Identification and evolutionary genomics of novel LTR retrotransposons in Brassica

FAISAL NOUROZ; SHUMAILA NOREEN; JOHN SEYMOUR HESLOP-HARRISON

Makale detayları

Identification and evolutionary genomics of novel LTR retrotransposons in Brassica

Yazarlar:

FAISAL NOUROZ | SHUMAILA NOREEN | JOHN SEYMOUR HESLOP-HARRISON

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.740-757

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811854

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Abstract: Retrotransposons (REs) are the most abundant and diverse elements identified from eukaryotic genomes. Using computational and molecular methods, 262 intact LTR retrotransposons were identified from Brassica genomes by dot plot analysis and data mining. The Copia superfamily was dominant (206 elements) over Gypsy (56), with estimated intact copies of ~1596 Copia and 540 Gypsy and ~7540 Copia and 780 Gypsy from Brassica rapa and Brassica oleracea whole genomes, respectively. Canonical Copia and Gypsy gag-pol polyprotein organizations were observed in most elements with a few displaying 1-3 additional or internally deleted domains. The PBS and PPT motifs were identified with tRNA complementary to tRNAMet or, rarely, other tRNA types. PCR amplification of RT regions revealed their abundance and distribution among A-, B-, and C-genome Brassicas indicating a common ancestor. The evolutionary relationship of Brassica REs resolved them into superfamily-specific (Copia/Gypsy) lineages. The phylogenetic analysis of 130 Brassica Copia clustered them into 2 clades and 10 sub-clades of 18 families; Gypsy elements clustered into 2 clades. The results enabled identification and understanding of the structure and nature of full-length REs and their derivatives in Brassica. The markers derived here will be useful for examining chromosome and genome evolution in Brassica.

 

Abstract: Retrotransposons (REs) are the most abundant and diverse elements identified from eukaryotic genomes. Using computational and molecular methods, 262 intact LTR retrotransposons were identified from Brassica genomes by dot plot analysis and data mining. The Copia superfamily was dominant (206 elements) over Gypsy (56), with estimated intact copies of ~1596 Copia and 540 Gypsy and ~7540 Copia and 780 Gypsy from Brassica rapa and Brassica oleracea whole genomes, respectively. Canonical Copia and Gypsy gag-pol polyprotein organizations were observed in most elements with a few displaying 1-3 additional or internally deleted domains. The PBS and PPT motifs were identified with tRNA complementary to tRNAMet or, rarely, other tRNA types. PCR amplification of RT regions revealed their abundance and distribution among A-, B-, and C-genome Brassicas indicating a common ancestor. The evolutionary relationship of Brassica REs resolved them into superfamily-specific (Copia/Gypsy) lineages. The phylogenetic analysis of 130 Brassica Copia clustered them into 2 clades and 10 sub-clades of 18 families; Gypsy elements clustered into 2 clades. The results enabled identification and understanding of the structure and nature of full-length REs and their derivatives in Brassica. The markers derived here will be useful for examining chromosome and genome evolution in Brassica.

Makale detayları

Identification and evolutionary genomics of novel LTR retrotransposons in Brassica

Yazarlar:

FAISAL NOUROZ | SHUMAILA NOREEN | JOHN SEYMOUR HESLOP-HARRISON

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.740-757

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811854

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

LTR retrotransposons

Brassica

Copia

Gypsy

evolutionary relationship

RTAP markers

Sayı

Makale detayları

Identification and evolutionary genomics of novel LTR retrotransposons in Brassica

Yazarlar: FAISAL NOUROZ | SHUMAILA NOREEN | JOHN SEYMOUR HESLOP-HARRISON

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.740-757

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811854

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

LTR retrotransposons

Brassica

Copia

Gypsy

evolutionary relationship

RTAP markers

Sayı

Yazarlar:

FAISAL NOUROZ | SHUMAILA NOREEN | JOHN SEYMOUR HESLOP-HARRISON

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 5, pp.740-757

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811854

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152