Immunohistochemical Distribution of Transforming Growth Factor Alpha,  Laminin, Fibronectin and Desmin in Rat Ovary

Gökhan AKKOYUNLU; İsmail ÜSTÜNEL; Ramazan DEMİR

Makale detayları

Immunohistochemical Distribution of Transforming Growth Factor Alpha, Laminin, Fibronectin and Desmin in Rat Ovary

Yazarlar:

Gökhan AKKOYUNLU | İsmail ÜSTÜNEL | Ramazan DEMİR

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.467-482

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

İngilizce

Document ID (Org No.):

2000813987

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

Since the pure synthesis of transforming growth facto alpha (TGFa) many attempts have  been made in order to determine its distribution in various tissues. As a result of the complexitiy of  the cyclic periods throughout experimental models, it is not easy to determine the distribution of TGFa  in the system. Especially the pre-and through-gestational pattern of TGFa in rat ovary has not been  reported fully. Furthermore, the shortage of literature about intracellular desmin and laminin,  fibronectin of different intermediate filaments whether they have any interaction with TGFa through  this period, makes the subject worth discussing.  Immunocytochemical techniques were utilized in order to elucidate the presence and cellular  distribution in rat ovarian tissues of different reproductive stages using monoclonal/polyclonal  antibodies against these proteins.  Our results indicate that the rat ovarial tissue was immunostained for both TGFa and intermediate  filaments. Although TGFa was immunoreactive for all stages from primordial to Graafian follicles, this  reaction was not present in stromal elements. Desmin was localized sparsely through some stromal  cells, blood vessel walls and around developing follicles of day nineteen pregnant rat ovary. Laminin  was localized at the basal laminae of thecal cells and blood vessel endothelia. Fibronectin  immunoreactivity was observed commonly around the luteal cells.  The results of this study show evidence for the immunolocalization of TGFa and intermediate filaments  in rat ovarian tissue in various phases of the menstrual cycle which indicate their presence in this tissue  and suggest a role in rat ovarian function.

 

Since the pure synthesis of transforming growth facto alpha (TGFa) many attempts have  been made in order to determine its distribution in various tissues. As a result of the complexitiy of  the cyclic periods throughout experimental models, it is not easy to determine the distribution of TGFa  in the system. Especially the pre-and through-gestational pattern of TGFa in rat ovary has not been  reported fully. Furthermore, the shortage of literature about intracellular desmin and laminin,  fibronectin of different intermediate filaments whether they have any interaction with TGFa through  this period, makes the subject worth discussing.  Immunocytochemical techniques were utilized in order to elucidate the presence and cellular  distribution in rat ovarian tissues of different reproductive stages using monoclonal/polyclonal  antibodies against these proteins.  Our results indicate that the rat ovarial tissue was immunostained for both TGFa and intermediate  filaments. Although TGFa was immunoreactive for all stages from primordial to Graafian follicles, this  reaction was not present in stromal elements. Desmin was localized sparsely through some stromal  cells, blood vessel walls and around developing follicles of day nineteen pregnant rat ovary. Laminin  was localized at the basal laminae of thecal cells and blood vessel endothelia. Fibronectin  immunoreactivity was observed commonly around the luteal cells.  The results of this study show evidence for the immunolocalization of TGFa and intermediate filaments  in rat ovarian tissue in various phases of the menstrual cycle which indicate their presence in this tissue  and suggest a role in rat ovarian function.

Makale detayları

Immunohistochemical Distribution of Transforming Growth Factor Alpha, Laminin, Fibronectin and Desmin in Rat Ovary

Yazarlar:

Gökhan AKKOYUNLU | İsmail ÜSTÜNEL | Ramazan DEMİR

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.467-482

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

İngilizce

Document ID (Org No.):

2000813987

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Rat

ovary

transforming growth factor alpha (TGFa)

laminin

desmin

fibronectin

immunohistochemistry.

Sayı

Makale detayları

Yazarlar: Gökhan AKKOYUNLU | İsmail ÜSTÜNEL | Ramazan DEMİR

Yayıncı: TUBITAK

Ana Başlıklar: Yaşam bilimleri

Dergi: Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.467-482

Belge Tipi: Makale

Yayın Tarihi: 2/2000

Belge Dili: İngilizce

Document ID (Org No.): 2000813987

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Sayı

Yazarlar:

Gökhan AKKOYUNLU | İsmail ÜSTÜNEL | Ramazan DEMİR

Yayıncı:

TUBITAK

Ana Başlıklar:

Yaşam bilimleri

Dergi:

Turkish Journal of Biology 2000 Vol.24 Issue 3, pp.467-482

Belge Tipi:

Makale

Yayın Tarihi:

2/2000

Belge Dili:

İngilizce

Document ID (Org No.):

2000813987

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152