Does ramelteon have an ameliorative effect in MTX-induced testicular injury?

Dilek Ulusoy Karatopuk; Nureddin Atasoy; Duygu Kumbul Doğuç

Makale detayları

Does ramelteon have an ameliorative effect in MTX-induced testicular injury?

Yazarlar:

Dilek Ulusoy Karatopuk | Nureddin Atasoy | Duygu Kumbul Doğuç

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Histoloji ve Embriyoloji

Dergi:

Journal of Health Sciences and Medicine Vol.6 Issue 5, pp.1034-1039

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299339

pISSN:

2636-8579

Anahtar kelimeler:

Özet:

 Aims: The aim of this study was to investigate the potential protective effect of Ramelteon RMT, which exhibits antioxidant, anti-inflammatory and antiapoptotic properties, against testicular damage induced by Methotrexate MTX, which is widely used in the treatment of various diseases, including chemotherapy. Methods: 32 Wistar albino rats were equally divided into four groups: Control group I, MTX group II, MTXRMT group III and RMT group IV. Histologic evaluation was performed using Hematoxylin and Eosin HE staining, immunohistochemical analysis using TNF-alpha and Cas-3, and biochemical evaluation using TAS, TOS and OSI. Results: Histologic analysis using HE staining revealed a significant difference between group I and groups II and III p0.05, while no significant difference was observed between group IV and the other groups p0.05. While normal histologic structures were observed in groups I and IV, histopathologic findings were noted in groups II and III. Immunohistochemical evaluation of TNF-alpha and Cas-3 showed a significant difference between group I and groups II and III p0.05, while no significant difference was observed between group IV and other groups p0.05. The highest immunostaining intensity was observed in group II. Biochemical evaluation revealed statistically significant differences in TAS, TOS and OSI parameters reflecting oxidative stress differences between the groups p0.05. Conclusion: Anti-inflammatory, antioxidant and antiapoptotic properties of RMT demonstrated its protective effect against MTX-induced testicular injury in rats. Histological, immunohistochemical and biochemical analyses underline the potential role of RMT as a protective agent in MTX-induced testicular injury. This research aims to contribute to the understanding of potential applications to reduce the adverse effects of MTX therapy on reproductive health.

 Aims: The aim of this study was to investigate the potential protective effect of Ramelteon RMT, which exhibits antioxidant, anti-inflammatory and antiapoptotic properties, against testicular damage induced by Methotrexate MTX, which is widely used in the treatment of various diseases, including chemotherapy. Methods: 32 Wistar albino rats were equally divided into four groups: Control group I, MTX group II, MTXRMT group III and RMT group IV. Histologic evaluation was performed using Hematoxylin and Eosin HE staining, immunohistochemical analysis using TNF-alpha and Cas-3, and biochemical evaluation using TAS, TOS and OSI. Results: Histologic analysis using HE staining revealed a significant difference between group I and groups II and III p0.05, while no significant difference was observed between group IV and the other groups p0.05. While normal histologic structures were observed in groups I and IV, histopathologic findings were noted in groups II and III. Immunohistochemical evaluation of TNF-alpha and Cas-3 showed a significant difference between group I and groups II and III p0.05, while no significant difference was observed between group IV and other groups p0.05. The highest immunostaining intensity was observed in group II. Biochemical evaluation revealed statistically significant differences in TAS, TOS and OSI parameters reflecting oxidative stress differences between the groups p0.05. Conclusion: Anti-inflammatory, antioxidant and antiapoptotic properties of RMT demonstrated its protective effect against MTX-induced testicular injury in rats. Histological, immunohistochemical and biochemical analyses underline the potential role of RMT as a protective agent in MTX-induced testicular injury. This research aims to contribute to the understanding of potential applications to reduce the adverse effects of MTX therapy on reproductive health.

 Aims: The aim of this study was to investigate the potential protective effect of Ramelteon RMT, which exhibits antioxidant, anti-inflammatory and antiapoptotic properties, against testicular damage induced by Methotrexate MTX, which is widely used in the treatment of various diseases, including chemotherapy. Methods: 32 Wistar albino rats were equally divided into four groups: Control group I, MTX group II, MTXRMT group III and RMT group IV. Histologic evaluation was performed using Hematoxylin and Eosin HE staining, immunohistochemical analysis using TNF-alpha and Cas-3, and biochemical evaluation using TAS, TOS and OSI. Results: Histologic analysis using HE staining revealed a significant difference between group I and groups II and III p0.05, while no significant difference was observed between group IV and the other groups p0.05. While normal histologic structures were observed in groups I and IV, histopathologic findings were noted in groups II and III. Immunohistochemical evaluation of TNF-alpha and Cas-3 showed a significant difference between group I and groups II and III p0.05, while no significant difference was observed between group IV and other groups p0.05. The highest immunostaining intensity was observed in group II. Biochemical evaluation revealed statistically significant differences in TAS, TOS and OSI parameters reflecting oxidative stress differences between the groups p0.05. Conclusion: Anti-inflammatory, antioxidant and antiapoptotic properties of RMT demonstrated its protective effect against MTX-induced testicular injury in rats. Histological, immunohistochemical and biochemical analyses underline the potential role of RMT as a protective agent in MTX-induced testicular injury. This research aims to contribute to the understanding of potential applications to reduce the adverse effects of MTX therapy on reproductive health.

 Aims: The aim of this study was to investigate the potential protective effect of Ramelteon RMT, which exhibits antioxidant, anti-inflammatory and antiapoptotic properties, against testicular damage induced by Methotrexate MTX, which is widely used in the treatment of various diseases, including chemotherapy. Methods: 32 Wistar albino rats were equally divided into four groups: Control group I, MTX group II, MTXRMT group III and RMT group IV. Histologic evaluation was performed using Hematoxylin and Eosin HE staining, immunohistochemical analysis using TNF-alpha and Cas-3, and biochemical evaluation using TAS, TOS and OSI. Results: Histologic analysis using HE staining revealed a significant difference between group I and groups II and III p0.05, while no significant difference was observed between group IV and the other groups p0.05. While normal histologic structures were observed in groups I and IV, histopathologic findings were noted in groups II and III. Immunohistochemical evaluation of TNF-alpha and Cas-3 showed a significant difference between group I and groups II and III p0.05, while no significant difference was observed between group IV and other groups p0.05. The highest immunostaining intensity was observed in group II. Biochemical evaluation revealed statistically significant differences in TAS, TOS and OSI parameters reflecting oxidative stress differences between the groups p0.05. Conclusion: Anti-inflammatory, antioxidant and antiapoptotic properties of RMT demonstrated its protective effect against MTX-induced testicular injury in rats. Histological, immunohistochemical and biochemical analyses underline the potential role of RMT as a protective agent in MTX-induced testicular injury. This research aims to contribute to the understanding of potential applications to reduce the adverse effects of MTX therapy on reproductive health.

Makale detayları

Does ramelteon have an ameliorative effect in MTX-induced testicular injury?

Yazarlar: Dilek Ulusoy Karatopuk | Nureddin Atasoy | Duygu Kumbul Doğuç

Yayıncı: MediHealth Academy Yayıncılık

Ana Başlıklar: Histoloji ve Embriyoloji

Dergi: Journal of Health Sciences and Medicine Vol.6 Issue 5, pp.1034-1039

Belge Tipi: Makale

Yayın Tarihi: 9/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001299339

pISSN: 2636-8579

Methotrexate

ramelteon

rat

testicular injury

Sayı

Yazarlar:

Dilek Ulusoy Karatopuk | Nureddin Atasoy | Duygu Kumbul Doğuç

Yayıncı:

MediHealth Academy Yayıncılık

Ana Başlıklar:

Histoloji ve Embriyoloji

Dergi:

Journal of Health Sciences and Medicine Vol.6 Issue 5, pp.1034-1039

Belge Tipi:

Makale

Yayın Tarihi:

9/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001299339

pISSN:

2636-8579