Phylogenetic analysis of peste des petits ruminants virus (PPRV) isolated in Iran based on partial sequence data from the fusion (F) protein gene

Majid ESMAELIZAD; Saber JELOKHANI-NIARAKI; Rohani KARGAR-MOAKHAR

Makale detayları

Phylogenetic analysis of peste des petits ruminants virus (PPRV) isolated in Iran based on partial sequence data from the fusion (F) protein gene

Yazarlar:

Majid ESMAELIZAD | Saber JELOKHANI-NIARAKI | Rohani KARGAR-MOAKHAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2011 Vol.35 Issue 1, pp.45-50

Belge Tipi:

Makale

Yayın Tarihi:

12/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000812956

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Anahtar kelimeler:

Özet:

 

In order to gain some insight into the origin of the peste des petits ruminants virus (PPRV) isolated in Iran, a selected region of the PPRV genome in a clinical sample collected from a sheep was amplified using RT-PCR, and the resulting amplicon was sequenced for phylogenetic analysis. The partial nucleotide and predicted amino acid sequence of the Iranian isolate was aligned with the corresponding sequences of 16 previously published F genes. Sequence analysis of the strains showed that the Iranian isolate had the highest degree of homology with the majority of the strains, with the exception of Nigerian isolates and ICV89. In general, a higher degree of amino acid sequence conservation was observed among the various strains of field PPRV. Phylogenetic comparison of the Iranian isolate, along with some published exotic sequences, indicated that the virus has been circulating for years in Iran's neighboring countries, including Turkey and Pakistan. Overall analysis of the amino acid and nucleotide substitutions showed that ICV89 from Ivory Coast was more prone to sequence alterations than the others were. The phylogenetic tree created for F protein nucleotide data was divided into 4 separate lineages. All strains were conserved within the cleavage site of the amino acid sequence, except for Mdn96. Analysis of the sequence data showed that PPRV circulation has been homologous in Asian countries. Determination of the nucleotide sequence of the partial F protein of the Iranian isolate would help clarify the origin of the disease.

 

In order to gain some insight into the origin of the peste des petits ruminants virus (PPRV) isolated in Iran, a selected region of the PPRV genome in a clinical sample collected from a sheep was amplified using RT-PCR, and the resulting amplicon was sequenced for phylogenetic analysis. The partial nucleotide and predicted amino acid sequence of the Iranian isolate was aligned with the corresponding sequences of 16 previously published F genes. Sequence analysis of the strains showed that the Iranian isolate had the highest degree of homology with the majority of the strains, with the exception of Nigerian isolates and ICV89. In general, a higher degree of amino acid sequence conservation was observed among the various strains of field PPRV. Phylogenetic comparison of the Iranian isolate, along with some published exotic sequences, indicated that the virus has been circulating for years in Iran's neighboring countries, including Turkey and Pakistan. Overall analysis of the amino acid and nucleotide substitutions showed that ICV89 from Ivory Coast was more prone to sequence alterations than the others were. The phylogenetic tree created for F protein nucleotide data was divided into 4 separate lineages. All strains were conserved within the cleavage site of the amino acid sequence, except for Mdn96. Analysis of the sequence data showed that PPRV circulation has been homologous in Asian countries. Determination of the nucleotide sequence of the partial F protein of the Iranian isolate would help clarify the origin of the disease.

Makale detayları

Phylogenetic analysis of peste des petits ruminants virus (PPRV) isolated in Iran based on partial sequence data from the fusion (F) protein gene

Yazarlar:

Majid ESMAELIZAD | Saber JELOKHANI-NIARAKI | Rohani KARGAR-MOAKHAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2011 Vol.35 Issue 1, pp.45-50

Belge Tipi:

Makale

Yayın Tarihi:

12/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000812956

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152

Key words: PPRV

sequence

phylogenetic analysis

isolate

Sayı

Makale detayları

Phylogenetic analysis of peste des petits ruminants virus (PPRV) isolated in Iran based on partial sequence data from the fusion (F) protein gene

Yazarlar: Majid ESMAELIZAD | Saber JELOKHANI-NIARAKI | Rohani KARGAR-MOAKHAR

Yayıncı: TUBITAK

Dergi: Turkish Journal of Biology 2011 Vol.35 Issue 1, pp.45-50

Belge Tipi: Makale

Yayın Tarihi: 12/2010

Belge Dili: Türkçe

Document ID (Org No.): 2000812956

Sıklık: İki Aylık

eISSN: 1303-6092

pISSN: 1300-0152

Key words: PPRV

sequence

phylogenetic analysis

isolate

Sayı

Yazarlar:

Majid ESMAELIZAD | Saber JELOKHANI-NIARAKI | Rohani KARGAR-MOAKHAR

Yayıncı:

TUBITAK

Dergi:

Turkish Journal of Biology 2011 Vol.35 Issue 1, pp.45-50

Belge Tipi:

Makale

Yayın Tarihi:

12/2010

Belge Dili:

Türkçe

Document ID (Org No.):

2000812956

Sıklık:

İki Aylık

eISSN:

1303-6092

pISSN:

1300-0152