Ashwagandha root extract attenuates inflammation in Oleic acid induced-ALI/ARDS rat model via inhibition of ACE and MAPK signaling pathways

Kubra Koc

Makale detayları

Ashwagandha root extract attenuates inflammation in Oleic acid induced-ALI/ARDS rat model via inhibition of ACE and MAPK signaling pathways

Yazarlar:

Kubra Koc

Yayıncı:

Mevlüt AKÇURA

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 1, pp.196-202

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283232

pISSN:

2148-3647

Anahtar kelimeler:

Özet:

 Ashwagandha Withania somniferous is one of the most important plants of folk medicine and is widely used to treat various diseases. Acute lung injury ALI and acute respiratory distress syndrome ARDS are defined as a respiratory failure that abruptly develops due to hypoxemia with alveolar injury secondary to intense inflammation. The present study was focused on evaluating the activity of Ashwagandha against Oleic Acid-Induced ALIARDS in a rat model. For this purpose, the animals were divided into the following three groups: Control, Oleic acid 50 μl kg1, i.v. injection, Ashwagandha 500 mgkg, orally  Oleic acid. Ashwagandha was given daily for two weeks before a single dose of the Oleic acid. 24 hours after the last application, all the group animals were sacrificed by sevoflurane, and their lung was evaluated. The levels of Mitogen-activated protein kinases MAPK, and the activities of myeloperoxidase MPO, glutathione GSH, superoxide dismutase SOD, total oxidant status TOS, and angiotensin-converting enzyme ACE were determined in lung tissues by ELISA. Compared with the model group, there was a significantly improving in the levels of MAPK, MPO, and TOS in the Ashwagandha administration group. Moreover, Ashwagandha markedly increased the activities of GSH and SOD, and decreased the activity of ACE. Therefore, Ashwagandha may be used as a potential natural resource for mitigating acute lung injury caused by Oleic acid.

 Ashwagandha Withania somniferous is one of the most important plants of folk medicine and is widely used to treat various diseases. Acute lung injury ALI and acute respiratory distress syndrome ARDS are defined as a respiratory failure that abruptly develops due to hypoxemia with alveolar injury secondary to intense inflammation. The present study was focused on evaluating the activity of Ashwagandha against Oleic Acid-Induced ALIARDS in a rat model. For this purpose, the animals were divided into the following three groups: Control, Oleic acid 50 μl kg1, i.v. injection, Ashwagandha 500 mgkg, orally  Oleic acid. Ashwagandha was given daily for two weeks before a single dose of the Oleic acid. 24 hours after the last application, all the group animals were sacrificed by sevoflurane, and their lung was evaluated. The levels of Mitogen-activated protein kinases MAPK, and the activities of myeloperoxidase MPO, glutathione GSH, superoxide dismutase SOD, total oxidant status TOS, and angiotensin-converting enzyme ACE were determined in lung tissues by ELISA. Compared with the model group, there was a significantly improving in the levels of MAPK, MPO, and TOS in the Ashwagandha administration group. Moreover, Ashwagandha markedly increased the activities of GSH and SOD, and decreased the activity of ACE. Therefore, Ashwagandha may be used as a potential natural resource for mitigating acute lung injury caused by Oleic acid.

 Ashwagandha Withania somniferous is one of the most important plants of folk medicine and is widely used to treat various diseases. Acute lung injury ALI and acute respiratory distress syndrome ARDS are defined as a respiratory failure that abruptly develops due to hypoxemia with alveolar injury secondary to intense inflammation. The present study was focused on evaluating the activity of Ashwagandha against Oleic Acid-Induced ALIARDS in a rat model. For this purpose, the animals were divided into the following three groups: Control, Oleic acid 50 μl kg1, i.v. injection, Ashwagandha 500 mgkg, orally  Oleic acid. Ashwagandha was given daily for two weeks before a single dose of the Oleic acid. 24 hours after the last application, all the group animals were sacrificed by sevoflurane, and their lung was evaluated. The levels of Mitogen-activated protein kinases MAPK, and the activities of myeloperoxidase MPO, glutathione GSH, superoxide dismutase SOD, total oxidant status TOS, and angiotensin-converting enzyme ACE were determined in lung tissues by ELISA. Compared with the model group, there was a significantly improving in the levels of MAPK, MPO, and TOS in the Ashwagandha administration group. Moreover, Ashwagandha markedly increased the activities of GSH and SOD, and decreased the activity of ACE. Therefore, Ashwagandha may be used as a potential natural resource for mitigating acute lung injury caused by Oleic acid.

 Ashwagandha Withania somniferous, halk hekimliğinin en önemli bitkilerinden biridir ve çeşitli hastalıkları tedavi etmek için yaygın olarak kullanılmaktadır. Akut akciğer hasarı AAH ve akut solunum sıkıntısı sendromu ASSS, yoğun inflamasyona sekonder alveoler hasar ile birlikte hipoksemiye bağlı olarak ani gelişen solunum yetmezliği olarak tanımlanır. Mevcut çalışma, Ashwagandhanın Oleik Asit kaynaklı AAHASSSna karşı aktivitesini sıçan modelinde değerlendirmeye odaklanmıştır. Bu amaçla hayvanlar, aşağıdaki üç gruba ayrılmıştır: Kontrol, Oleik asit 50 µl kg-1, i.v. enjeksiyon, Ashwagandha 500 mgkg, oral  Oleik asit. Ashwagandha, tek Oleik asit dozundan önce iki hafta boyunca günlük olarak verilmiştir. Son uygulamadan 24 saat sonra gruptaki tüm hayvanlar sevofluran ile sakrifiye edilerek akciğerleri değerlendirilmiştir. Mitojenle-etkinleşen protein kinazlar MAPKın seviyeleri ve miyeloperoksidaz MPO, glutatyon GSH, süperoksit dismutaz SOD, total oksidan durum TOS ve anjiyotensin dönüştürücü enzim ACE aktiviteleri akciğer dokularıda ELISA ile belirlenmiştir. Model grubu ile karşılaştırıldığında, Ashwagandha uygulanan grupta MAPK, MPO ve TOS seviyelerinde önemli bir düzelme olmuştur. Ayrıca Ashwagandha, GSH ve SOD aktivitelerini önemli ölçüde arttırırken, ACE aktivitesini azaltmıştır. Bu nedenle Ashwagandha, Oleik asitin neden olduğu akut akciğer hasarını hafifletmek için potansiyel bir doğal kaynak olarak kullanılabilir.

Makale detayları

Ashwagandha root extract attenuates inflammation in Oleic acid induced-ALI/ARDS rat model via inhibition of ACE and MAPK signaling pathways

Yazarlar:

Kubra Koc

Yayıncı:

Mevlüt AKÇURA

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 1, pp.196-202

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283232

pISSN:

2148-3647

Ashwagandha

TOS

MAPK

lung injury

ACE activity

Ashwagandha

akciğer hasarı

TOS

MAPK

ACE aktivitesi

Sayı

Makale detayları

Yazarlar: Kubra Koc

Yayıncı: Mevlüt AKÇURA

Dergi: Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 1, pp.196-202

Belge Tipi: Makale

Yayın Tarihi: 1/2023

Belge Dili: İngilizce

Document ID (Org No.): 2001283232

pISSN: 2148-3647

Sayı

Yazarlar:

Kubra Koc

Yayıncı:

Mevlüt AKÇURA

Dergi:

Turkish Journal of Agricultural and Natural Sciences Vol.10 Issue 1, pp.196-202

Belge Tipi:

Makale

Yayın Tarihi:

1/2023

Belge Dili:

İngilizce

Document ID (Org No.):

2001283232

pISSN:

2148-3647