Phytofabrication of biomolecule-coated metallic silver nanoparticles using leaf extracts of in vitro-raised bamboo species and its anticancer activity against human PC3 cell lines

KALAMEGAM KALAIARASI; GOVINDARAJ PRASANNARAJ; SHIVENDRA VIKRAM SAHI; PERUMAL VENKATACHALAM

Makale detayları

Phytofabrication of biomolecule-coated metallic silver nanoparticles using leaf extracts of in vitro-raised bamboo species and its anticancer activity against human PC3 cell lines

Yazarlar:

KALAMEGAM KALAIARASI | GOVINDARAJ PRASANNARAJ | SHIVENDRA VIKRAM SAHI | PERUMAL VENKATACHALAM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 2, pp.223-232

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811967

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

The present study describes the biosynthesis of metallic silver nanoparticles (AgNPs) from silver ions using in vitro-grown leaf samples of Bambusa arundinacea (Ba) and Bambusa nutans (Bn) and their anticancer activity against human prostatic cancer cell lines (PC3). The metallic AgNPs were synthesized at room temperature by treating the leaf extracts of Bambusa arundinacea and Bambusa nutans with 2 mM silver nitrate using the boiled method. The characterization of synthesized BaAgNPs and BnAgNPs was performed using UV-Vis spectrum (absorption peak at 365 nm), FTIR, TEM (spherical shape), SEM (size of 30-90 nm), and EDX analysis. Cell viability assays were carried out to determine the cytotoxicity effect of AgNPs on the PC3 cancer cell line and the Vero normal cell line. The IC_50 values for BaAgNP- and BnAgNP-treated PC3 cancer cells were found to be 73.57 µg/mL and 84.88 µg/mL while the values for treated Vero cells were found to be 93.58 µg/mL and 96.41 µg/mL, respectively. The percentages of the apoptotic bodies by AO/EtBr staining were found to be 76% and 62% for BaAgNPs and BnAgNPs, respectively. The results strongly suggest that synthesized BaAgNPs show potential anticancer activity against human PC3 cell lines compared to BnAgNPs.

 

The present study describes the biosynthesis of metallic silver nanoparticles (AgNPs) from silver ions using in vitro-grown leaf samples of Bambusa arundinacea (Ba) and Bambusa nutans (Bn) and their anticancer activity against human prostatic cancer cell lines (PC3). The metallic AgNPs were synthesized at room temperature by treating the leaf extracts of Bambusa arundinacea and Bambusa nutans with 2 mM silver nitrate using the boiled method. The characterization of synthesized BaAgNPs and BnAgNPs was performed using UV-Vis spectrum (absorption peak at 365 nm), FTIR, TEM (spherical shape), SEM (size of 30-90 nm), and EDX analysis. Cell viability assays were carried out to determine the cytotoxicity effect of AgNPs on the PC3 cancer cell line and the Vero normal cell line. The IC_50 values for BaAgNP- and BnAgNP-treated PC3 cancer cells were found to be 73.57 µg/mL and 84.88 µg/mL while the values for treated Vero cells were found to be 93.58 µg/mL and 96.41 µg/mL, respectively. The percentages of the apoptotic bodies by AO/EtBr staining were found to be 76% and 62% for BaAgNPs and BnAgNPs, respectively. The results strongly suggest that synthesized BaAgNPs show potential anticancer activity against human PC3 cell lines compared to BnAgNPs.

Makale detayları

Phytofabrication of biomolecule-coated metallic silver nanoparticles using leaf extracts of in vitro-raised bamboo species and its anticancer activity against human PC3 cell lines

Yazarlar:

KALAMEGAM KALAIARASI | GOVINDARAJ PRASANNARAJ | SHIVENDRA VIKRAM SAHI | PERUMAL VENKATACHALAM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 2, pp.223-232

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811967

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anticancer activity

biological synthesis

bamboo

silver nanoparticles

electron microscopy

PC3 human prostate cancer cell lines

Sayı

Makale detayları

Phytofabrication of biomolecule-coated metallic silver nanoparticles using leaf extracts of in vitro-raised bamboo species and its anticancer activity against human PC3 cell lines

Yazarlar: KALAMEGAM KALAIARASI | GOVINDARAJ PRASANNARAJ | SHIVENDRA VIKRAM SAHI | PERUMAL VENKATACHALAM

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2015 Vol.39 Issue 2, pp.223-232

Belge Tipi: Makale

Yayın Tarihi: 8/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000811967

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Anticancer activity

biological synthesis

bamboo

silver nanoparticles

electron microscopy

PC3 human prostate cancer cell lines

Sayı

Yazarlar:

KALAMEGAM KALAIARASI | GOVINDARAJ PRASANNARAJ | SHIVENDRA VIKRAM SAHI | PERUMAL VENKATACHALAM

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2015 Vol.39 Issue 2, pp.223-232

Belge Tipi:

Makale

Yayın Tarihi:

8/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000811967

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152