Exploring the Bacterial Diversity of the Armutlu Geothermal Spring (Türkiye) through a Metagenomic Sequenced Based Approach

Anıl Cebeci; Merve Öztuğ; Hande Mumcu; Nevin Gül Karagüler

Makale detayları

Exploring the Bacterial Diversity of the Armutlu Geothermal Spring (Türkiye) through a Metagenomic Sequenced Based Approach

Yazarlar:

Anıl Cebeci | Merve Öztuğ | Hande Mumcu | Nevin Gül Karagüler

Yayıncı:

Yozgat Bozok University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Bozok Journal of Science Vol.1 Issue 2, pp.57-64

Belge Tipi:

Makale

Yayın Tarihi:

11/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001304429

pISSN:

2980-3314

Anahtar kelimeler:

Özet:

 The geothermal or hot springs are home to a diverse microbial population. Identification of the thermophilic microbial world of these niches can direct us to the exploration of novel economically valuable biocatalysts, antibiotics, and a variety of other biomolecules. Due to the tectonic features within its borders, Türkiye has many thermal springs in different regions. In this study, a thermal site, the Armutlu geothermal spring in the eastern Marmara region, was investigated to identify the bacterial taxonomic composition and diversity through a metagenomic sequence-based approach which is the first study to the best of our knowledge. During the period of sampling, the water temperature and pH at the surface were 74.9 C and 7.1, respectively. According to 16S rRNA sequencing results, Bacteria 98.36 are the dominant taxa compared to Archaea 1.64. Based on the diversity findings, the bacterial community is primarily dominated by Proteobacteria 42.49, which, together with Chloroflexi, Acidobacteria, Actinobacteria, Firmicutes, Nitrospirae, and Bacteroidetes, constitute 87 of the overall bacterial population. Furthermore, the microbial community was also investigated for the activity of hydrolase enzymes such as protease, amylase, and amylopullulanase through a culture-dependent approach for possible future applications. As a conclusion, the microbiota of the Armutlu geothermal spring was open to investigate the novel species and hydrolytic biocatalysts.

 The geothermal or hot springs are home to a diverse microbial population. Identification of the thermophilic microbial world of these niches can direct us to the exploration of novel economically valuable biocatalysts, antibiotics, and a variety of other biomolecules. Due to the tectonic features within its borders, Türkiye has many thermal springs in different regions. In this study, a thermal site, the Armutlu geothermal spring in the eastern Marmara region, was investigated to identify the bacterial taxonomic composition and diversity through a metagenomic sequence-based approach which is the first study to the best of our knowledge. During the period of sampling, the water temperature and pH at the surface were 74.9 C and 7.1, respectively. According to 16S rRNA sequencing results, Bacteria 98.36 are the dominant taxa compared to Archaea 1.64. Based on the diversity findings, the bacterial community is primarily dominated by Proteobacteria 42.49, which, together with Chloroflexi, Acidobacteria, Actinobacteria, Firmicutes, Nitrospirae, and Bacteroidetes, constitute 87 of the overall bacterial population. Furthermore, the microbial community was also investigated for the activity of hydrolase enzymes such as protease, amylase, and amylopullulanase through a culture-dependent approach for possible future applications. As a conclusion, the microbiota of the Armutlu geothermal spring was open to investigate the novel species and hydrolytic biocatalysts.

 The geothermal or hot springs are home to a diverse microbial population. Identification of the thermophilic microbial world of these niches can direct us to the exploration of novel economically valuable biocatalysts, antibiotics, and a variety of other biomolecules. Due to the tectonic features within its borders, Türkiye has many thermal springs in different regions. In this study, a thermal site, the Armutlu geothermal spring in the eastern Marmara region, was investigated to identify the bacterial taxonomic composition and diversity through a metagenomic sequence-based approach which is the first study to the best of our knowledge. During the period of sampling, the water temperature and pH at the surface were 74.9 C and 7.1, respectively. According to 16S rRNA sequencing results, Bacteria 98.36 are the dominant taxa compared to Archaea 1.64. Based on the diversity findings, the bacterial community is primarily dominated by Proteobacteria 42.49, which, together with Chloroflexi, Acidobacteria, Actinobacteria, Firmicutes, Nitrospirae, and Bacteroidetes, constitute 87 of the overall bacterial population. Furthermore, the microbial community was also investigated for the activity of hydrolase enzymes such as protease, amylase, and amylopullulanase through a culture-dependent approach for possible future applications. As a conclusion, the microbiota of the Armutlu geothermal spring was open to investigate the novel species and hydrolytic biocatalysts.

 The geothermal or hot springs are home to a diverse microbial population. Identification of the thermophilic microbial world of these niches can direct us to the exploration of novel economically valuable biocatalysts, antibiotics, and a variety of other biomolecules. Due to the tectonic features within its borders, Türkiye has many thermal springs in different regions. In this study, a thermal site, the Armutlu geothermal spring in the eastern Marmara region, was investigated to identify the bacterial taxonomic composition and diversity through a metagenomic sequence-based approach which is the first study to the best of our knowledge. During the period of sampling, the water temperature and pH at the surface were 74.9 C and 7.1, respectively. According to 16S rRNA sequencing results, Bacteria 98.36 are the dominant taxa compared to Archaea 1.64. Based on the diversity findings, the bacterial community is primarily dominated by Proteobacteria 42.49, which, together with Chloroflexi, Acidobacteria, Actinobacteria, Firmicutes, Nitrospirae, and Bacteroidetes, constitute 87 of the overall bacterial population. Furthermore, the microbial community was also investigated for the activity of hydrolase enzymes such as protease, amylase, and amylopullulanase through a culture-dependent approach for possible future applications. As a conclusion, the microbiota of the Armutlu geothermal spring was open to investigate the novel species and hydrolytic biocatalysts.

Makale detayları

Exploring the Bacterial Diversity of the Armutlu Geothermal Spring (Türkiye) through a Metagenomic Sequenced Based Approach

Yazarlar:

Anıl Cebeci | Merve Öztuğ | Hande Mumcu | Nevin Gül Karagüler

Yayıncı:

Yozgat Bozok University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Bozok Journal of Science Vol.1 Issue 2, pp.57-64

Belge Tipi:

Makale

Yayın Tarihi:

11/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001304429

pISSN:

2980-3314

Bacterial community

Armutlu geothermal spring

Thermophile

16S rRNA sequencing

Hydrolases

Sayı

Makale detayları

Exploring the Bacterial Diversity of the Armutlu Geothermal Spring (Türkiye) through a Metagenomic Sequenced Based Approach

Yazarlar: Anıl Cebeci | Merve Öztuğ | Hande Mumcu | Nevin Gül Karagüler

Yayıncı: Yozgat Bozok University

Ana Başlıklar: Yapısal Biyoloji

Dergi: Bozok Journal of Science Vol.1 Issue 2, pp.57-64

Belge Tipi: Makale

Yayın Tarihi: 11/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001304429

pISSN: 2980-3314

Bacterial community

Armutlu geothermal spring

Thermophile

16S rRNA sequencing

Hydrolases

Sayı

Yazarlar:

Anıl Cebeci | Merve Öztuğ | Hande Mumcu | Nevin Gül Karagüler

Yayıncı:

Yozgat Bozok University

Ana Başlıklar:

Yapısal Biyoloji

Dergi:

Bozok Journal of Science Vol.1 Issue 2, pp.57-64

Belge Tipi:

Makale

Yayın Tarihi:

11/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001304429

pISSN:

2980-3314