Nanoscale detection of ovarian cancer biomarker

N.A.

Makale detayları

Nanoscale detection of ovarian cancer biomarker

Yazarlar:

N.A.

Yayıncı:

Yildiz Technical University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 6, pp.1884-1891

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001333133

pISSN:

1304-7191

Anahtar kelimeler:

Özet:

 It is estimated that over 2,000 women globally are impacted by ovarian cancer OC. Unfortunately, every year, over half of the cases end in death. This underscores the critical importance of better early detection and diagnosis of OC. Research into improving a sensitive, dependable, and rapid cancer serum antigen CA-125 detector is essential for medical diagnosis. This antigen may signal the earliest stages of ovarian cancer. The typical crystalline size of the Cadmium selenide CdSe quantum dots QDs that we synthesized and coated is adjusted in this study to be 8  0.2 nm. Multiple characterization methods, including X-ray diffraction, Z-scan, and transmission electron microscopy, were utilized to verify their properties. We used these QDs as optical tags for a sandwich assay, which was used to test a high-binding surface where the capture antibody was immobilized. Popular optical tags QDs, semiconductor nanoparticles with size-dependent fluorescence spectra, brightness, and photostability, were used and can be detected by a microplate spectrophotometer. A linear curve was observed within a range of 51000 Uml CA-125, which is suitable for quantitative work, and the detection limit was 4.5 Uml. The interference with the detection of CA-125 was tested in human serum, and the results showed no significant difference in the fluorescence spectra. Third-order nonlinear optical parameter analysis of conjugated CdSe QDs under an excitation beam power level of approximately 120 W and a wavelength of 532 nm was determined in detail. The third-order optical susceptibility χ3 was 2.746  10-7 esu with a sound absorption coefficient of 4.68556  10-5 cmW and nonlinear refractive index -5.6132  10-9 cm2W. Finally, the resulting CdSe QDs have been effectively employed in the labeling and, hence, detection of ovarian cancer antigen CA-125.

 It is estimated that over 2,000 women globally are impacted by ovarian cancer OC. Unfortunately, every year, over half of the cases end in death. This underscores the critical importance of better early detection and diagnosis of OC. Research into improving a sensitive, dependable, and rapid cancer serum antigen CA-125 detector is essential for medical diagnosis. This antigen may signal the earliest stages of ovarian cancer. The typical crystalline size of the Cadmium selenide CdSe quantum dots QDs that we synthesized and coated is adjusted in this study to be 8  0.2 nm. Multiple characterization methods, including X-ray diffraction, Z-scan, and transmission electron microscopy, were utilized to verify their properties. We used these QDs as optical tags for a sandwich assay, which was used to test a high-binding surface where the capture antibody was immobilized. Popular optical tags QDs, semiconductor nanoparticles with size-dependent fluorescence spectra, brightness, and photostability, were used and can be detected by a microplate spectrophotometer. A linear curve was observed within a range of 51000 Uml CA-125, which is suitable for quantitative work, and the detection limit was 4.5 Uml. The interference with the detection of CA-125 was tested in human serum, and the results showed no significant difference in the fluorescence spectra. Third-order nonlinear optical parameter analysis of conjugated CdSe QDs under an excitation beam power level of approximately 120 W and a wavelength of 532 nm was determined in detail. The third-order optical susceptibility χ3 was 2.746  10-7 esu with a sound absorption coefficient of 4.68556  10-5 cmW and nonlinear refractive index -5.6132  10-9 cm2W. Finally, the resulting CdSe QDs have been effectively employed in the labeling and, hence, detection of ovarian cancer antigen CA-125.

 It is estimated that over 2,000 women globally are impacted by ovarian cancer OC. Unfortunately, every year, over half of the cases end in death. This underscores the critical importance of better early detection and diagnosis of OC. Research into improving a sensitive, dependable, and rapid cancer serum antigen CA-125 detector is essential for medical diagnosis. This antigen may signal the earliest stages of ovarian cancer. The typical crystalline size of the Cadmium selenide CdSe quantum dots QDs that we synthesized and coated is adjusted in this study to be 8  0.2 nm. Multiple characterization methods, including X-ray diffraction, Z-scan, and transmission electron microscopy, were utilized to verify their properties. We used these QDs as optical tags for a sandwich assay, which was used to test a high-binding surface where the capture antibody was immobilized. Popular optical tags QDs, semiconductor nanoparticles with size-dependent fluorescence spectra, brightness, and photostability, were used and can be detected by a microplate spectrophotometer. A linear curve was observed within a range of 51000 Uml CA-125, which is suitable for quantitative work, and the detection limit was 4.5 Uml. The interference with the detection of CA-125 was tested in human serum, and the results showed no significant difference in the fluorescence spectra. Third-order nonlinear optical parameter analysis of conjugated CdSe QDs under an excitation beam power level of approximately 120 W and a wavelength of 532 nm was determined in detail. The third-order optical susceptibility χ3 was 2.746  10-7 esu with a sound absorption coefficient of 4.68556  10-5 cmW and nonlinear refractive index -5.6132  10-9 cm2W. Finally, the resulting CdSe QDs have been effectively employed in the labeling and, hence, detection of ovarian cancer antigen CA-125.

 It is estimated that over 2,000 women globally are impacted by ovarian cancer OC. Unfortunately, every year, over half of the cases end in death. This underscores the critical importance of better early detection and diagnosis of OC. Research into improving a sensitive, dependable, and rapid cancer serum antigen CA-125 detector is essential for medical diagnosis. This antigen may signal the earliest stages of ovarian cancer. The typical crystalline size of the Cadmium selenide CdSe quantum dots QDs that we synthesized and coated is adjusted in this study to be 8  0.2 nm. Multiple characterization methods, including X-ray diffraction, Z-scan, and transmission electron microscopy, were utilized to verify their properties. We used these QDs as optical tags for a sandwich assay, which was used to test a high-binding surface where the capture antibody was immobilized. Popular optical tags QDs, semiconductor nanoparticles with size-dependent fluorescence spectra, brightness, and photostability, were used and can be detected by a microplate spectrophotometer. A linear curve was observed within a range of 51000 Uml CA-125, which is suitable for quantitative work, and the detection limit was 4.5 Uml. The interference with the detection of CA-125 was tested in human serum, and the results showed no significant difference in the fluorescence spectra. Third-order nonlinear optical parameter analysis of conjugated CdSe QDs under an excitation beam power level of approximately 120 W and a wavelength of 532 nm was determined in detail. The third-order optical susceptibility χ3 was 2.746  10-7 esu with a sound absorption coefficient of 4.68556  10-5 cmW and nonlinear refractive index -5.6132  10-9 cm2W. Finally, the resulting CdSe QDs have been effectively employed in the labeling and, hence, detection of ovarian cancer antigen CA-125.

Makale detayları

Nanoscale detection of ovarian cancer biomarker

Yazarlar:

N.A.

Yayıncı:

Yildiz Technical University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 6, pp.1884-1891

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001333133

pISSN:

1304-7191

Cancer Serum Antigen (CA-125)

Ovarian Cancer

Quantum dots (QDs)

Sayı

Makale detayları

Nanoscale detection of ovarian cancer biomarker

Yazarlar: N.A.

Yayıncı: Yildiz Technical University

Ana Başlıklar: Clinical Sciences (Other)

Dergi: Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 6, pp.1884-1891

Belge Tipi: Makale

Yayın Tarihi: 9/2024

Belge Dili: İngilizce

Document ID (Org No.): 2001333133

pISSN: 1304-7191

Cancer Serum Antigen (CA-125)

Ovarian Cancer

Quantum dots (QDs)

Sayı

Yazarlar:

N.A.

Yayıncı:

Yildiz Technical University

Ana Başlıklar:

Clinical Sciences (Other)

Dergi:

Sigma Journal of Engineering and Natural Sciences Vol.42 Issue 6, pp.1884-1891

Belge Tipi:

Makale

Yayın Tarihi:

9/2024

Belge Dili:

İngilizce

Document ID (Org No.):

2001333133

pISSN:

1304-7191