Marker-assisted breeding of a durum wheat cultivar for &#947;-gliadin and LMW-glutenin proteins affecting pasta quality

Ahmet YILDIRIM; Özlem ATEŞ SÖNMEZOĞLU; Abdulvahit SAYASLAN

Makale detayları

Marker-assisted breeding of a durum wheat cultivar for γ-gliadin and LMW-glutenin proteins affecting pasta quality

Yazarlar:

Ahmet YILDIRIM | Özlem ATEŞ SÖNMEZOĞLU | Abdulvahit SAYASLAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 5, pp.527-533

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000807557

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

The pasta quality of durum wheat is one of the most important properties for the industry and consumers. Therefore, breeding for improved grain quality without yield penalties, using modern breeding methods, has been a primary objective in durum wheat breeding programs in recent years. In this study, 2 important gene regions that encode pasta-quality associated proteins (/gamma-gliadin 45 and LMW-2 glutenin) were transferred to a registered Turkish durum wheat variety, Sarıçanak-98, from a high-quality Canadian durum wheat cultivar, Kyle, through a marker-assisted backcross breeding method. Each of the F1 and backcross (BC) plants were backcrossed 4 times to the recurrent parent, and the backcrossed plants carrying the targeted gene regions in all generations were selected by marker-assisted selection (MAS). DNA markers in combination with A-PAGE were used for tracking the introgression of the targeted gene regions. Transfer of Gli-B1 locus encoding /gamma-gliadin 45 and Glu-B3 locus encoding LMW-2 glutenin to the wheat variety Sarıçanak-98 led to a considerable increase in protein content and gluten quality.

 

The pasta quality of durum wheat is one of the most important properties for the industry and consumers. Therefore, breeding for improved grain quality without yield penalties, using modern breeding methods, has been a primary objective in durum wheat breeding programs in recent years. In this study, 2 important gene regions that encode pasta-quality associated proteins (/gamma-gliadin 45 and LMW-2 glutenin) were transferred to a registered Turkish durum wheat variety, Sarıçanak-98, from a high-quality Canadian durum wheat cultivar, Kyle, through a marker-assisted backcross breeding method. Each of the F1 and backcross (BC) plants were backcrossed 4 times to the recurrent parent, and the backcrossed plants carrying the targeted gene regions in all generations were selected by marker-assisted selection (MAS). DNA markers in combination with A-PAGE were used for tracking the introgression of the targeted gene regions. Transfer of Gli-B1 locus encoding /gamma-gliadin 45 and Glu-B3 locus encoding LMW-2 glutenin to the wheat variety Sarıçanak-98 led to a considerable increase in protein content and gluten quality.

Makale detayları

Marker-assisted breeding of a durum wheat cultivar for γ-gliadin and LMW-glutenin proteins affecting pasta quality

Yazarlar:

Ahmet YILDIRIM | Özlem ATEŞ SÖNMEZOĞLU | Abdulvahit SAYASLAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 5, pp.527-533

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000807557

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Key words: Durum wheat

Triticum durum

/gamma-gliadin 45

LMW-2 glutenin

backcross breeding

MAS

SSR

Sayı

Makale detayları

Yazarlar: Ahmet YILDIRIM | Özlem ATEŞ SÖNMEZOĞLU | Abdulvahit SAYASLAN

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 5, pp.527-533

Belge Tipi: Makale

Yayın Tarihi: 4/2013

Belge Dili: Türkçe

Document ID (Org No.): 2000807557

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Sayı

Yazarlar:

Ahmet YILDIRIM | Özlem ATEŞ SÖNMEZOĞLU | Abdulvahit SAYASLAN

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 2013 Vol.37 Issue 5, pp.527-533

Belge Tipi:

Makale

Yayın Tarihi:

4/2013

Belge Dili:

Türkçe

Document ID (Org No.):

2000807557

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X