Detection of Crown Gal Agent ( Agrobacterium tumefaciens) in  Grapevine Propagating Material

Kemal BENLİOĞLU

Makale detayları

Detection of Crown Gal Agent ( Agrobacterium tumefaciens) in Grapevine Propagating Material

Yazarlar:

Kemal BENLİOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 1998 Vol.22 Issue 2, pp.167-174

Belge Tipi:

Makale

Yayın Tarihi:

1/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000810665

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Anahtar kelimeler:

Özet:

 

Polyclonal antiserum produced against Agrobacterium tumefaciens (biovar 3) reacted in immunofluorescent test with  biovar 3 strains of A.tumefaciens,causal agent of crown gal, from Central Anatolia. The antiserum did not react with A.tumefaciens  biovar 1 (except for 1/200 dilution), biovar 2, other plant pathogens and symbionts. Vacuum extraction procedure were applied to  recover A.tumefaciens from xylem vessels of one year old dormant grape cuttings. IFAS and selective media were used to detect  bacteria. A.tumefaciens (biovar 3) was recovered from six cuttings of 8 infected vines and seven of 150 apparently healthy vines in  Central Anatolia. The sensitivity thershold of the vacuum extraction method and the effectiveness of the IFAS and selective media  were experimentally evaluated. Roots of 1 year old grafted vines of 27 cultivars were also examined for the presence of  A.tumefaciens. Six of these cultivars were contaminated with tumorigenic biovar 3 and seven of these with nontumorigenic biovar  3 or 1. Of 28 strains of A.tumefaciens isolated, 25 were identified as biovar 3, and 3 as biovar 1 according to their physiological  characteristics.

 

Polyclonal antiserum produced against Agrobacterium tumefaciens (biovar 3) reacted in immunofluorescent test with  biovar 3 strains of A.tumefaciens,causal agent of crown gal, from Central Anatolia. The antiserum did not react with A.tumefaciens  biovar 1 (except for 1/200 dilution), biovar 2, other plant pathogens and symbionts. Vacuum extraction procedure were applied to  recover A.tumefaciens from xylem vessels of one year old dormant grape cuttings. IFAS and selective media were used to detect  bacteria. A.tumefaciens (biovar 3) was recovered from six cuttings of 8 infected vines and seven of 150 apparently healthy vines in  Central Anatolia. The sensitivity thershold of the vacuum extraction method and the effectiveness of the IFAS and selective media  were experimentally evaluated. Roots of 1 year old grafted vines of 27 cultivars were also examined for the presence of  A.tumefaciens. Six of these cultivars were contaminated with tumorigenic biovar 3 and seven of these with nontumorigenic biovar  3 or 1. Of 28 strains of A.tumefaciens isolated, 25 were identified as biovar 3, and 3 as biovar 1 according to their physiological  characteristics.

Makale detayları

Detection of Crown Gal Agent ( Agrobacterium tumefaciens) in Grapevine Propagating Material

Yazarlar:

Kemal BENLİOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 1998 Vol.22 Issue 2, pp.167-174

Belge Tipi:

Makale

Yayın Tarihi:

1/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000810665

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X

Turk. J. Agric. For.

22

(1998)

167-174. Full text: pdf Other articles published in the same issue: Turk. J. Agric. For.

vol.22

iss.2.

Sayı

Makale detayları

Detection of Crown Gal Agent ( Agrobacterium tumefaciens) in Grapevine Propagating Material

Yazarlar: Kemal BENLİOĞLU

Yayıncı: TUBITAK

Dergi: Turkish Journal of Agriculture and Forestry 1998 Vol.22 Issue 2, pp.167-174

Belge Tipi: Makale

Yayın Tarihi: 1/1998

Belge Dili: Türkçe

Document ID (Org No.): 2000810665

Sıklık: İki Aylık

eISSN: 1303-6173

pISSN: 1300-011X

Turk. J. Agric. For.

22

(1998)

167-174. Full text: pdf Other articles published in the same issue: Turk. J. Agric. For.

vol.22

iss.2.

Sayı

Yazarlar:

Kemal BENLİOĞLU

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Agriculture and Forestry 1998 Vol.22 Issue 2, pp.167-174

Belge Tipi:

Makale

Yayın Tarihi:

1/1998

Belge Dili:

Türkçe

Document ID (Org No.):

2000810665

Sıklık:

İki Aylık

eISSN:

1303-6173

pISSN:

1300-011X