Activated expression of EsHD1 enhances drought tolerance in tobacco plants via mitigation of reactive oxygen species-mediated membrane damage

Cheng ZHOU; Zhongyou MA; Lin ZHU; Jiansheng GUO; Xianghuan CUI; Jian ZHU; Jianfei WANG

Makale detayları

Activated expression of EsHD1 enhances drought tolerance in tobacco plants via mitigation of reactive oxygen species-mediated membrane damage

Yazarlar:

Cheng ZHOU | Zhongyou MA | Lin ZHU | Jiansheng GUO | Xianghuan CUI | Jian ZHU | Jianfei WANG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 6, pp.941-951

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815641

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Anahtar kelimeler:

Özet:

 

It is well documented that the homeodomain-leucine zipper (HD-Zip) transcription factors play diverse roles during plant growth and development. The Class I HD-Zip genes are shown to be involved in the regulation of abiotic stress responses and tolerance. Herein, a novel Class I HD-Zip gene, EsHD1, was isolated from Eutrema salsugineum, and an investigation was performed of its physiological functions in response to drought stress. The analyses of gene expression profiles revealed that the EsHD1 transcripts quickly accumulated upon exposure to various abiotic stress conditions or abscisic acid treatments. Furthermore, the overexpression of EsHD1 in tobacco plants conferred improved tolerance to drought stress. The EsHD1-overexpressing lines had lower levels of reactive oxygen species (ROS), ion leakage, and malondialdehyde, but they manifested higher activities of antioxidant enzymes and the transcription of pathogenic-related genes as compared to wild-type plants under drought stress conditions. Therefore, our findings demonstrated that EsHD1 positively regulated plant drought tolerance via alleviation of ROS-mediated membrane damage.

 

It is well documented that the homeodomain-leucine zipper (HD-Zip) transcription factors play diverse roles during plant growth and development. The Class I HD-Zip genes are shown to be involved in the regulation of abiotic stress responses and tolerance. Herein, a novel Class I HD-Zip gene, EsHD1, was isolated from Eutrema salsugineum, and an investigation was performed of its physiological functions in response to drought stress. The analyses of gene expression profiles revealed that the EsHD1 transcripts quickly accumulated upon exposure to various abiotic stress conditions or abscisic acid treatments. Furthermore, the overexpression of EsHD1 in tobacco plants conferred improved tolerance to drought stress. The EsHD1-overexpressing lines had lower levels of reactive oxygen species (ROS), ion leakage, and malondialdehyde, but they manifested higher activities of antioxidant enzymes and the transcription of pathogenic-related genes as compared to wild-type plants under drought stress conditions. Therefore, our findings demonstrated that EsHD1 positively regulated plant drought tolerance via alleviation of ROS-mediated membrane damage.

Makale detayları

Activated expression of EsHD1 enhances drought tolerance in tobacco plants via mitigation of reactive oxygen species-mediated membrane damage

Yazarlar:

Cheng ZHOU | Zhongyou MA | Lin ZHU | Jiansheng GUO | Xianghuan CUI | Jian ZHU | Jianfei WANG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 6, pp.941-951

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815641

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X

Eutrema salsugineum

HD-Zip

drought stress

membrane stability

transgenic tobacco

Sayı

Makale detayları

Activated expression of EsHD1 enhances drought tolerance in tobacco plants via mitigation of reactive oxygen species-mediated membrane damage

Yazarlar: Cheng ZHOU | Zhongyou MA | Lin ZHU | Jiansheng GUO | Xianghuan CUI | Jian ZHU | Jianfei WANG

Yayıncı: TUBITAK

Dergi: Turkish Journal of Botany 2015 Vol.39 Issue 6, pp.941-951

Belge Tipi: Makale

Yayın Tarihi: 12/2015

Belge Dili: Türkçe

Document ID (Org No.): 2000815641

Sıklık: İki Aylık

eISSN: 1303-6106

pISSN: 1300-008X

Eutrema salsugineum

HD-Zip

drought stress

membrane stability

transgenic tobacco

Sayı

Yazarlar:

Cheng ZHOU | Zhongyou MA | Lin ZHU | Jiansheng GUO | Xianghuan CUI | Jian ZHU | Jianfei WANG

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Botany 2015 Vol.39 Issue 6, pp.941-951

Belge Tipi:

Makale

Yayın Tarihi:

12/2015

Belge Dili:

Türkçe

Document ID (Org No.):

2000815641

Sıklık:

İki Aylık

eISSN:

1303-6106

pISSN:

1300-008X