ingestion of parasite eggs excreted in infected dog feces. The larval form is responsible for the formation of slowly growing cysts in the organs and tissues of mammals such as humans, sheep, goats and cattle. In this study, it was aimed to compare indirect hemagglutination IHA and immunochromatographic ICT methods from the sera of patients with suspected CE and to evaluate serological tests based on imaging and clinical diagnosis. Methods: Between 31 October 2022 and 31 January 2023, blood samples of 95 patients with suspected CE from different units of our hospital and for whom IHA was routinely requested were included in the study prospectively. VIRAPID Hydatidosis Vircell, Granada, Spain test using the immunochromatographic method and ELI.H.A. Echinococcus ELITech Microbio, France test was studied in accordance with the manufacturers instructions. Results: Based on clinical and imaging methods of 95 patients included in our study, 64 63.1 were diagnosed with hydatid cyst. Based on imaging and clinical diagnosis; sensitivity, specificity, positive predictive value and negative predictive values were calculated as 81.3, 96.8, 98.1, 71.4 for the IHA test, and were calculated as 75.0, 93.5, 96.6, 64.4 for the ICT test, respectively. Good agreement was found between the two tests percent agreement68.0; kappa value0.682; p0.001. Sensitivity IHA: 87.2; ICT: 94.9 and specificity IHA: 96.8; ICT: 93.5 of IHA positive titer of 1160 and above and ICT methods in active cysts with cyst stage CE 1-2-3 values were found to be compatible, The sensitivity of the ICT method in inactive cysts with CE 4-5 IHA: 72, ICA: 44 was found to be statistically significantly lower than the IHA method p0.001. Conclusion: Rapid diagnostic tests generally stand out as they do not require personnel training in health institutions and are easy to apply. Especially in the active period of the cysts, the tests show a very good and harmonious performance, and it significantly supports the clinical and radiological findings in the early diagnosis of the disease and in the treatment follow-up, however, they need to be developed in order to be used in the differential diagnosis of inactive cyst stages, especially in cases in between, and performance studies in larger patient groups are required.

 Aims: Echinococcus granulosus is the causative agent of hydatid cyst, or cystic echinococcosis CE, with its current name. Echinococcus granulosus is a zoonotic cestode; commonly found in humans and farm animals. In cystic echinococcosis infection, transmission occurs by oral ingestion of parasite eggs excreted in infected dog feces. The larval form is responsible for the formation of slowly growing cysts in the organs and tissues of mammals such as humans, sheep, goats and cattle. In this study, it was aimed to compare indirect hemagglutination IHA and immunochromatographic ICT methods from the sera of patients with suspected CE and to evaluate serological tests based on imaging and clinical diagnosis. Methods: Between 31 October 2022 and 31 January 2023, blood samples of 95 patients with suspected CE from different units of our hospital and for whom IHA was routinely requested were included in the study prospectively. VIRAPID Hydatidosis Vircell, Granada, Spain test using the immunochromatographic method and ELI.H.A. Echinococcus ELITech Microbio, France test was studied in accordance with the manufacturers instructions. Results: Based on clinical and imaging methods of 95 patients included in our study, 64 63.1 were diagnosed with hydatid cyst. Based on imaging and clinical diagnosis; sensitivity, specificity, positive predictive value and negative predictive values were calculated as 81.3, 96.8, 98.1, 71.4 for the IHA test, and were calculated as 75.0, 93.5, 96.6, 64.4 for the ICT test, respectively. Good agreement was found between the two tests percent agreement68.0; kappa value0.682; p0.001. Sensitivity IHA: 87.2; ICT: 94.9 and specificity IHA: 96.8; ICT: 93.5 of IHA positive titer of 1160 and above and ICT methods in active cysts with cyst stage CE 1-2-3 values were found to be compatible, The sensitivity of the ICT method in inactive cysts with CE 4-5 IHA: 72, ICA: 44 was found to be statistically significantly lower than the IHA method p0.001. Conclusion: Rapid diagnostic tests generally stand out as they do not require personnel training in health institutions and are easy to apply. Especially in the active period of the cysts, the tests show a very good and harmonious performance, and it significantly supports the clinical and radiological findings in the early diagnosis of the disease and in the treatment follow-up, however, they need to be developed in order to be used in the differential diagnosis of inactive cyst stages, especially in cases in between, and performance studies in larger patient groups are required.

 Aims: Echinococcus granulosus is the causative agent of hydatid cyst, or cystic echinococcosis CE, with its current name. Echinococcus granulosus is a zoonotic cestode; commonly found in humans and farm animals. In cystic echinococcosis infection, transmission occurs by oral ingestion of parasite eggs excreted in infected dog feces. The larval form is responsible for the formation of slowly growing cysts in the organs and tissues of mammals such as humans, sheep, goats and cattle. In this study, it was aimed to compare indirect hemagglutination IHA and immunochromatographic ICT methods from the sera of patients with suspected CE and to evaluate serological tests based on imaging and clinical diagnosis. Methods: Between 31 October 2022 and 31 January 2023, blood samples of 95 patients with suspected CE from different units of our hospital and for whom IHA was routinely requested were included in the study prospectively. VIRAPID Hydatidosis Vircell, Granada, Spain test using the immunochromatographic method and ELI.H.A. Echinococcus ELITech Microbio, France test was studied in accordance with the manufacturers instructions. Results: Based on clinical and imaging methods of 95 patients included in our study, 64 63.1 were diagnosed with hydatid cyst. Based on imaging and clinical diagnosis; sensitivity, specificity, positive predictive value and negative predictive values were calculated as 81.3, 96.8, 98.1, 71.4 for the IHA test, and were calculated as 75.0, 93.5, 96.6, 64.4 for the ICT test, respectively. Good agreement was found between the two tests percent agreement68.0; kappa value0.682; p0.001. Sensitivity IHA: 87.2; ICT: 94.9 and specificity IHA: 96.8; ICT: 93.5 of IHA positive titer of 1160 and above and ICT methods in active cysts with cyst stage CE 1-2-3 values were found to be compatible, The sensitivity of the ICT method in inactive cysts with CE 4-5 IHA: 72, ICA: 44 was found to be statistically significantly lower than the IHA method p0.001. Conclusion: Rapid diagnostic tests generally stand out as they do not require personnel training in health institutions and are easy to apply. Especially in the active period of the cysts, the tests show a very good and harmonious performance, and it significantly supports the clinical and radiological findings in the early diagnosis of the disease and in the treatment follow-up, however, they need to be developed in order to be used in the differential diagnosis of inactive cyst stages, especially in cases in between, and performance studies in larger patient groups are required.

Makale detayları

Evaluation of immunochromatography method in the diagnosis of cystic echinococcosis

Yazarlar:

Özlem Koca | Özgül Çetinkaya | Aylin Erman Daloğlu | Yeşim Çekin | Hatice Özen

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Tıbbi Parazitoloji

Dergi:

Anatolian Current Medical Journal Vol.5 Issue 4, pp.389-394

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302551

pISSN:

2718-0115

Cystic echinococcosis

Echinococcus granulosus

immunochromatography

indirect hemagglutination

Sayı

Makale detayları

Evaluation of immunochromatography method in the diagnosis of cystic echinococcosis

Yazarlar: Özlem Koca | Özgül Çetinkaya | Aylin Erman Daloğlu | Yeşim Çekin | Hatice Özen

Yayıncı: MediHealth Academy Yayıncılık

Ana Başlıklar: Tıbbi Parazitoloji

Dergi: Anatolian Current Medical Journal Vol.5 Issue 4, pp.389-394

Belge Tipi: Makale

Yayın Tarihi: 10/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001302551

pISSN: 2718-0115

Cystic echinococcosis

Echinococcus granulosus

immunochromatography

indirect hemagglutination

Sayı

Yazarlar:

Özlem Koca | Özgül Çetinkaya | Aylin Erman Daloğlu | Yeşim Çekin | Hatice Özen

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Tıbbi Parazitoloji

Dergi:

Anatolian Current Medical Journal Vol.5 Issue 4, pp.389-394

Belge Tipi:

Makale

Yayın Tarihi:

10/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001302551

pISSN:

2718-0115